Research Article |
Corresponding author: Fahrettin Küçük ( fkucuk59@gmail.com ) Academic editor: Nicolas Hubert
© 2021 Fahrettin Küçük, Yılmaz Çiftçi, Salim Serkan Güçlü, Davut Turan.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Küçük F, Çiftçi Y, Güçlü S, Turan D (2021) Chondrostoma smyrnae, a new nase from the Tahtalı reservoir drainage in the Aegean Sea basin (Teleostei, Leuciscidae). Zoosystematics and Evolution 97(1): 235-248. https://doi.org/10.3897/zse.97.63691
|
Chondrostoma smyrnae, a new species, from the Tahtalı reservoir drainage is distinguished by having a slightly arched lower jaw with a well-developed keratinised edge, a deep and cylindric body, a complete lateral line with 47–52+1 total scales, 8–9 scale rows between the lateral line and the dorsal-fin origin, 4 scale rows between the lateral line and the pelvic fin-origin, and 19–23 gill rakers on the first gill arch. Moreover, molecular analyses using full cyt b (1141 bp) and partial coI (652 bp) sequences of the mitochondrial genome from specimens of the new species, C. smyrnae and specimens belonging to other Chondrostoma species from central and western Anatolia demonstrated that the C. smyrnae is easily differentiated by their high pairwise genetic distances of cyt b and coI data set (>2.20 and 1.03%, respectively) and by their position in the phylogenetic trees obtained through Maximum Likelihood (ML) methodology.
Cytochrome b, Cytochrome oxidase I, freshwater fish, taxonomy, Western Anatolia
Nases of the genus Chondrostoma Agassiz, 1832 are medium-to large-sized leuciscid fishes geographically widespread from France to the Volga River and Central Iran (
Currently, 14 species of the genus lives in Turkey, of which eight are endemic to Anatolia (
During a revision project of the genus Chondrostoma in Turkey, we already described three new Chondrostoma species suggested as evolutionary lineages by
The care of experimental animals was consistent with the Republic of Turkey animal welfare laws, guidelines and policies approved by Süleyman Demirel University Local Ethics Committee for Animal Experiments (permit reference number 2011/6/5). Samples were collected by electroscoker. After anaesthesia, samples of caudal fin tissue taken from each specimen for the molecular analysis were fixed and stored in 98% ethanol and fish were fixed in 4% formaldehyde. Measurements were made with a dial caliper and recorded to 1 mm. All measurements were made point-to-point, never by projections. Methods for counts and measurements follow
For osteological preparations, one specimen of a new species (168.8 mm SL) and one specimen of C. turnai (139.4 mm SL) were cleared and stained with alizarin red S, according to the protocol of
SL standard length;
BI Bayesian Inference;
ML Maximum Likelihood;
mt mitochondrial.
Collection codes:
IFC-ESUF Inland Fishes Collection, Eğirdir Fisheries Faculty of Isparta University of Applied Sciences;
FFR Recep Tayyip Erdogan University Zoology Museum of the Faculty of Fisheries, Rize;
FSJF Fischsammlung J. Freyhof, Berlin.
Total DNA was isolated from ethanol-preserved tissue samples using the Invitrogen PureLink Genomic DNA Mini Kit according to the manufacturer’s instructions and stored at −20 °C prior to use. The mitochondrial cytochrome b (cyt b) gene (1141 bp) was amplified using Forward (5’– AAT GAC TTG AAG AAC CAC CGT-3’) and Reverse (5’– CAA CGA TCT CCG GTT TAC AAG AC-3’) (
For the phylogenetic analyses, two data sets were used, an 1140 bp fragment of cyt b and 652 bp fragment of coI sequences. Cyt b and coI sequences were aligned with the previous sequences from GenBank (Table
List of Chondrostoma species analysed, sampling locality, sources and GenBank accession numbers.
