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Research Article
Another giant species of the microhylid frog genus Cophixalus Boettger, 1892 from the mountains of Papua New Guinea and first records of procoracoids in the genus
expand article infoRainer Günther, Chris Dahl§, Stephen J. Richards|
‡ Museum für Naturkunde, Berlin, Germany
§ New Guinea Binatang Research Center, Madang, Papua New Guinea
| South Australian Museum, Adelaide, Australia
Open Access

Abstract

A new arboreal species of the microhylid genus Cophixalus Boettger, 1892 is described from montane rainforest on Papua New Guinea’s central cordillera. With a male SUL exceeding 44.0 mm, the new species is among the largest members of the genus; the only other Papuan species known to reach this size is C. riparius Zweifel, 1962. The new species differs from C. riparius in a small number of mensural characters and by its distinct advertisement call, a single explosive ‘bark’ uttered singly or in rapid series. In contrast, calls of C. riparius recorded near the type locality are a series of drawn out, rasping croaks. Calls of the two species are analysed and compared. The two species also appear to have different ecologies, with the new species found only high in trees, while C. riparius is often encountered in vegetation on or near the forest floor. Examination of osteological features revealed the presence of cartilaginous procoracoids in both species, representing the first records of procoracoids in the speciose genus Cophixalus. Lack of procoracoids is traditionally considered an important diagnostic character for defining Cophixalus but both species also lack clavicles, a character considered diagnostic for Cophixalus and a key feature distinguishing the genus from the closely related Oreophryne Boettger, 1895. Because preliminary published genetic data indicate that they are nested within Cophixalus, we retain both species in that genus until a comprehensive molecular phylogeny of Cophixalus and related genera, particularly Oreophryne, is completed.

Key Words

Amphibia, bioacoustics, central cordillera, montane rainforest, morphology, New Guinea, taxonomy

Introduction

Microhylid frogs in the asterophryine genus Cophixalus are confined to the Australopapuan region, where they reach their greatest diversity on mainland New Guinea (Menzies 2006; Kraus and Allison 2009a, b; Kraus 2012; Hill et al. 2022). The genus exhibits substantial morphological diversity, reflecting the broad range of terrestrial (Günther 2006; Kraus and Allison 2009a; Günther and Richards 2011), scansorial (Kraus and Allison 2009a; Richards and Günther 2019) and arboreal (Zweifel 1962; Menzies 2006) habitats that the species occupy. However, the relationship between this observed morphological divergence and the species’ phylogenetic relationships remains unclear (Kraus 2012). Despite this morphological diversity, the majority of Cophixalus species from New Guinea are tiny to small frogs; the males of many species do not exceed 20 mm in body length and few reach 30 mm (Günther 2006; Kraus and Allison 2006; Menzies 2006; Kraus and Allison 2009a, b; Kraus 2012). Exceptions include C. caverniphilus Kraus & Allison, 2009 with males reaching 30.2 mm, and the Sudest Island population of C. verrucosus (Boulenger, 1898) with males reaching 31.0 mm (Kraus and Allison 2009b; Kraus 2012). Records of C. cryptotympanum Zweifel, 1956 from the mountains of western Papua New Guinea reaching up to 40 mm (presumably females but sex was not noted; Menzies 2006) are based on misidentifications (Richards and Günther 2019), and the taxonomic status of those populations requires further assessment.

The only Cophixalus from the island of New Guinea that is known to have a male body size exceeding 40 mm is C. riparius. It was described from a series of 224 specimens collected in 1959 by Hobart van Deusen at an altitude of 2,775 m a.s.l. on the east slopes of Mt Wilhelm in Chimbu Province, Papua New Guinea (Zweifel 1962). As currently understood C. riparius is moderately widespread at altitudes between 1,900–2,800 m a.s.l. in the mountains of central Papua New Guinea where its known distribution extends in a narrow band from Southern Highlands and Western Highlands Provinces in the west to the Schrader Mountains in Madang Province in the north and to the vicinity of Wau in Morobe Province in the southeast (Zweifel 1962; Kraus 2010; IUCN SSC Amphibian Specialist Group 2020). Recent surveys to document the altitudinal distribution of amphibians at several sites on Mt Wilhelm provided the opportunity to obtain data on the advertisement calls of C. riparius from the vicinity of the type locality. Calls of C. riparius from Mt Wilhelm differ markedly from those produced by a morphologically similar but geographically isolated population documented recently on Gigira Ridge in Hela Province approximately 260 km to the west of the type locality (Richards et al. 2021). Here we describe this western population as a species distinct from C. riparius and report the presence of cartilaginous procoracoids in both species. These are the first records of cartilaginous procoracoids in the genus Cophixalus.

