13urn:lsid:arphahub.com:pub:C9EFD5EB-E909-52A5-90B8-2C7119603A4Eurn:lsid:zoobank.org:pub:ED34F394-2E4C-49D6-8300-0DC18F233E6CZoosystematics and EvolutionZSE1435-19351860-0743Pensoft Publishers10.3897/zse.97.5796857968Research ArticleMicrohylidaeBiogeographyEvolutionary biologyPhylogenyTaxonomyCenozoicAsiaFar EastIndian Sub-Continent and HimalayasConsequences of parallel miniaturisation in Microhylinae (Anura, Microhylidae), with the description of a new genus of diminutive South East Asian frogsGorinVladislav A.https://orcid.org/0000-0002-9017-15021ScherzMark D.https://orcid.org/0000-0002-4613-776123KorostDmitriy V.4PoyarkovNikolay A.n.poyarkov@gmail.comhttps://orcid.org/0000-0002-7576-228315Faculty of Biology, Department of Vertebrate Zoology, Lomonosov Moscow State University, Leninskiye Gory 1/12, Moscow 119234, RussiaLomonosov Moscow State UniversityMoscowRussiaSektion Herpetologie, Zoologische Staatssammlung München (ZSM-SNSB), Münchhausenstr. 21, 81247, München, GermanyZoologische Staatssammlung MünchenMünchenGermanyInstitute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24–25, 14476 Potsdam, GermanyUniversity of PotsdamPotsdamGermanyGeological Faculty, Petroleum Geology Department, Moscow State University, Leninskiye Gory 1, Moscow 119234, RussiaMoscow State UniversityMoscowRussiaJoint Russian-Vietnamese Tropical Research and Technological Center, Nghia Do, Cau Giay, Hanoi, VietnamJoint Russian-Vietnamese Tropical Research and Technological CenterHanoiVietnam
Corresponding author: Nikolay A. Poyarkov (n.poyarkov@gmail.com)
Academic editor: Rafe Brown
2021120120219712154AD4AF59E-700D-59E9-BF5B-3E735B753126C6E9DCB9-E56D-48E1-A042-452A1D2043EE44516732508202014122020Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. PoyarkovThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://zoobank.org/C6E9DCB9-E56D-48E1-A042-452A1D2043EE
The genus Microhyla Tschudi, 1838 includes 52 species and is one of the most diverse genera of the family Microhylidae, being the most species-rich taxon of the Asian subfamily Microhylinae. The recent, rapid description of numerous new species of Microhyla with complex phylogenetic relationships has made the taxonomy of the group especially challenging. Several recent phylogenetic studies suggested paraphyly of Microhyla with respect to Glyphoglossus Günther, 1869, and revealed three major phylogenetic lineages of mid-Eocene origin within this assemblage. However, comprehensive works assessing morphological variation among and within these lineages are absent. In the present study we investigate the generic taxonomy of Microhyla–Glyphoglossus assemblage based on a new phylogeny including 57 species, comparative morphological analysis of skeletons from cleared-and-stained specimens for 23 species, and detailed descriptions of generalized osteology based on volume-rendered micro-CT scans for five species–altogether representing all major lineages within the group. The results confirm three highly divergent and well-supported clades that correspond with external and osteological morphological characteristics, as well as respective geographic distribution. Accordingly, acknowledging ancient divergence between these lineages and their significant morphological differentiation, we propose to consider these three lineages as distinct genera: Microhylasensu stricto, Glyphoglossus, and a newly described genus, Nanohylagen. nov.