Species | River/Drainage | Country | Sources | Accession numbers | |
---|---|---|---|---|---|
Cyt b | CoI | ||||
C. holmwoodii | Gölmarmara/ Akpınar | Turkey |
|
MT387078 | |
Gediz river/ Derbent | Turkey |
|
MT387099 | ||
Bakırçay/ Bergama | Turkey |
|
AF533765 | ||
Gediz river/ Uşak | Turkey |
|
AF533762 | ||
Bakır stream | Turkey |
|
KJ553066.1 | ||
Gediz river /Uşak | Turkey | This study | MW719600 | ||
Gediz river /Uşak | Turkey | This study | MW719598 | ||
Gediz river /Uşak | Turkey | This study | MW719599 | ||
Bakır stream | Turkey |
|
KJ552962.1 | ||
C. smyrnae | Şaşal stream/ Tahtalı | Turkey | This study | MT387055 | MW722822 |
Şaşal stream/ Tahtalı | Turkey | This study | MT387056 | MW722823 | |
Şaşal stream/ Tahtalı | Turkey | This study | MT387057 | MW722824 | |
Şaşal stream/ Tahtalı | Turkey | This study | MT387058 | ||
C. turnai | Çine stream | Turkey |
|
MT387091 | |
Çine stream | Turkey |
|
MT387093 | ||
Çine stream | Turkey |
|
MT387092 | ||
Çine stream | Turkey |
|
MT387095 | ||
Çine stream | Turkey |
|
MT387094 | ||
Büyük Menderes | Turkey |
|
KJ553058.1 | ||
Yayla lake/Buldan | Turkey | This study | MW719601 | ||
Yayla lake/Buldan | Turkey | This study | MW719602 | ||
Yayla lake/Buldan | Turkey | This study | MW719603 | ||
Büyük Menderes | Turkey |
|
KJ553026.1 | ||
Büyük Menderes | Turkey |
|
KJ552989.1 | ||
C. angorense | Akin stream/ Sakarya | Turkey |
|
MT387066 | |
Porsuk stream/ Sakarya | Turkey |
|
MT387114 | ||
Porsuk stream/ Sakarya | Turkey |
|
MT387112 | ||
Kızılırmak river Kırşehir | Turkey | Perea et al. (2010) | HM560078 | ||
Porsuk stream/ Sakarya | Turkey |
|
MT387113 | ||
Sakarya river/ Kızılcahamam | Turkey | This study | MW719604 | ||
Sakarya river/ Kızılcahamam | Turkey | This study | MW719605 | ||
Kızılırmak river/ Zara | Turkey | This study | MW719606 | ||
Kızılırmak river/Zara | Turkey | This study | MW719607 | ||
Delice stream/Yerköy | Turkey | This study | MW719608 | ||
Delice stream /Yerköy | Turkey | This study | MW719609 | ||
Kızılırmak river/ Boyabat | Turkey | This study | MW719610 | ||
Kızılırmak river/ Boyabat | Turkey | This study | MW719611 | ||
C. meandrense | Işıklı spring/ Çivril | Turkey |
|
MT387085 | |
Işıklı spring/ Çivril | Turkey |
|
MT387087 | ||
Işıklı spring/ Çivril | Turkey |
|
MT387088 | ||
Işıklı spring/ Çivril | Turkey |
|
MT387086 | ||
Büyük Menderes | Turkey |
|
KJ553109 | ||
Büyük Menderes /Çivril | Turkey | This study | MW719591 | ||
Büyük Menderes/ Çivril | Turkey | This study | MW719592 | ||
Büyük Menderes/ Dinar | Turkey | This study | MW719593 | ||
Büyük Menderes/ Dinar | Turkey | This study | MW719594 | ||
Büyük Menderes/ Dinar | Turkey | This study | MW719595 | ||
Büyük Menderes/ Akçay | Turkey | This study | MW719596 | ||
Büyük Menderes | Turkey |
|
KJ553083 | ||
C. nasus | Diina River | Serbia | Schönhuth et al. (2014) | MG806833 | |
Soca drainage | Slovenia |
|
KJ553248 | ||
Simav drainage | Turkey |
|
KJ552881 | ||
Danube River | Romania |
|
MF135845 | ||
Susurluk, Kocacay | Turkey |
|
AF533760 | ||
Danube River | Austria |
|
AY026402 | ||
Simav Stream, Yanıkburnu | Turkey |
|
MT387104 | ||
Simav Stream, Yanıkburnu | Turkey |
|
MT387105 | ||
Simav Stream, Yanıkburnu | Turkey |
|
MT387106 | ||
Simav Stream, Bigadiç | Turkey |
|
MT387109 | ||
Simav Stream, Bigadiç | Turkey |
|
MT387110 | ||
C. beysehirense | Beyşehir lake | Turkey |
|
MT387079 | |
Beyşehir lake | Turkey |
|
MT387080 | ||
Beyşehir drainage | Turkey |
|
KJ552898 | ||
Beyşehir lake | Turkey | This study | MW719597 | ||
C. fahirae | Tefenni Burdur | Turkey |
|
MT387128 | |
Pseudochondrostoma willkommii | KF529128 | KJ554279 | |||
Achondrostoma arcasii | KF529113 | KJ552518 |
The nucleotide sequences of both strands resulted in the full length of mitochondrial cyt b (1141 bp) for the samples. Four haplotype sequences belonging to Chondrostoma smyrnae were compared with other Chondrostoma sequences from Western Anatolia available from GenBank (Table
Pairwise distance of cyt b and coI data set within and among Chondrostoma species based on 1.000 bootstrap replications using the Kimura 2-parameter distance method; values in lower left cells = percent difference among taxa for cyt b, values in upper right cells = percent difference among taxa for coI and diagonal = percent difference within taxa for cyt b/coI.