Materials and methods

Male frogs of the new species were located at night by their advertisement calls but only one adult male and one juvenile specimen could be collected. These vouchers were anaesthetised in an aqueous chlorobutanol solution and subsequently fixed in 5% formalin. Both specimens were transferred to 70% ethanol within two days of fixation. The following measurements were taken with a digital calliper (> 10 mm) or with a binocular dissecting microscope fitted with an ocular micrometer (< 10 mm) to the nearest 0.1 mm from preserved specimens using protocols for microhylid frogs adopted previously (e.g. Günther et al. 2014): SUL – snout-urostyle length from tip of snout to posterior tip of urostyle (SUL is sufficiently similar to SVL that, where relevant, we compare our SUL measurements with SVLs presented for members of the genus in some papers); TL – tibia length: external distance between knee and tibio-tarsal articulation; TaL – length of tarsus: external distance between tibio-tarsal and tarsal-metatarsal joints held at right angles; FTL – length of foot, from tip of 4th toe to proximal edge of sole; T4D – transverse diameter of disc of 4th toe; T1D – transversal diameter of disc of first toe; HDL – length of hand, from tip of 3rd finger to proximal edge of palm; F3D – transverse diameter of disc of 3rd finger; F1D– transversal diameter of disc of first finger; HL – head length, from tip of snout to posterior margin of tympanum; HW – head width, taken in the region of the tympana; SL – snout length, from an imaginary line connecting the centres of the eyes to tip of the snout; END – distance from anterior corner of orbital opening to centre of naris; IND – internarial distance between centres of nares; ED – eye diameter, from anterior to posterior corner of orbital opening; EST – distance from anterior corner of orbital opening to tip of snout; TyD – horizontal diameter of tympanum. Measurements are presented as mean ± standard deviation and range.

Advertisement calls were recorded under natural conditions with a Roland R-05 digital recorder and Sennheiser ME-66 shotgun microphone and analysed with Avisoft-SAS Lab Pro software. Air temperatures were taken ~2 m above the forest floor, directly below calling males. Terminology and acoustic analysis procedures follow Köhler et al. (2017).

The colour of animals in life was described from digital photographs, and of preserved specimens from direct observations. Most colours were determined according to a colour matching system that is created and administrated by the German RAL GmbH (RAL non-profit LLC) available at https://en.wikipedia.org/wiki/RAL_colour_standard. When it was impossible to find an exact match between observed colour and a certain RAL colour number the most similar RAL number was chosen.

Due to the small sample size of the new species, osteological characters were examined by partial dissection and staining with alcian blue. Osteological features of comparative specimens in the genera Cophixalus and Oreophryne were determined using this method, or specimens were cleared and stained according to Dingerkus and Uhler (1977).

The holotype and a juvenile paratype of the new species are stored in the collection of the South Australian Museum Adelaide (SAMA). Acronyms for additional institutions mentioned herein are: AMNH (American Museum of Natural History, New York) and ZMB (Museum für Naturkunde, Berlin).

Compared material

The following specimens of Cophixalus riparius, the only very large Cophixalus species previously known from New Guinea, were examined: six paratypes from the type locality, Pengagl Creek on the east slope of Mt Wilhelm, Chimbu Province, Papua New Guinea (AMNH A.112985, A.112992, A.112995, A.113004–5, and A.113007); four additional specimens collected recently by C. Dahl from the north-eastern slopes of Mt Wilhelm within 13 km of the type locality (SAMA R71660–63); one specimen collected by S. Richards and C. Dahl from Mt Michael near Goroka in Eastern Highlands Province (SAMA R71701); and 53 specimens collected by F. Parker from the Porol Range, 30 km SSE of Mt Wilhelm (ZMB 42575–628). Additional voucher specimens, including types, of the genus Cophixalus that were studied for comparative purposes are listed in the papers by Richards et al. (1992), Günther (2003, 2006, 2010), Richards and Oliver (2007, 2010), Günther and Richards (2011) and Günther et al. (2014) and additional comparative information was taken from original descriptions and recompiled treatises (Méhely 1901; Zweifel 1956a, b, 1962, 1979; Tyler 1963; Zweifel and Parker 1989; Kraus and Allison 2006, 2009a, b; Menzies 2006; Kraus 2012; Richards and Günther 2019).

Results

The new species is assigned to the genus Cophixalus based on having the jaw eleutherognathine, clavicles absent, third toe longer than fifth, discs on fingers broader than on toes, and snout not elongated and lacking pad of connective tissue. The new species and C. riparius have cartilaginous procoracoids (see below), the lack of which has previously been considered a diagnostic character for Cophixalus, but genetically they are nested within Cophixalus (Richards et al. 2021) so we assign them to that genus pending further studies.

Cophixalus gigiraensis sp. nov.

Mount Gigira Giant Nursery Frog

Oreophryne ? sp. 5 ‘loud grunter’ (Richards & Armstrong, 2017).

Cophixalus sp. 5 ‘loud grunter’ (Richards & Armstrong, 2018).

Cophixalus sp. 4 ‘loud grunter’ (Richards et al., 2021).