Amphibiansintegrative taxonomynarrow-mouthed frogsmicro-computed tomographyNanohyla gen. novosteologysexual dimorphismtaxonomic revisionRussian Foundation of Basic Research (RFBR grant No. 19-34-90167)
oVert (openVertebrate) Thematic Collection Network (TCN) (NSF DBI-1701714; NSF DBI-1702263)Introduction
Anuran amphibians of the family Microhylidae (narrow-mouthed frogs) are globally distributed and diverse. This group currently comprises 12 subfamilies, 57 genera and over 700 recognized species, thus representing 10.3% of extant anuran diversity, making it the third largest anuran family after Hylidae and Strabomantidae (Streicher et al. 2020; Frost 2020). Microhylid frogs are morphologically and ecologically diverse including terrestrial, arboreal and fossorial (burrowing) morphotypes (Wells 2010; Moen et al. 2015). Microhylids display extensive variation in adult external morphology, osteology, and musculature; and in many cases, parallel specializations associated with a burrowing lifestyle may have led to remarkable morphological convergence (Emerson 1971; Wu 1994; Trueb et al. 2011; Moen et al. 2015). The extensive homoplasy observed in Microhylidae hinders phylogenetic interpretations from morphological characters (Wu 1994); significant progress in understanding evolutionary relationships of microhylids was achieved only through molecular phylogenetic studies (de Sá et al. 2012, 2019a, 2019b; Tu et al. 2018; Streicher et al. 2020). Skeletal composition varies substantially among microhylids, showing features that are rare or absent in other anuran clades (Noble and Parker 1926; Parker 1934; Zweifel 1972; 1986; de Sá and Trueb 1991). Although osteology and morphological evolution of microhylid subfamilies from the Americas (de Sá and Trueb 1991; Lehr and Trueb 2007; Trueb et al. 2011; Zweifel 1986, and references therein), Australasia (Zweifel 1972), and Madagascar (Scherz et al. 2015, 2016, 2017, 2019) received a decent amount of researchers’ attention, studies of the Asian microhylid subfamilies, to date, have primarily focused on description of long-neglected species diversity (Matsui et al. 2011; Hasan et al. 2014; Poyarkov et al. 2014, 2018a, 2018b, 2019, 2020; Vassilieva et al. 2014; Yuan et al. 2016; Suwannapoom et al. 2018, 2020; Nguyen et al. 2019, and citations therein).
The first and only monographic revision of the family Microhylidae published over 85 years ago was largely based on osteological data (Parker 1934). In his review of Asian microhylid taxa, Parker only focused on the most variable parts of the skeleton (such as the palatine region and pectoral girdle), but description of generalized osteology generally was not included (Parker 1934). In recent years skeletal morphology of only a few species in Microhylinae has been described in substantial detail, including the genus Uperodon (Chandramouli and Dutta 2015; Garg et al. 2018), Kaloulaborealis (Boring and Liu 1937; Zhang et al. 2020), and Glyphoglossusguttulatus (McPartlin 2010).
The genus Microhyla Tschudi, 1838 currently comprises 52 nominal species (Hoang et al. 2020; Poyarkov et al. 2020a, 2020b; Frost 2020) and several undescribed candidate species (Gorin et al. 2020). It is the second largest microhylid genus after Oreophryne (Frost 2020) and the most species species-rich taxon of the Asian subfamily Microhylinae. Over half of Microhyla species diversity was described within the last 15 years (29 species, see Frost 2020), but despite substantial progress in their taxonomy, this genus remains one of the most taxonomically challenging groups of Asian frogs. The small or medium-sized terrestrial frogs of the genus Microhyla are distributed all over the Oriental biogeographic region (Fig. 1) and exhibit significant variation in body size (adult body size varies from 10–46 mm) and ecomorphology (e.g. body shape, finger and toe disc expansion, and limb lengths) tied to their natural history (terrestrial, semi-arboreal, semi-fossorial). The smallest Microhyla species are amongst the smallest frogs in the world, approaching the lower body-size limit for the vertebrate bauplan (Das and Haas 2010; Kraus 2011). Phylogenetic analyses based on molecular data (Matsui et al. 2011; Garg et al. 2019; Gorin et al. 2020), provided novel insights into phylogeny of the genus and revealed significant inconsistencies with the traditional, morphology-based classifications (Parker 1934; Dubois 1987; Fei et al. 2009). The preliminary mitochondrial DNA (mtDNA) based genealogies unexpectedly suggested paraphyly of Microhyla with respect to the large-sized fossorial genus Glyphoglossus (Matsui et al. 2011; de Sá et al. 2012; Biju et al. 2019; Nguyen et al. 2019; Poyarkov et al. 2019). Additional multilocus phylogenetic (Garg and Biju 2019; Gorin et al. 2020) and phylogenomic (Tu et al. 2018; Peloso et al. 2016) studies supported monophyly of Microhyla, and agreed with one another in recovering the three main highly-divergent lineages within this group: the Glyphoglossus clade and two Microhyla clades (Microhyla I and Microhyla II hereafter, following Gorin et al. 2020). The three major clades of the Microhyla–Glyphoglossus assemblage were shown to have diversified in the middle Eocene (Garg and Biju 2019; Gorin et al. 2020), which makes the genus Microhyla, sensu lato (hereafter s. lat.) older than other microhyline genera (Feng et al. 2017; Garg and Biju 2019). The lack of information on morphological variation among and within the lineages of the Microhyla–Glyphoglossus assemblage hinders further taxonomic assessment of diversity within this group.
