Research Article |
Corresponding author: Shuxia Wang ( shxwang@nankai.edu.cn ) Academic editor: Michael Ohl
© 2018 Tengteng Liu, Encui Wang, Shuxia Wang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Liu T, Wang E, Wang S (2018) Taxonomic study of the leafmining genus Liocrobyla Meyrick, 1916 from China (Lepidoptera, Gracillariidae, Ornixolinae) with a description of one new species. Zoosystematics and Evolution 94(2): 305-314. https://doi.org/10.3897/zse.94.25460
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Three species of the leafmining genus Liocrobyla Meyrick, 1916 from China are treated. Liocrobyla indigofera sp. n. feeding on the plant genus Indigofera is described as new to science. Liocrobyla lobata Kuroko, 1960 feeding on Pueraria montana var. lobata is newly recorded in China. Lespedeza bicolor is documented as a new host plant for L. desmodiella Kuroko, 1982. Photographs of adult habitus, male and female genitalia, as well as host plants, leaf mines and biology are provided.
biology, distribution, host plant, new record, new taxon
The genus Liocrobyla Meyrick, 1916 belongs to the subfamily Ornixolinae Kuznetzov & Baryshnikova, 2001, which was confirmed by a recent phylogenetic study (
The purpose of this paper is to report one new species and one species of Liocrobyla recorded for the first time from China and to document a new host for the known species. All the species are provided with information on their host plant associations, leaf mines, and morphology of adults and their genital diagnostic characters.
Leaves containing mines with larvae were placed in sealed plastic bags or rearing containers with moist cotton as previously described (
Adult photographs were taken with a Leica S6D stereo microscope. Genitalia were dissected and mounted according to the methods introduced by
The types of the new species are deposited in the Zoological Collection, Shandong Normal University, Jinan, China (
TD Type depository
TL Type locality
Liocrobyla paraschista Meyrick: Kuroko, 1960: 2. Misidentification.
Liocrobyla
desmodiella
Kuroko, 1982: 185;
This species resembles Liocrobyla lobata Kuroko, 1960 in the general appearance of the forewing patterns, but can be distinguished by the blackish-grey forewing ground colour, which is brownish-grey in L. lobata. In the male genitalia, this species can be separated by the valva having a remarkable concavity at distal 1/4 and bearing a small ventro-apical hook; in L. lobata, the valva is almost parallel-sided and bears straight spines. The ninth tergite of the male L. desmodiella bears a pair of sclerotized lines originated from the middle of the posterior margin, which is absent in L. lobata.
China: Tianjin: 3♂, 1♀, Mt. Baxian, Ji County, 40.180°N, 117.550°E, 400 m, 2014.vi.24, leaf mines collected on Lespedeza bicolor, emerged 2014.vii.09, leg. Tengteng Liu, genitalia slide nos. LTT12611♂, LTT12612♀ (
Adult (Fig.
The forewing pattern of the specimens reared from Lespedeza bicolor is congruent to the Japanese specimens reared from L. cyrtobotrya (
Male genitalia (Figs
Female genitalia (Fig.
Biology (Figs
Host plants.Fabaceae: Lespedeza bicolor Turcz. in China, new record; L. cyrtobotrya Miq., Desmodium oldhamii Oliv., Ohwia caudata (Thunb.) H. Ohashi and Hylodesmum podocarpum subsp. oxyphyllum (DC.) H. Ohashi & R.R. Mill in Japan (
China: Tianjin (new record); Sichuan and Zhejiang (
The host plant Lespedeza bicolor was newly recorded for this species.
Liocrobyla
lobata
Kuroko, 1960: 5;
See Liocrobyla desmodiella for detail.
China: Shandong Province: 4♂, Mt. Kunyu National Nature Reserve, 37.292°N, 121.740°E, 400 m, Yantai City, 2017.vii.18, leaf mines collected on Pueraria montana var. lobata, emerged vii.29, leg. Tengteng Liu & Zhenquan Gao, genitalia slide nos. LIU0028, registered nos.
Adult (Fig.
Male genitalia (Figs
Biology (Figs
Fabaceae: Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep.
China: Shandong (new record), Japan (
Pueraria lobata has been treated as a synonym of Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep in
The new species resembles L. lobata in the male genitalia, but can be separated by the phallus longer than the valva and the minute cornutus; in L. lobata, the phallus is shorter than valva and the cornutus is more than 1/12 length of the phallus. In L. indigofera, the head is dark grey and the forewing ground colour is blackish fuscous, while in L. lobata, the head is white and the forewing ground colour is brownish grey.
Holotype, ♂, China: Shandong Province: Mt. Laoshan, 36.204°N, 120.609°E, 400 m, Qingdao City, 2017.vii.01, leafmine collected on Indigofera kirilowii, pupated vii.05, emerged vii.18, leg. Tengteng Liu & Zhenquan Gao, genitalia slide no. LIU0030♂, registered no.
Leaf mines collected on Indigofera tinctoria Linn., Mt. Laoshan, 120.609°E, 36.204°N, 400 m, Qingdao City, 2017.vii.01, leg. Tengteng Liu.
Adult (Figs
Male genitalia (Figs
Female genitalia (Fig.
Biology (Figs
Fabaceae: Indigofera kirilowii Palib., I. tinctoria L.
China (Shandong).
The specific name is derived from the genus name of the host plants.
Biology of Liocrobyla spp. 13–17. L. desmodiella, 13. host plant, Lespedeza bicolor; 14. leafmine; 15. frass opening on upper side of leaf; 16. frass opening on lower side of leaf; 17. cocoon and pupal exuviae; 18–20. L. lobata, 18. host plant, Pueraria montana var. lobata; 19. leafmines; 20. an opened mine with a larva exposed.
Host plants, leafmines and biology of Liocrobyla spp. 21–22, L. lobata, 21. leafmine with an alive larva; 22. frass opening on lower side of leaf; 23–28. L. indigofera sp. n., 23. leafmines each on a leaf of Indigofera kirilowii; 24–25. leafmines on I. tinctoria; 26. raceme of I. tinctoria; 27. leafmine with a living larva, identical mine to the lower-right mine in Fig.
Individual species of leaf miners, at least in many groups of Lepidoptera, are typically host-specific at a plant genus or family level (
Host plant specimens are recommended to be identified by a botany taxonomist and the nomenclature of the host plant is recommended to be checked in The Plant List which may be the most comprehensive single information resource covering all plants (
We are greatly indebted to the following colleagues and individuals: Luoyan Zhang and Xuejie Zhang, College of Life Sciences, Shandong Normal University, for identifying the host plants of Liocrobyla lobata and L. indigofera; Fuchen Shi, College of Life Sciences, Nankai University, for helping to identify the host plant of L. desmodiella; Bin Jiang, Mt. Kunyu National Nature Reserve, Yantai, for various help during the field work; Alexandra Rusakova, for translating the Russian description by