C. holmwoodii | C. smyrnae | C. turnai | C. angorense | C. meandrense | C. nasus | C. beysehirense | |
---|---|---|---|---|---|---|---|
C. holmwoodii | 0.53 /0.12 | 1.19 | 1.47 | 0.77 | 0.95 | 0.93 | 0.85 |
C. smyrnae | 2.95 | 0.13 /0.21 | 1.32 | 1.03 | 1.21 | 1.19 | 1.11 |
C. turnai | 2.91 | 2.20 | 0.33 /0.09 | 1.01 | 1.18 | 1.16 | 1.08 |
C. angorense | 2.47 | 2.61 | 2.48 | 0.35 /0.00 | 0.48 | 0.46 | 0.38 |
C. meandrense | 2.87 | 2.77 | 2.67 | 1.26 | 0.26 /0.04 | 0.64 | 0.56 |
C. nasus | 2.96 | 2.64 | 2.45 | 1.47 | 1.53 | 0.50 /0.00 | 0.54 |
C. beysehirense | 3.29 | 2.86 | 3.00 | 2.30 | 2.28 | 2.18 | 0.26 /0.15 |
C. fahirae | 9.67 | 9.88 | 9.95 | 9.18 | 8.75 | 9.36 | 9.08 |
The dataset for cytochrome c oxidase subunit 1 (coI) included 24 individuals sequenced by this study and eight individuals from GenBank belonging to seven Chondrostoma species (Chondrostoma smyrnae, C. turnai, C. meandrense, C. holmwoodii, C. beysehirense, C. angorense and C. nasus) in western Anatolia (Table
Phylogenetic relationships among the sequences were reconstructed independently for cyt b and coI genes using the ML method. In the phylogenetic reconstructions for cyt b, Chondrostoma haplotypes formed three distinct monophyletic clades, hereafter referred to as clades I, II, and III as in Figure
For coI, the phylogenetic tree demonstrated that Chondrostoma species are divided into four clades (Figure
All phylogenetic analysis showed that these species are separated from one another. Also, C. smyrnae individuals from the Tahtalı reservoir drainage in the Aegean Sea basin are concluded to be distinct members of the Chondrostoma species studied in all phylogenetic trees.
1 | 12–14 gill rakers on first gill arch; no keratinised cutting edge on lower jaw | C. fahirae |
– | 19–28 gill rakers on first gill arch; a keratinised cutting edge on lower jaw | 2 |
2 | Lateral line with 56–68+2–3 scales | 3 |
– | Lateral line with 43–52+1–2 scales | 5 |
3 | Lower jaw markedly arched and keratin not well developed | C. meandrense |
– | Lower jaw slightly arched and keratin is well developed | 4 |
4 | 5–7 scale rows between lateral line and pelvic-fin origin. In life, a longitudinal violet stripe (band) in middle of flank from caudal-fin base to dorsal-fin base | C. holmwoodii |
– | 5 scale rows between lateral line and pelvic-fin origin. Not a longitudinal violet stripe in life (band) | C. angorense |
5 | Body width at dorsal-fin origin 13–15% SL; body width at mid-point of caudal peduncle 4–6% SL | C. turnai |
– | Body width at dorsal-fin origin 16–19% SL; body width at mid-point of caudal peduncle 6–8% SL | C. smyrnae |
IFC-ESUF 03-1566, 191 mm SL; Turkey: İzmir prov.: Tahtalı reservoir about 2 km north of Değirmendere, 38°08'19"N, 27°07'10"E.
IFC-ESUF 03-1567, 22, 152–205 mm SL; IFC-ESUF 03-1568, 22, 181–272 mm SL; same data as holotype. – IFC-ESUF 03-1550, 2, 92.68–109.02 mm SL; Turkey: İzmir prov.: stream Şaşal about 1 km south of Küner, 38°11'57"N, 27°08'09"E. – FFR 2079, 1, 92 mm SL; Turkey: İzmir prov.: stream Balaban at Küner, 38.213950 27.101505.
Chondrostoma smyrnae is distinguished from other species occur to adjacent basin by a cylindrical body (body width at dorsal-fin origin 16.8–19.3% SL, vs. 13.3–15.4 in C. turnai (Fig.
a. Chondrostoma smyrnae, IFC-ESUF 03–1567, just after fixation form, 167 mm SL, Turkey: Tahtalı reservoir; b. IFC-ESUF 03–1566, holotype, 191 mm SL, Turkey: Tahtalı reservoir; c. IFC-ESUF 03–1567, paratype 205 mm SL, Turkey: Tahtalı reservoir; d. Chondrostoma turnai, IFC-ESUF 03–1557, 197 mm SL, Turkey: Çine stream.
The new species is also distinguished from C. meandrense by the body colour silvery in life (silvery, vs. brownish). It is further distinguished from C. holmwoodii by having 8–9 scale rows between the lateral line and dorsal-fin origin (vs. 9–11), four scale rows between the lateral line and pelvic-fin origin (vs. 6–7). Chondrostoma smyrnae is further distinguished from C. fahirae by having of 19–23 gill rakers on the first gill arch (vs. 12–14), well keratinised cutting edge present on the lower jaw (vs. slightly) (Fig.