Type material

Holotype : SAMA R71740 (SJR 15394), adult male from Gigira Ridge, Hela Province, Papua New Guinea (5.9725°S, 142.7532°E; 2160 m a.s.l.) collected on 22 May 2017 by S. Richards and C. Dahl.

Paratype : SAMA R71700 (FN SJR15396), juvenile, same details as holotype except collected on 23 May 2017.

Referred specimens

SAMA R71748 (FN SJR[JCUNQ]5097), Summit ridge of Mt Sisa, Hela Province, Papua New Guinea (6.1343°S, 142.7536°E; 2450 m a.s.l.) collected on 29 October 1999 by S. Richards; SAMA R71749 (FN SJR8645), Summit of Mount Elimbari, Chimbu Province, Papua New Guinea (6.1899°S, 145.1486°E; 2566 m a.s.l.) collected on 01 December 2004 by S. Richards and C. Dahl.

Diagnosis

With a snout-urostyle length of 44.4 mm in an adult male the new species is among the largest species of the genus; in New Guinea only Cophixalus riparius reaches a similar size. It is distinguished from C. riparius and all other congeners by the following unique combination of characters: body robust, head short (HL/SUL 0.31); legs moderately short (TL/SUL 0.42), third toe longer than fifth; fingers and toes with greatly expanded triangular terminal discs, all with circum-marginal grooves; discs of fingers much larger than those of toes (T4D/F3D 0.76); dorsal surfaces including rear of tarsus with scattered low tubercles, ventral surfaces smooth; most of dorsal surfaces brown-olive (RAL 8008) with irregular beige (most similar to RAL 1001) flecking; ventral surfaces whitish overlain with moderately dense reddish-brown pigmentation; advertisement call a loud explosive ‘bark’ produced singly or in groups of up to eight, each containing 13–19 pulses lasting 60–80 ms, dominant frequency 1.5 kHz.

Description of the holotype

(Fig. 1a–d). An adult male with vocal slits, calling when collected. For measurements see Table 1. Head slightly broader than long (HL/HW 0.89), canthus rostralis rounded; loreal region steep, slightly concave; snout protruding in profile, slightly pointed in dorsal view; nostrils directed dorsolaterally, closer to tip of snout than to eyes; horizontal eye diameter much greater than eye-naris distance (ED/END 1.48); tympanum relatively small (TyD/ED 0.37), not well-demarcated; supratympanic skin fold narrow but well defined in life and preservative; internarial distance greater than distance between eye and naris (END/IND 0.83); tongue large, pear-shaped, posterior margin rounded and free; two prepharyngeal ridges, anterior ridge with 10 tiny lobes, posterior one with 14 denticles; vocal slits moderately long, located on both sides of tongue. Legs relatively short (TL/SUL 0.42); webbing between fingers and toes absent; discs of fingers triangular, wider than triangular discs of toes (T4D/F3D 0.76); all finger and toe discs with circum-marginal grooves; relative length of fingers 3>4>2>1 (Fig. 1c). Third toe slightly longer than fifth; relative length of toes 4>3>5>2>1 (Fig. 1d); most subarticular, metatarsal and metacarpal tubercles indicated by light colour but structurally only scarcely developed. In life dorsal surfaces and posterior of tarsus with scattered low tubercles; all ventral surfaces smooth.

Figure 1. 

Holotype of Cophixalus gigiraensis sp. nov. in life a. Dorsolateral view; b. Ventral view; c. Palmar view of right hand; d. Plantar view of right foot.

Table 1.

Body measurements and body ratios of the male holotype (SAMA R71740) and a juvenile paratype (SAMA R71700) of Cophixalus gigiraensis sp. nov. All measurements in mm; for explanation of abbreviations see Material and methods section.

Reg.No SAMA R 71740 SAMA R 71700
SUL 44.4 15.4
TL 18.5 7.6
TaL 12.5 4.5
T4L 19.6 3.9
T4D 2.5 0.8
T1D 2.2 0.6
F3L 15.0 3.1
F3D 3.3 0.9
F1D 2.5 0.6
HL 13.7 5.8
HW 15.4 5.7
END 3.3 1.6
IND 4.0 1.8
SL 6.5 3.4
ED 4.9 2.5
EST 5.2 2.2
TyD 1.8 0.9
TL/SUL 0.42 0.49
TaL/SUL 0.28 0.29
T4L/SUL 0.44 0.25
T4D/SUL 0.056 0.052
F3L/SUL 0.34 0.20
F3D/SUL 0.074 0.058
T4D/F3D 0.76 0.89
T1D/F1D 0.88 1.00
HL/SUL 0.31 0.38
HW/SUL 0.35 0.37
HL/HW 0.89 1.02
END/SUL 0.074 0.104
IND/SUL 0.090 0.117
END/IND 0.83 0.89
ED/SUL 0.110 0.162
EST/SUL 0.117 0.143
TyD/SUL 0.041 0.058
TyD/ED 0.37 0.36
SL/SUL 0.146 0.221

Dorsal surfaces in life predominantly clay-brown (RAL 8003) (Fig. 1a), paler on flanks than mid-dorsum; mid-dorsum, upper flanks and incomplete lumbar spots with several irregular beige flecks; upper arms including elbows, and tarsi including tibio-tarsal joints, also with beige flecking; ventral surfaces whitish with more (on extremities) or less (on abdomen, chest and throat) dense reddish-brown pigmentation (Fig. 1b); rear of thighs monochromatic pale brown (RAL 8025). Iris whitish with sparse network of dark brown reticulations.