Distribution ranges of the three clades of the Microhyla–Glyphoglossus assemblage. Distribution area of Microhyla I is shown in yellow, of Microhyla II in red, and of Glyphoglossus in blue. Distributional data from Gorin et al. (2020). Question mark denotes the unconfirmed record of “Microhylaannamensis” from Khao Sebab in eastern Thailand by Taylor (1962).
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Herein, we assess the status of the three lineages of the Microhyla–Glyphoglossus assemblage using an integrative taxonomic approach. We provide an updated mtDNA-based genealogy including 57 species of the group. Based on traditional (cleared-and-stained specimens and external morphology) and digital (micro-Computed Tomography, or micro-CT) methods of comparative morphology we further report on osteological variation for 23 species of the genus Microhyla and three species of the genus Glyphoglossus, thus covering all major lineages for the first time. Based on analysis of morphological, osteological, molecular, and distribution data we recognize Glyphoglossus and Microhyla I sensu stricto (hereafter as s. str.) as valid genera. Additionally, we erect a new genus for Microhyla II, helping to stabilize the taxonomy of this clade. We further analyze miniaturization patterns, body size, and the evolution of sexual dimorphism in the Microhyla–Glyphoglossus assemblage.
Material and methodsTaxon sampling and examined specimens
To assess the phylogenetic relationships within the Microhyla–Glyphoglossus assemblage we used the mtDNA and nuclear DNA (nuDNA) datasets from Gorin et al. (2020) with the addition of sequences of the recently described Mysticellusfranki (Garg and Biju 2019) and Microhylahongiaoensis (Hoang et al. 2020). We used the mtDNA dataset, consisting of 12S rRNA and 16S rRNA for all examined samples, for estimation of the phylogeny (232 sequences, including 200 sequences of Microhyla). A combined mtDNA + nuDNA dataset, joining the long 12S rRNA–16S rRNA mtDNA fragment and BDNF gene sequences for a reduced set of 120 samples, equitably selected (from preliminary analysis of mtDNA; not shown) to represent all major lineages within Microhyla, was used to estimate a robust, multilocus, time-calibrated phylogeny. In total, we analyzed GenBank sequences from 200 specimens of 49 nominal and three candidate Microhyla species, five species of Glyphoglossus, and 32 other microhylids, including representatives of all currently recognized microhyline genera. All taxa, specimen-associated locality data, museum voucher catalog numbers, and genetic data included in our study are presented in Suppl. material 1: Table S1.
Our osteological study was based on specimens housed in herpetological collections of the Zoological Museum of Lomonosov Moscow State University (ZMMU, Moscow, Russia), the Herpetology Lab of the Vertebrate Zoology department, Faculty of Biology, Lomonosov Moscow State University (HLMU; Moscow, Russia), the Museum of Comparative Zoology, Harvard University (MCZ, Cambridge, Massachusetts, USA), and the California Academy of Sciences (CAS, San Francisco, California, USA). Altogether, for hand-preparation and histological clearing-and-staining, we used 23 specimens, which included 17 nominal species of Microhyla I clade, and 4 species of Microhyla II clade, and representing all of the currently recognized species groups, with exception of the M.palmipes species group, and two species of Glyphoglossus. All specimens were adults, fixed in either 75% ethanol or in 4% buffered formalin with subsequent storage in 70% ethanol. Additionally, for micro-computed tomography (micro-CT) study we examined the smallest representatives of Microhyla I and Microhyla II clades (M.nepenthicola and M.arboricola, respectively). We also used micro-CT scans for one Microhyla (M.achatina, the type species of the genus; MCZ-A2683, ark:/87602/m4/M79961) and two species of Glyphoglossus: G.yunnanensis (CAS-H-242243, ark:/87602/m4/M49927) and G.molossus (the type species of the genus; CAS-H-243121, ark:/87602/m4/M49928), downloaded from the MorphoSource database (www.morphosource.org) with permission. Altogether, our morphological dataset included detailed information for 23 species of Microhyla and 3 species of Glyphoglossus. Detailed information on the species and specimens included in morphological study is presented in Suppl. material 2: Table S2.