Ventral view of head: a. C. smyrnae, IFC-ESUF 03–1567, paratype, 189 mm SL, Turkey: Tahtalı reservoir; b. C. turnai, IFC-ESUF 03–1524, 174 mm SL, Turkey: Çine Stream; c. C. meandrense, IFC-ESUF 03–1519, 183 mm SL, Turkey: Işıklı Spring; d. C. holmwoodii, IFC-ESUF 03–1513, 156 mm SL, Turkey: Gediz River; e. C. angorense, IFC-ESUF 03–1502, 152 mm SL, Turkey: Sakarya River; f. C. fahirae, IFC-ESUF 03–1512, 109 mm SL, Turkey: Dalaman River; g. C. beysehirense, IFC-ESUF 03–1505, 288 mm SL, Turkey: Beyşehir Lake.
See Figures (3) for general appearance, Table
Morphometric data of Chondrostoma smyrnae (holotype, IFC-ESUF 03-1566 and paratypes, IFC-ESUF 03-1567; n = 22).
Holotype | Holotype & paratypes | ||||
---|---|---|---|---|---|
mean | Min | Max | SD | ||
Standard length (mm) | 190.5 | 174.7 | 152.3 | 205.0 | |
In percent of standard length | |||||
Head length | 21.9 | 21.1 | 19.9 | 22.7 | 0.7 |
Body depth | 26.6 | 25.9 | 24.2 | 28.4 | 1.4 |
Body width at dorsal-fin | 17.5 | 17.4 | 16.0 | 19.4 | 1.2 |
Body width at caudal peduncle | 6.8 | 6.8 | 5.7 | 8.0 | 0.9 |
Predorsal distance | 51.2 | 50.8 | 49.0 | 52.2 | 1.2 |
Prepelvic distance | 52.6 | 53.1 | 51.1 | 54.6 | 1.8 |
Preanal distance | 71.4 | 73.2 | 70.4 | 75.7 | 2.6 |
Pectoral-fin origin to anal-fin | 49.6 | 52.8 | 49.7 | 58.7 | 1.7 |
Pectoral-fin origin to pelvic-fin | 29.8 | 32.0 | 29.8 | 36.2 | 1.5 |
Pelvic-fin origin to anal-fin | 19.6 | 22.1 | 19.8 | 24.6 | 0.9 |
Dorsal-fin depth | 18.4 | 18.4 | 17.1 | 20.6 | 0.8 |
Anal-fin depth | 16.2 | 15.4 | 14.4 | 16.9 | 0.9 |
Pectoral-fin length | 16.9 | 17.3 | 16.4 | 19.9 | 1.0 |
Pelvic-fin length | 15.9 | 14.9 | 11.3 | 17.3 | 0.9 |
Caudal peduncle length | 17.9 | 18.5 | 16.5 | 20.4 | 1.4 |
Caudal peduncle depth | 11.8 | 11.0 | 10.2 | 11.8 | 0.9 |
In percent of head length | |||||
Snout length | 31.6 | 33.1 | 31.1 | 35.3 | 1.2 |
Eye diameter | 18.5 | 20.7 | 17.5 | 22.8 | 1.4 |
Interorbital distance | 41.8 | 41.7 | 38.7 | 46.4 | 2.1 |
Head width1 | 56.9 | 59.7 | 54.9 | 65.3 | 2.3 |
Head depth1 | 54.7 | 54.6 | 50.9 | 57.8 | 2.4 |
Head depth2 | 81.1 | 79.9 | 75.0 | 85.2 | 2.6 |
Mouth width | 27.2 | 27.2 | 24.1 | 27.9 | 1.0 |
Morphometry of C. turnai (IFC-ESUF 03-1558. n = 43), C. meandrense (IFC-ESUF 03-1519. n = 24), C. holmwoodii (IFC-ESUF 03-1513. n = 19), and C. fahirae (IFCESUF 03-1512. n = 36).