In preservative dorsal and lateral surfaces darker brown, beige flecks less obvious prior to staining with alcian blue. After staining, dorsal surfaces uniformly slate gray (RAL 7015) with pale brown (RAL 8025) areas detectable through the gray; dorsal surfaces of hands beige brown (RAL 8024); throat pale brown with soft blue tinge; chest and abdomen with pronounced blue tinge; ventral surfaces of extremities predominantly pale brown.

Description of the paratype

(Fig. 2a–c). A juvenile (SUL 15.4 mm) of undetermined sex. For measurements see Table 1. General body and head shape, and presence of low tubercles on dorsum and tarsi similar to holotype but colour pattern in life strikingly different. Head and nape in dorsolalateral and dorsal views with large light ivory (RAL 1015) blotch (Fig. 2a, b). This blotch continues anteriorly to nostrils where it is interrupted at tip of snout by triangular brown-grey (RAL 7013) spot; and posteriorly to urostyle. Light ivory area framed dorsolaterally by two irregularly shaped gray-brown (RAL 8019) stripes extending from behind eyes to well-defined lumbar spots. Additional light ivory areas on dorsal surfaces of upper arms, around tibio-tarsal articulations, on dorsal surfaces of tarsi and on upper flanks. Snout partially beige-red (RAL 3012) dorsally and laterally. Dorsal surfaces of thighs and shanks, lower flanks, anterior forearm and subocular region olive grey (RAL 7002). Throat, chest, abdomen and lower surface of thighs rather uniform olive grey with few irregular whitish spots (Fig. 2c). Iris pebble grey (RAL 7032) with dark brown reticulations (Fig. 2a). Several morphological ratios of the juvenile paratype deviate substantially from those of the adult paratype (Table 1). Further material is required to determine whether these differences represent ontogenetic change.

Figure 2. 

a–c. Juvenile paratype of Cophixalus gigiraensis sp. nov. in life.

Vocalisation

The advertisement call of Cophixalus gigiraensis is a short, harsh barking note uttered singly or in series containing 2–8 notes (Fig. 3a–c). Eight calls (two single calls and three sets of couplets) of the holotype were of sufficient quality for analysis. Intervals between these note=call series were 28–37 s but many other frogs were heard calling at longer intervals of up to several minutes (S. Richards, personal observations). The number of pulses and length of calls is difficult to assess exactly, due to an echo possibly associated with their calling position inside small holes in tree trunks, but these eight notes=calls contain 8–17 pulses (mean 14.6±3.2), some incompletely divided, lasting approximately 68–106 ms (mean 78.5±12.4 ms). Intervals between calls produced in couplets are 812–910 ms (n = 3); dominant frequency is at 1.5 kHz and there is a weak fundamental frequency band at 0.6 kHz (Fig. 3b, c). An unvouchered specimen uttered three call series in a row consisting of 5, 8 and 7 calls. These calls were much shorter than those produced by the holotype (50–77 ms, mean 64.7±6.4 ms, n=20) and intercall intervals were also shorter (240–349 ms, mean 266.9±29.0 ms, n=17) than in the calls from the holotype.

Figure 3. 

Oscillogram (a), spectrogram (b) and relative amplitude (c) of an advertisement call series from the holotype of Cophixalus gigiraensis sp. nov. consisting of two calls. Basic noise was deleted up to 0.3 kHz. Sampling rate conversion from 24 kHz to 12 kHz; spectrogram parameters: FFT length 256, Frame size 75%, Window FlatTop, Bandwidth 313 Hz, resolution 63 Hz, Overlap 87.5%.

Distribution

Cophixalus gigiraensis is known with certainty only from Gigira (Hides) Ridge at the northern edge of the Kikori River basin in Hela Province, Papua New Guinea (Fig. 4). It may also occur on nearby Mount Sisa (Richards and Armstrong 2018; this location falls within the yellow square indicating the type locality in Fig. 4), and on Mount Elimbari about 270 km east of the type locality (Richards et al. 2021) but resolution of the status of those populations will require collection of additional material. If the Mount Elimbari population is confirmed to belong to C. gigiraensis then this will represent a large range extension and demonstrate that this species occurs in close proximity to its close relative C. riparius. Cophixalus gigiraensis was not found at lower altitudes elsewhere in the Kikori River basin, despite intensive surveys on the Agogo Range at altitudes between 1,000 and 1,700 m a.s.l. over many years.