Phylogenetic inference
Nucleotide sequences were initially aligned in MAFFT v.6 (Katoh et al. 2002) with default parameters, and were subsequently manually optimized in BioEdit 7.0.5.2 (Hall 1999). Genetic distances were calculated using MEGA 6.1 (Tamura et al. 2013). The optimal partitioning schemes for our alignment were identified with PartitionFinder 2.1.1 (Lanfear et al. 2012) using the greedy search algorithm under AICc criterion. Phylogenetic trees were reconstructed under maximum likelihood (ML) and Bayesian inference (BI). A ML analysis was implemented using the IQ-TREE webserver (Nguyen et al. 2015; Trifinopoulos et al. 2016). Clade stability was assessed by 1000 bootstrap (BS) replications and expected likelihood weights (ELW). One-thousand bootstrap pseudoreplicates (MLBS) were employed, and nodes having MLBS values of 90 and above were considered strongly supported, while nodes with values of 75–90 were regarded as significantly supported, lower values were considered to indicate lack of nodal support (Felsenstein 1985; Huelsenbeck and Hillis 1993).
Bayesian inference (BI) was performed in MrBayes v3.1.2 (Ronquist and Huelsenbeck 2003). Metropolis-coupled Markov chain Monte Carlo (MCMCMC) analyses were run with one cold chain and three heated chains for one million generations, with sampling every 100 generations. We performed five independent MCMCMC runs and the initial 10% of trees were discarded as burn-in. We checked that the effective sample sizes (ESS) were all above 200 by exploring the likelihood plots using TRACER v1.6 (Rambaut et al. 2014). We assessed the clade support with posterior probabilities (PP) (Huelsenbeck and Ronquist 2001). Nodes with PP of 0.95 and above were considered strongly supported, nodes with values of 0.90–0.94 as significantly supported, while lower values were considered as no support (Huelsenbeck and Ronquist 2001; Wilcox et al. 2002). Molecular divergence time estimation was performed in BEAST v1.8.4 (Drummond et al. 2012). Molecular clock assumptions were tested using hierarchical likelihood ratio tests in PAML v4.7 (Yang 2007), which suggested the use of uncorrelated lognormal relaxed clock for our dataset. The models and partitioning scheme from our ML analysis were also incorporated into these subsequent divergence date estimations; we set the Yule model as the tree prior, assumed a constant population size, and used default priors for all other parameters. In BEAST, we conducted two runs of 200 million generations each, sampled every 4000 steps, parameter convergence was estimated in Tracer, and the first 10% of generations discarded as burn-in. TreeAnnotator v1.8.0 (in BEAST) was used to create our maximum clade credibility tree from the remaining samples. Calibration priors and all other details of this analysis followed Gorin et al. (2020).
Osteological preparation and double staining
In order to observe both ossified and cartilaginous structures, specimens were cleared and double stained with alcian blue for cartilage and alizarin red for bone. We used the most delicate methodology of acid-free staining (following Walker and Kimmel 2006) to preserve minute skeletal elements of the smallest species. The protocol included: (1) staining for about 24 hours in a solution of 0.05% alizarin red, 0.02% alcian blue, 45mM MgCl2 and 70% ethanol; (2) maceration for about 24 hours at 37 °C in a saturated solution of sodium tetraborate with 1% trypsin; (3) bleaching for several hours in a solution of 1.5% H2O2 and 1% KOH; (4) clearing with successive changes of solutions of 25/50/75% glycerol with 0.25% KOH, for 1/3/5 days for each solution respectively; and final (5) storage in a 99% glycerol. Obtained skeletons were examined and photographed using a LEICA EZ4 dissecting stereo microscope (Leica Camera AG, Wetzlar, Germany) with a binocular-implemented ES-ESPERTS Digital camera BR-5101LC-UF.