C. turnai | C. meandrense | C. holmwoodii | C. fahirae | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SL (mm) | 132.0–191.8 | 120.6–200.9 | 88.8–161.3 | 82.8–96.8 | ||||||||||||
Mean | min | max | SD | mean | min | max | SD | mean | Min | max | SD | mean | min | max | SD | |
In percent of standard length | ||||||||||||||||
Head length | 21.9 | 20.7 | 23.4 | 0.8 | 22.6 | 21.9 | 23.6 | 0.5 | 23.1 | 21.5 | 24.0 | 0.4 | 24.1 | 23.3 | 25.1 | 0.6 |
Body depth | 24.6 | 22.9 | 27.0 | 1.0 | 23.9 | 21.8 | 25.5 | 1.0 | 23.6 | 22.0 | 24.8 | 0.7 | 24.0 | 21.9 | 25.3 | 1.1 |
Body width at dorsal-fin | 14.3 | 12.8 | 15.1 | 0.8 | 14.4 | 12.5 | 16.7 | 0.8 | 13.0 | 12.3 | 14.6 | 0.5 | 12.6 | 10.7 | 14.6 | 0.9 |
Body width at caudal peduncle | 5.3 | 4.0 | 5.8 | 1.4 | 5.00 | 3.5 | 5.9 | 0.9 | 5.6 | 4.7 | 6.6 | 0.7 | 5.6 | 4.1 | 6.9 | 1.2 |
Predorsal distance | 51.5 | 50.0 | 54.5 | 1.2 | 51.3 | 49.6 | 52.6 | 1.5 | 52.2 | 50.7 | 54.0 | 1.1 | 53.5 | 51.8 | 55.0 | 1.6 |
Prepelvic distance | 52.7 | 50.1 | 53.8 | 0.9 | 52.4 | 50.7 | 54.0 | 1.6 | 52.7 | 50.6 | 54.2 | 1.4 | 52.5 | 49.8 | 53.8 | 1.2 |
Preanal distance | 73.4 | 71.8 | 75.1 | 1.4 | 72.1 | 69.4 | 74.8 | 1.3 | 72.2 | 70.4 | 73.4 | 1.5 | 70.3 | 67.3 | 72.9 | 1.4 |
Pectoral-fin origin to anal-fin | 52.9 | 48.7 | 54.3 | 1.4 | 49.9 | 46.1 | 52.4 | 0.9 | 49.9 | 48.2 | 51.1 | 0.6 | 47.1 | 45.7 | 49.9 | 0.6 |
Pectoral-fin origin to pelvic-fin | 31.4 | 29.0 | 34.3 | 1.0 | 29.2 | 26.5 | 31.5 | 1.2 | 28.9 | 28.2 | 29.4 | 0.7 | 28.0 | 25.4 | 30.3 | 0.6 |
Pelvic-fin origin to anal-fin | 22.0 | 20.5 | 23.0 | 1.2 | 20.9 | 19.5 | 23.5 | 0.5 | 20.9 | 19.8 | 21.6 | 0.4 | 28.0 | 25.4 | 30.3 | 0.6 |
Dorsal-fin depth | 19.2 | 17.5 | 21.4 | 0.8 | 18.3 | 16.2 | 20.2 | 1.0 | 19.5 | 17.2 | 20.9 | 0.7 | 21.0 | 20.0 | 22.4 | 1.1 |
Anal-fin depth | 15.3 | 13.5 | 16.4 | 0.9 | 14.9 | 13.4 | 16.8 | 0.8 | 16.2 | 14.8 | 17.1 | 0.6 | 16.6 | 15.0 | 18.8 | 0.9 |
Pectoral-fin length | 18.0 | 16.0 | 20.1 | 0.8 | 17.5 | 15.4 | 19.1 | 0.9 | 18.4 | 18.0 | 19.4 | 0.9 | 20.5 | 18.7 | 21.9 | 0.9 |
Pelvic-fin length | 15.6 | 14.1 | 17.5 | 1.0 | 15.4 | 13.8 | 17.5 | 0.9 | 15.4 | 14.6 | 16.2 | 0.8 | 17.3 | 14.1 | 19.0 | 1.2 |
Caudal peduncle length | 17.4 | 14.5 | 18.5 | 0.5 | 18.2 | 16.7 | 20.3 | 0.9 | 17.8 | 16.2 | 19.4 | 0.6 | 19.4 | 18.2 | 20.9 | 0.9 |
Caudal peduncle depth | 10.5 | 9.70 | 11.7 | 0.8 | 10.5 | 9.9 | 11.2 | 0.4 | 10.2 | 9.4 | 10.9 | 0.2 | 10.8 | 9.9 | 11.9 | 0.5 |
In percent of head length | ||||||||||||||||
Snout length | 30.6 | 27.2 | 33.8 | 1.6 | 32.5 | 29.1 | 34.8 | 1.5 | 31.4 | 26.9 | 33.3 | 1.0 | 31.2 | 28.0 | 32.6 | 1.2 |
Eye diameter | 22.2 | 20.1 | 24.7 | 1.3 | 21.9 | 19.7 | 26.3 | 1.7 | 20.6 | 17.6 | 22.4 | 0.8 | 25.7 | 23.2 | 29.1 | 1.7 |
Interorbital distance | 38.2 | 33.1 | 41.9 | 2.1 | 36.4 | 33.7 | 38.7 | 1.4 | 36.1 | 32.7 | 39.0 | 1.1 | 37.8 | 35.1 | 39.6 | 1.6 |
Head width1 | 54.4 | 49.8 | 57.0 | 2.4 | 43.4 | 37.6 | 48.2 | 2.2 | 44.0 | 40.5 | 47.7 | 1.7 | 42.6 | 38.9 | 48.1 | 1.6 |
Head depth1 | 50.6 | 47.1 | 56.1 | 1.9 | 50.1 | 47.6 | 54.8 | 1.7 | 50.1 | 47.3 | 52.5 | 0.8 | 56.8 | 51.1 | 62.4 | 3.0 |
Head depth2 | 74.3 | 70.6 | 78.9 | 1.9 | 73.6 | 69.8 | 79.4 | 2.2 | 72.7 | 71.2 | 75.4 | 0.8 | 75.0 | 72.2 | 76.8 | 1.7 |
Mouth width | 26.1 | 23.1 | 27.7 | 1.3 | 25.8 | 23.8 | 28.3 | 1.1 | 27.4 | 25.3 | 29.3 | 1.4 | 25.4 | 24.4 | 27.8 | 1.1 |
The number of lateral line scales, scale rows between the lateral line and dorsal-fin origin, scale rows between the lateral line and pelvic-fin origin, branched dorsal-fin rays, branched anal-fin rays and rakers on the outer side of the first gill arch are shown in Tables
Frequency of lateral line scales, scale rows between the lateral line and dorsal-fin origin, and the lateral line and pelvic-fin origin in Eastern Aegean Chondrostoma species.