Figure 4. 

Distribution of Cophixalus gigiraensis sp. nov. (yellow square) and C. riparius (blue circles). The arrow indicates the type locality of C. riparius.

Habitat and habits

Cophixalus gigiraensis is an arboreal species that calls at night from ~15–30 m high in Nothofagus trees in mid-montane rainforest on karst terrain (Fig. 5). It is not possible to safely climb these large trees so, although the species appears to be moderately abundant because numerous specimens were heard calling in the vicinity of the type locality in 2005 and again between 2015 and 2019, only one adult male and one juvenile have been captured. The adult male was calling from a height of about six metres at the entrance to a small hole in the vertical trunk of a large Nothofagus tree, while the juvenile was on low foliage in the forest understorey at night. The new species occurs in sympatry with six other microhylid frog species: Choerophryne brevicrus (Günther & Richards, 2012), two undescribed Choerophryne species, Hylophorbus richardsi Günther, 2001, Oreophryne anamiatoi Kraus & Allison, 2009 and O. notata Zweifel, 2003, the limnodynastid Platyplectrum aganoposis (Zweifel, 1972) and the pelodryadids Litoria iris (Tyler, 1962) and L. vivissimmia Oliver, Richards & Donnellan, 2019.

Figure 5. 

Emergent Nothofagus trees in montane forest on Gigira Ridge. Male Cophixalus gigiraensis called from the upper canopy of these large emergent trees making collection difficult.

Etymology

The specific epithet gigiraensis refers to the type locality of this species, Gigira Ridge, otherwise known as Hides Ridge. Gigira is the local Huli Community’s name for this mountain.

Comparison with other species

Only one other species of Cophixalus on New Guinea, C. riparius (Fig. 6), reaches the size of C. gigiraensis. Cophixalus riparius was described by Zweifel in 1962 on the basis of a large series (224 specimens including the holotype) from the east slope of Mt Wilhelm in Western Highlands Province.

Figure 6. 

A male Cophixalus riparius (SAMA R71661) from near the type locality of this species on Mt Wilhelm, Papua New Guinea. The dorsal colour pattern of this species is highly variable.

The holotype of C. gigiraensis differs biometrically from five randomly selected adult males and five adult females from the Porol riparius-series in having a longer head (HL/SUL 0.31 vs. 0.26–0.30), a higher HL/HW ratio (0.89 vs. 0.72–0.88), and a broader disc on the first toe (T1D/SUL 0.050 vs. 0.040–0.049; T1D/F1D 0.88 vs. 0.63–0.77). Males from the Porol Range are somewhat smaller than females (14 adult males measured 37.8–41.1 mm SUL and 10 adult females 41.2–47.1 mm SUL). Sexual size dimorphism is common among many anuran species but has rarely been recorded for species in the genus Cophixalus. This also suggests that C. gigiraensis may be slightly larger than C. riparius, but additional material of the new species is required to confirm this.

The advertisement calls of C. riparius and the new species are different (compare Figs 3, 7). A series of four calls produced by a C. riparius (SAMA R71660) near the type locality on Mt Wilhelm are long, guttural croaks lasting 751–1031 ms (mean 927 ms) and contain 34–35 pulses. Calls in the series are separated by intervals of 4.5–5.4 s. A conspicuous feature of these calls is that pulse rate is much faster at the beginning of the call than at the end (Fig. 7a, b). Dominant frequency is at 1.55 kHz (Fig. 7c). A second call series produced by a C. riparius (SAMA R71701) on Mt Michael, about 70 km SSW of the type locality, is of poorer quality but in all pertinent respects agrees with calls produced by the near-topotypic animal. It also contains four long, rasping calls, and pulse rate declines markedly during each call. In contrast, the short, barking calls of C. gigiraensis last just 50–106 ms, contain 8–17 pulses, and pulse rate does not change during the call; call intervals within series last 0.24–0.90 s.

Figure 7. 

Oscillogram (a), spectrogram (b) and relative amplitude (c) of an advertisement call of Cophixalus riparius from Mt Wilhelm consisting of 35 pulses. Basic noise was deleted up to 0.3 kHz. Sampling rate conversion from 24 kHz to 16 kHz; spectrogram parameters: FFT length 256, Frame size 75%, Window FlatTop, Bandwidth 313 Hz, resolution 63 Hz, Overlap 87.5%.

There also appear to be some ecological differences between the two species. The large series of C. riparius collected at the type locality by Hobart Van Deusen (> 200 specimens “amid grass, low shrubs, or boulders”) and by Fred Parker in the Porol Range suggests that not only was the species abundant at those locations but that they were commonly encountered on or near the forest floor. In contrast, C. gigiraensis appears to be restricted to the mid and upper canopy of Nothofagus trees (Fig. 5). The only animal encountered near the ground during 1.5 months of survey effort in the vicinity of the type locality, spread over several years (2015–2019), was the juvenile paratype. It was perched on a leaf approximately 2 m above the ground in the forest understorey. The adult holotype was the lowest-calling individual heard during numerous surveys, being only ~6 m above the forest floor. It was collected by binding several long saplings together to dislodge it from its arboreal calling perch.