lateral line scales | |||||||||||||||||||||||||
N | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | Mean | |
C. smyrnae | 29 | 1 | 6 | 4 | 7 | 6 | 5 | 50.9 | |||||||||||||||||
C. turnai | 17 | 1 | 4 | 1 | 3 | 3 | 2 | 2 | 1 | 47.4 | |||||||||||||||
C. meandrense | 14 | 2 | 2 | 2 | 6 | 2 | 58.2 | ||||||||||||||||||
C. holmwoodii | 14 | 1 | 1 | 4 | 1 | 2 | 2 | 3 | 63.4 | ||||||||||||||||
C. fahirae | 24 | 1 | 1 | 5 | 6 | 5 | 3 | 1 | 1 | 1 | 47.6 | ||||||||||||||
scale rows between lateral line and dorsal-fin origin | scale rows between lateral line and pelvic-fin origin | ||||||||||||||||||||||||
N | 8 | 81/2 | 9 | 91/2 | 10 | 11 | Mean | 31/2 | 4 | 41/2 | 5 | 51/2 | 6 | 7 | Mean | ||||||||||
C. smyrnae | 28 | 19 | 9 | 8.3 | 28 | 4.0 | |||||||||||||||||||
C. turnai | 15 | 8 | 2 | 4 | 1 | 8.4 | 1 | 10 | 1 | 3 | 4.2 | ||||||||||||||
C. meandrense | 14 | 1 | 11 | 2 | 9.0 | 14 | 5.0 | ||||||||||||||||||
C. holmwoodii | 20 | 2 | 13 | 5 | 10.2 | 5 | 13 | 2 | 5.8 | ||||||||||||||||
C. fahirae | 10 | 5 | 5 | 8.5 | 7 | 3 | 4.3 |
Frequency of gill rakers, branched dorsal-fin rays, and branched anal-fin rays in Eastern Aegean Chondrostoma species.
Gill Rakers | |||||||||||||||||||
N | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | Mean | |
C. smyrnae | 16 | 3 | 5 | 3 | 3 | 2 | 20.8 | ||||||||||||
C. turnai | 11 | 1 | 2 | 4 | 3 | 1 | 24.2 | ||||||||||||
C. meandrense | 20 | 2 | 2 | 3 | 6 | 6 | 1 | 25.8 | |||||||||||
C. holmwoodii | 14 | 2 | 8 | 2 | 2 | 22.3 | |||||||||||||
C. fahirae | 7 | 1 | 3 | 3 | 13.3 | ||||||||||||||
Branched dorsal-fin rays | Branched anal-fin rays | ||||||||||||||||||
N | 7 | 8 | 9 | 10 | Mean | 8 | 9 | 10 | 11 | 12 | Mean | ||||||||
C. smyrnae | 36 | 2 | 34 | 7.9 | 2 | 5 | 29 | 9.8 | |||||||||||
C. turnai | 18 | 18 | 8.0 | 11 | 7 | 9.4 | |||||||||||||
C. meandrense | 14 | 12 | 2 | 8.1 | 8 | 6 | 9.4 | ||||||||||||
C. holmwoodii | 16 | 16 | 8.0 | 14 | 2 | 9.1 | |||||||||||||
C. fahirae | 10 | 10 | 7.0 | 3 | 7 | 8.7 |
In life: fins pinkish with hyaline margins; back brown; flank silvery with pinkish hue. After fixation: back and upper portion of flank dark greyish; mid-lateral portion of flank and belly yellowish. Dorsal and caudal fins dark grey, pelvic and anal fins yellowish. Peritoneal membrane black.
Chondrostoma smyrnae is known from the Tahtalı reservoir basin (Fig.
The species is named for Smyrna, the historic name of the city known today as Izmir. A noun in genitive, indeclinable.
The identification of Chondrostoma symrnae is a significant contribution to the genus Chondrostoma. There are six species of Chondrostoma (C. angorense, C. beysehirense, C. fahirae, C. holmwoodii, C. meandrense and C. turnai) in western Anatolia. The differences between Chondrostoma smyrnae, C. fahirae, C. holmwoodii, C. meandrense and C. turnai, which occur in the Aegean Sea basin of Turkey, are given in detail in the diagnosis section.
Chondrostoma smyrnae is distinguished from C. angorense and C. beysehirense, which occur adjacent to the Aegean Sea basin of Turkey, by having 48–53 lateral line scales (vs. 59–68 in C. angorense, 60–67 in C. beysehirense), 8–9 scale rows between the lateral line and the dorsal-fin origin (vs. 9–10 in C. angorense, vs. 10–11 in C. beysehirense), four scale rows between lateral line anal-fin origin (vs. 5 in C. angorense, 5–6 in C. beysehirense) and 19–23 gill rakers on first gill arch (vs. 33–39 in C. beysehirense).
In other words, their high genetic distance and position in the phylogenetic trees with high bootstrap values easily differentiated C. smyrnae from other species. The results on comparative morphological and genetic studies based on cyt b and coI genes showed that the new species differ from formerly described Chondrostoma species.
Material used for morphometric and meristic comparison
Chondrostoma angorense : IFC-ESUF 03-1501, 32, 80–174 mm SL; Turkey: Eskişehir prov.: Porsuk River about 2 km west of Yörükkırka, 39°36'00"N, 30°25'09"E. – IFC-ESUF 03-1502, 11, 35–162 mm SL; Turkey: Eskişehir prov.: stream Akin 0.5 km south of Akin, 39°20'02"N, 30°30'59"E. – IFC-ESUF 03-1503, 2, 245–300 mm SL; Turkey: Kütahya prov.: stream Emet about 10 km north of Eğriöz, 39°28'10"N, 29°15'17"E. – IFC-ESUF 03-1537, 23, 151–216 mm SL; Turkey: Kütahya prov.: stream Yanıkburnu about 25 km east of Dursunbey, 39°33'04"N, 28°56'55"E. – IFC-ESUF 03-1538, 3, 137–185 mm SL; Turkey: Balıkesir prov.: stream Bigadiç west of Bigadiç, 39°23'48"N, 28°04'50"E. – IFC-ESUF 03-1549, 1, 264 mm SL; Turkey: Afyonkarahisar prov.: stream Kali about 15 km west of Çay, 38°32'28"N, 30°50'41"E.
Chondrostoma beysehirense : IFC-ESUF 03-1505, 16, 156–251 mm SL; Turkey: Konya prov.: Beyşehir Lake about 20 km south of Şarkikaraağaç, 37°52'42"N, 31°20'46"E.
Chondrostoma fahirae : IFC-ESUF 03-1512, 36, 60–127 mm SL, Turkey: Burdur prov.: Başpınar Spring about 13 km south of Tefenni, 37°11'08"N, 29°45'16"E. – IFC-ESUF 03-1551, 1, 92 mm SL, Turkey: Burdur prov.: Dalaman River about 4 km north of Yusufça, 37°13'37"N, 29°32'57"E.
Chondrostoma holmwoodii : IFC-ESUF 03-1513, 19, 68–160 mm SL; Turkey: Manisa prov.: Gediz River at Derbent, 38°46'37"N, 29°12'41"E. – IFC-ESUF 03-1514, 7, 58–118 mm SL; Turkey: Manisa prov.: Gediz River about 16 km east of Kula, 38°35'46"N, 28°48'30"E. – IFC-ESUF 03-1515, 1, 112 mm SL; Turkey: Manisa prov.: Gediz River about 15 km north of Kula, 38°40'08"N, 28°36'14"E. – IFC-ESUF 03-1516, 1, 145 mm SL; Turkey: Manisa prov.: Gediz River about 5 km east of Gölmarmara, 38°42'08"N, 27°58'10"E. – IFC-ESUF 03-1517, 3, 85–102 mm SL; Turkey: İzmir prov.: Gediz River about 8 km east of Menemen, 38°37'42"N, 27°10'41"E.
Chondrostoma meandrense : IFC-ESUF 03-1519, 45, 120–209 mm SL, Turkey: Denizli prov.: Işıklı Spring, 38°19'19"N, 29°51'10"E. – IFC-ESUF 03-1522, 19, 96–151 mm SL, Turkey: Denizli prov.: streem Küfi about 4 km north of Işıklı, 38°21'48"N, 29°50'56"E. – IFC-ESUF 03-1523, 20, 110–219 mm SL, Turkey: Afyonkarahisar prov.: Stream Karasandıklı 0.5 km east of Karasandıklı, 38°31'40"N, 30°10'39"E. – IFC-ESUF 03-1525, 4, 65–138 mm SL, Turkey: Afyonkarahisar prov.: Suçıkan Spring 0.5 km east of Dinar, 38°04'14"N, 30°10'38"E. – IFC-ESUF 03-1561, 21, 50–158 mm SL, Turkey: Denizli prov.: Büyük Menderes River about 2 km west of Çıtak, 38°09'23"N, 29°38'24"E. – IFC-ESUF 03-1562, 3, 125–154 mm SL, Turkey: Denizli prov.: Büyük Menderes River about 1 km north of Hançalar, 38°07'54"N, 29°23'19"E.