Procoracoids in the genus Cophixalus

According to Parker (1934), Zweifel and Parker (1989), Burton and Zweifel (1995), Kraus and Allison (2000, 2009a), Kraus (2012) and Richards and Günther (2019) one of the key features defining the genus Cophixalus is the lack of procoracoids. During investigation of the ventral elements of the pectoral girdle to determine generic allocation of the new species, we discovered that the holotype of C. gigiraensis and several randomly chosen specimens of C. riparius from the Porol Range all have cartilaginous procoracoids. Their structure is similar to that of Oreophryne species wherein the procoracoids do not reach the scapulae. Lateral processes of procoracoids in a preserved and partly dissected specimen of C. riparius stained with alcian blue (ZMB 42612, Fig. 8a) and in two cleared and double stained specimens (ZMB 42593 and ZMB 42625) of this species are longer (reaching roughly to middle of the coracoid bone) and narrower than in the holotype of C. gigiraensis, and the shape of the proximal part is flat in C. riparius from the Porol Range but high (triangular) in C. gigiraensis (compare Fig. 8a, b). Based on the existing preparations it is not possible to determine whether the procoracoids are connected directly with the sternum or whether an omosternum is a component of the structure of proximal parts of the procoracoids.

Figure 8. 

(a) Ventral view of procoracoids of Cophixalus riparius (ZMB 42612) from the Porol Range and (b) ventral view of procoracoids of Cophixalus gigiraensis sp. nov. (SAMA R71740). (A) lateral process of the right procoracoid, (B) omosternum (?), (C) coracoid, (D) sternum, arrows in Fig. 8a mark anterior and posterior edges of coracoids.

It is possible that Zweifel (1962) did not detect these small and fragile elements in C. riparius because most specimens examined internally were done so via dissection, and only the absence of a clavicle was confirmed in two cleared and stained specimens (Zweifel 1962). It is also possible that the Porol Range material does not represent C. riparius, but the large series from that location examined by us is indistinguishable from topotypic C. riparius. We do not speculate further on this discrepancy other than to note that further investigations of these structures in topotypic C. riparius are required. Studies of additional material are also required to determine whether the observed differences in the shape of the procoracoids between three C. riparius from the Porol Range and one C. gigiraensis are species specific.

Discussion

The presence or absence of both procoracoids and clavicles has traditionally been a key morphological feature used to identify and classify Australopapuan microhylid frogs (Parker 1934). However, there appear to have been multiple independent losses of procoracoids and clavicles among lineages of Australopapuan frogs (e.g., Burton 1990), and recent studies have indicated that Oreophryne (defined by traditional morphological characters) comprises two unrelated clades (Hill et al. 2022) so that the loss of procoracoids and clavicles has occurred at least twice in this group. Indeed, increased use of molecular phylogenetic techniques in combination with the rapidly increasing number of Australopapuan microhylid species available for study (Oliver et al. 2022) have demonstrated that many of the morphological traits traditionally used to classify this fauna at the genus level may be phylogenetically uninformative due to convergence (Hill et al. 2022). Cophixalus riparius was not included in recent molecular phylogenetic studies of New Guinean microhylid frogs (Köhler and Günther 2008; Peloso et al. 2016; Rivera et al. 2017; Hill et al. 2022) but a preliminary study of the relationships among more than 100 microhylid species using genome-scale DNA sequencing placed both C. riparius and C. gigiraensis (as Cophixalus cf. riparius) within Cophixalus, not Oreophryne (Richards et al. 2021). Further studies are required to determine whether the presence of procoracoids and clavicles is more widespread among the rapidly increasing known diversity of Australopapuan Cophixalus. Interestingly, the two species closest to riparius and gigiraensis in the molecular tree presented by Richards et al. (2021), Cophixalus nubicola Zweifel, 1962 and C. kaindiensis Zweifel, 1979 are both reported to lack these elements (Zweifel 1962, 1979).

Cophixalus gigiraensis and C. riparius are the largest Cophixalus in New Guinea, with male SVL of both species exceeding 40 mm. They are morphologically similar but acoustically distinct, and phylogenetic analyses of SNP data support their distinctiveness (Richards et al. 2021; C. gigiraensis presented as Cophixalus cf. riparius). The two species also appear to be ecologically divergent, with C. gigiraensis normally calling from more than 15 m, and commonly up to 30 m, above the forest floor in large Nothofagus trees on karst terrain (Fig. 5; Richards & Dahl, unpublished observations). In contrast, although Kraus and Allison (2000) reported C. riparius from epiphytes up to 20 m above the ground, large numbers of C. riparius have also been encountered on or near the ground (grass, low shrubs and boulders; Zweifel 1962), in habitats quite unlike those encountered at the type locality of C. gigiraensis. Chomiki (2020) also reported C. riparius inhabiting the epiphytic ant-plant Hydnophytum myrtifolium in the highlands of Papua New Guinea, but erroneously implied that this species was breeding in the accumulated rainwater within the ant plants. Like other Australopapuan microhylid frogs C. riparius and C. gigiraensis almost certainly have direct development, in which embryos develop directly into small juveniles, bypassing the tadpole stage (Anstis et al. 2011). It is not known whether C. gigiraensis also occupies the ant plants that occur at the type locality.

The description of Cophixalus gigiraensis from the limestone terrain of Gigira Ridge adds to the growing number of frog species known predominantly or entirely from karst habitats along the southern fringe of New Guinea’s Central Cordillera (Richards and Oliver 2010; Richards and Günther 2019; Richards et al. in press). However, additional information on the distribution of C. gigiraensis is required before it can be determined whether this species is confined to karst habitats or is more broadly distributed at suitable altitudes across this mountainous region.

Acknowledgements

SJR expresses his gratitude to The PNG National Research Institute who assisted with his Research Visa, and the PNG Department of Environment and Conservation (now Conservation and Environment Protection Authority) for approving the export of specimens. Carolyn Kovach, Domenic Capone, Sally South, and Mark Hutchinson provided access to material, registration numbers, and numerous other courtesies at the South Australian Museum and Linda Ford and Darrel Frost kindly provided access to specimens in their care at the American Museum of Natural History. Frank Tillack and Oskar Werb kindly supported RG at the Museum für Naturkunde, Berlin. Elke Günther (Berlin) and Lisa Capon (Speewah) kindly assisted with the production and editing of several of the figures. Field work on Gigira Ridge was supported by ExxonMobil PNG Limited (EMPNG), and surveys on Mt Wilhelm and Mt Michael were supported by the New Guinea Binatang Research Center and Conservation International respectively. SJR and CD are most grateful for their support. Paul Oliver and Allen Allison provided useful comments that greatly improved the manuscript.