Chondrostoma turnai : IFC-ESUF 03-1524, 44, 75–210 mm SL; Turkey: Aydın prov.: stream Çine about 8 km south of Aydın, 37°45'43"N, 27°50'12"E. – IFC-ESUF 03-1563, 1, 145 mm SL; Turkey: Denizli prov.: Cindere reservoir about 8 km south of Güney, 38°05'40"N, 29°01'32"E. – IFC-ESUF 03-1564, 3, 92–99 mm SL; Turkey: Denizli prov.: Yenicekent DSI Pomp about 3 km east of Yenicekent, 38°02'16"N, 28°57'47"E. – IFC-ESUF 03-1565, 15, 113–175 mm SL; Turkey: Aydın prov.: Akçay Stream about 3 km east of Sırma, 37°36'18"N, 28°29'34"E. – IFC-ESUF 03-1569, 1, 239 mm SL; Turkey: Denizli prov.: Vali Recep Yazıcıoğlu reservoir about 3 km east of Denizli, 37°46'14"N, 29°07'39"E.
Material used in molecular genetic analysis
Chondrostoma angorense : IFC-ESUF DNA-03-1502, 1; Turkey: Eskişehir prov.: stream Akin 0.5 km south of Akin, 39°20'02"N, 30°30'59"E (GenBank accession number: MT387066). – IFC-ESUF DNA-03-1501, 3; Turkey: Eskişehir prov.: Porsuk River about 2 km west of Yörükkırka, 39°36'00"N, 30°25'09"E (GenBank accession number: MT387112–MT387114). FFR DNA CH5-6, 2; Turkey: Kızılcahamam, Sakarya River drainage, (GenBank accession number: MW719604, MW719605). IFC-ESUF DNA-03-1500, 2; Turkey: Sivas prov.: Kızılırmak River about 3 km east of Zara, 39°54'27"N, 37°48'25"E (GenBank accession number: MW719606, MW719607). FFR DNA CH18-19, 2; Turkey: Yerköy, Delice stream, (GenBank accession number: MW719608, MW719609).
Chondrostoma beysehirense : IFC-ESUF DNA-03-1505, 2; Turkey: Konya prov.: Beyşehir Lake about 20 km south of Şarkikaraağaç, 37°52'42"N, 31°20'46"E (GenBank accession number: MT387079–MT387080). IFC-ESUF DNA-03-1505, 1; Turkey: Konya Prov.: Beyşehir, Beyşehir Lake, 37°55'43"N, 31°21'24"E (GenBank accession number: MW719597).
Chondrostoma fahirae : IFC-ESUF DNA-03-1512, 1; Turkey: Burdur prov.: Başpınar Spring about 13 km south of Tefenni, 37°11'08"N, 29°45'16"E (GenBank accession number: MT387128).
Chondrostoma holmwoodii : IFC-ESUF DNA-03-1513, 1; Turkey: Manisa prov.: Gediz River at Derbent, 38°46'37"N, 29°12'41"E (GenBank accession number: MT387099). – IFC-ESUF DNA-03-1516, 1; Turkey: Manisa prov.: Gediz River about 5 km east of Gölmarmara, 38°42'08"N, 27°58'10"E (GenBank accession number: MT387078). IFC-ESUF DNA-03-1513, 3; Turkey: Derbent Bridge, Manisa Prov.: Gediz River drainage, 38°46'40"N, 29°12'45"E (GenBank accession number: MW719598–MW719600).
Chondrostoma meandrense : IFC-ESUF DNA-03-1519, 4; Turkey: Denizli prov.: Işıklı Spring, 38°19'19"N, 29°51'10"E (GenBank accession number: MT387085–MT387088). IFC-ESUF DNA-03-1519, 2; Turkey: Işıklı Village, Denizli Prov.: Işıklı Spring, Büyük Menderes River drainage, 38°19'21"N, 29°51'07”E (GenBank accession number: MW719591–MW719592). IFC-ESUF DNA-03-1525, 3, Turkey: Afyonkarahisar prov.: Suçıkan Spring, Büyük Menderes River east of Dinar, 38°04'14"N, 30°10'38"E (GenBank accession number: MW719593–MW719595). FFR DNA CH13, 1; Turkey: Akçay, Büyük Menderes River drainage (GenBank accession number: MW719596).
Chondrostoma turnai : IFC-ESUF 03-1557, 5; Turkey: Aydın prov.: Stream Çine about 8 km south of Aydın, 37°45'43"N, 27°50'12"E. IFC-ESUF DNA-03-1524, 3; Turkey: Çiftlikburnu Village, Aydın Prov.: Çine Stream, Büyük Menderes River, 37°42'52"N, 27°50'04"E (GenBank accession number: MW719601–MW719602).
This study was supported by a grant from the Scientific and Technological Research Council of Turkey (TÜBİTAK) (Project No: KBAG-111T900). We are pleased to thank Müfit Özuluğ (İstanbul) for allowing Jörg Freyhof to participate in his many years of fieldwork. We thank Utku Avcı (Rize) for proofreading the manuscript and Esra Bayçelebi (Rize) for producing the distribution map (Fig.