References

  • Anstis MF, Parker T, Hawkes I, Morris I, Richards SJ (2011) Direct development in some Australopapuan microhylid frogs of the genera Austrochaperina, Cophixalus and Oreophryne (Anura: Microhylidae) from northern Australia and Papua New Guinea. Zootaxa 3052(1): 1–50. https://doi.org/10.11646/zootaxa.3052.1.1
  • Burton TC (1990) The New Guinea genus Copiula Mehelÿ (Anura: Microhylidae): a new diagnostic character and a new species. Transactions of the Royal Society of South Australia 114: 87–93.
  • Burton TC, Zweifel RG (1995) A new genus of genyophrynine microhylid frogs from New Guinea. American Museum Novitates 3129: 1–7.
  • Günther R (2003) First record of the microhylid frog genus Cophixalus from western Papua, Indonesia, with descriptions of two new species (Anura: Microhylidae). Herpetozoa (Wien) 16: 3–21.
  • Günther R (2006) Two new tiny Cophixalus species with reduced thumbs from the west of New Guinea (Anura: Microhylidae). Herpetozoa (Wien) 19: 59–75.
  • Günther R (2010) Another new Cophixalus species (Amphibia: Anura: Microhylidae) from western New Guinea. Bonn Zoological Bulletin 57: 231–240.
  • Günther R, Richards SJ (2011) Five new microhylid frog species from Enga Province, Papua New Guinea, and remarks on Albericus alpestris (Anura, Microhylidae). Vertebrate Zoology 61: 343–372.
  • Günther R, Richards SJ, Dahl C (2014) Nine new species of microhylid frogs from the Muller Range in western Papua New Guinea (Anura, Microhylidae). Vertebrate Zoology 64: 59–94.
  • Hill EC, Fraser CJ, Gao DF, Jarman MJ, Henry ER, Iova B, Allison A, Butler MA (2022) Resolving the deep phylogeny: Implications for early adaptive radiation, cryptic, and present-day ecological diversity of Papuan microhylid frogs. Molecular Phylogenetics and Evolution 177: 107618. https://doi.org/10.1016/j.ympev.2022.107618
  • Köhler F, Günther R (2008) The radiation of microhylid frogs (Amphibia: Anura) on New Guinea: A mitochondrial phylogeny reveals parallel evolution of morphological and life history traits and disproves the current morphology-based classification. Molecular Phylogenetics and Evolution 47(1): 353–365. https://doi.org/10.1016/j.ympev.2007.11.032
  • Köhler J, Jansen M, Rodrнguez A, Kok PJR, Toledo LF, Emmrich M, Glaw F, Haddad CFB, Rцdel MO, Vences M (2017) The use of bioacoustics in anuran taxonomy: Theory, terminology, methods and recommendations for best practice. Zootaxa 4251(1): 1–124. https://doi.org/10.11646/zootaxa.4251.1.1
  • Kraus F (2010) More range extensions for Papuan reptiles and amphibians. Herpetological Review 41: 246–248.
  • Kraus F, Allison A (2006) Three new species of Cophixalus (Anura: Microhylidae) from southeastern New Guinea. Herpetologica 62(2): 202–220. https://doi.org/10.1655/05-09.1
  • Méhely Lv (1901) Beiträge zur Kenntnis der Engystomatiden von Neu-Guinea. Természetrajzi Füzetek 24: 169–271.
  • Menzies JI (2006) The frogs of New Guinea and the Solomon Islands. Pensoft, Sofia-Moscow, 345 pp.
  • Oliver PM, Bower D, McDonald PJ, Kraus F, Luedtke J, Neam K, Hobin L, Chauvenet ALM, Allison A, Arida E, Clulow S, Günther R, Nagombi E, Tjaturadi B, Travers SL, Richards SJ (2022) Melanesia holds the world`s most diverse and intact insular amphibian fauna. Communications Biology 5: 1–10. https://doi.org/10.1038/s42003-022-04105-1
  • Parker HW (1934) A Monograph of the Frogs of the Family Microhylidae. British Museum (Natural History), London, U.K.
  • Peloso PLV, Frost DR, Richards SJ, Rodrigues MT, Donnellan SC, Matsui M, Raxworthy CJ, Biju SD, Lemmon EM, Lemmon AR, Wheeler WC (2016) The impact of anchored phylogenomics and taxon sampling on phylogenetic inference in narrow-mouthed frogs (Anura, Microhylidae). Cladistics 32(2): 113–140. https://doi.org/10.1111/cla.12118
  • Richards SJ, Armstrong KN (2017) Chapter 2 – Frogs. In: Richards SJ (Ed.) Biodiversity assessment of the PNG LNG Upstream Project Area, Southern Highlands and Hela Provinces, Papua New Guinea. ExxonMobil PNG Limited. Port Moresby, 53–90.
  • Richards SJ, Armstrong KN (2018) Frogs. In: Richards SJ (Ed.) Identification guide to flora and fauna of Hides Ridge and the Agogo Range (Moro), Papua New Guinea. ExxonMobil PNG Limited. Port Moresby, 49–81.
  • Richards SJ, Günther RG (2019) Three new scansorial species of microhylid frogs (Anura: Cophixalus, Oreophryne) from Papua New Guinea. Salamandra (Frankfurt) 55: 55–72.
  • Richards SJ, Oliver PM (2010) A new scansorial species of Cophixalus (Anura: Microhylidae) from the Kikori River Basin, Papua New Guinea. Journal of Herpetology 44(4): 555–562. https://doi.org/10.1670/09-044.1
  • Richards SJ, Johnston GR, Burton TC (1992) A new species of microhylid frog (genus Cophixalus) from the Star Mountains, central New Guinea. Science in New Guinea 18: 141–145.
  • Richards SJ, Armstrong KN, Nagombi E, Dahl G (2021) Chapter 1 – Frogs. In: Richards SJ (Ed.) Results of the third PMA3 Biodiversity Monitoring Survey of the PNG LNG Upstream Project Area, 8 August–2 September 2019. ExxonMobil PNG Limited. Port Moresby, 19–51.
  • Richards SJ, Donnellan SC, Oliver PM (in press) Five new species of the pelodryadid genus Litoria Tschudi from the southern versant of Papua New Guinea’s Central Cordillera, with observations on the diversification of reproductive strategies in Melanesian treefrogs. Zootaxa.
  • Rivera JA, Kraus F, Allison A, Butler MA (2017) Molecular phylogenetics and dating of the problematic New Guinea microhylid frogs (Amphibia: Anura) reveals elevated speciation rates and need for taxonomic reclassification. Molecular Phylogenetics and Evolution 112: 1–11. https://doi.org/10.1016/j.ympev.2017.04.008
  • Tyler MJ (1963) A taxonomic study of amphibians and reptiles of the central highlands of New Guinea, with notes on their ecology and biology. 1. Anura: Microhylidae. Transactions of the Royal Society of South Australia 86: 11–29.
  • Zweifel RG (1956a) Results of the Archbold Expeditions. No. 72, Microhylid frogs from New Guinea with descriptions of new species. American Museum Novitates 1766: 1–49.
  • Zweifel RG (1956b) Notes on Microhylid Frogs, genus Cophixalus, from New Guinea. American Museum Novitates 1785: 1–8.
  • Zweifel RG (1962) Results of the Archbold Expeditions. No. 83. Frogs of the microhylid genus Cophixalus from the mountains of New Guinea. American Museum Novitates 2087: 1–26.
  • Zweifel RG, Parker F (1989) New species of microhylid frogs from the Owen Stanley Mountains of Papua New Guinea and resurrection of the genus Aphantophryne. American Museum Novitates 2954: 1–20.
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