Micro-CT scanning
We followed Micro-CT scanning of M.nepenthicola (ZMMU A-6028-1) and M.arboricola (ZMMU A-5051), using protocols of Suwannapoom et al. (2018) and Poyarkov et al. (2018). Scanning was conducted at the Petroleum Geology Department, Faculty of Geology, Lomonosov Moscow State University, using a SkyScan 1 172 desktop scanner (Bruker micro-CT, Kontich, Belgium) equipped with a Hamamatsu 10 Mp digital camera. Both specimens were mounted on a polystyrene baseplate and placed inside a hermetically sealed polyethylene vessel. Scans were conducted with a resolution of 3.7 μm at 40 kV voltage and a current of 250 mA, with a rotation step of 0.3°. We used oversize mode, in which three blocks of sub-scan data were connected vertically, to obtain a general tomogram. We used 3D Slicer (Kikinis et al. 2014) for construction and processing of 3D-models. Scans were deposited in MorphoSource (http://www.morphosource.org/Detail/ProjectDetail/Show/project_id/1183).
Morphological descriptions and analyses
Osteological terminology followed Trueb (1968, 1973), Scherz et al. (2017), Suwannapoom et al. (2018), and Poyarkov et al. (2014, 2018a). Terminologies used to describe the shape of terminal phalanges (simple, knobbed, T-shaped, and Y-shaped) followed Parker (1927) and Garg et al. (2019). Comparative morphological and osteological data for other genera were taken from a number of revisions of Microhylinae (Parker 1934; Boring and Liu 1937; Duellman and Trueb 1986; Dubois 1987; Fei et al. 2009; McPartlin 2010; Chandramouli and Dutta 2015; Garg et al. 2019; Garg and Biju 2019; Poyarkov et al. 2018b; Zhang et al. 2020; Suwannapoom et al. 2020). External morphology was described following Poyarkov et al. (2014, 2019); mensural data were taken with a Mitutoyo dial caliper (Mitutoyo Corporation, Kawasaki, Japan) to the nearest 0.1 mm. We recorded the following external morphology characters: snout-vent length (SVL, measured as distance from tip of snout to cloaca), body shape (slender, stocky, or stout, following Bain and Nguyen 2004), snout profile (in lateral and dorsal view), dorsal skin texture (smooth, shagreened, feebly granular or tuberculate), relative length of first finger (FI length: ≤ 1/2 of FII length, ≥ 1/2 of FII length, or reduced to a nub), widths of discs on fingers and toes, number and shape of metatarsal tubercles, the presence (vs absence) of dorsomedial grooves on fingers and toes, of a distinct dorsomedial (vertebral) line, of superciliary tubercles, and of externally visible tympanum, the level to which the tibiotarsal articulation of an adpressed leg reaches (not reaching the eye, to the eye, to the snout, far beyond the snout), and the development of toe webbing (rudimentary, basal, well-developed, developed to discs; webbing and subarticular tubercle formulas follow Savage 1975).
To assess body size and sexual dimorphism evolution in the Microhyla–Glyphoglossus assemblage, we compiled data on maximum snout-vent length (SVL) separately for both sexes, for each species reported in literature and/or from our own measurements of voucher specimens following Gorin et al. (2020). Size (SVL) data for all Microhyla and Glyphoglossus species are summarized in Suppl. material 3: Table S3. Comparative morphological analyses were conducted in R 3.6.3 (R Core Team 2014). Analyses of SVL measurements were carried out using their natural logarithms. Sexual dimorphism was expressed as a ratio of male to female SVL (female-biased species have > 1, male-biased species have < 1). The tree and morphological dataset were pruned to reflect taxa represented in both, using the treedata() function in geiger (Harmon et al. 2008). Continuous trait evolution was mapped to the phylogeny using the contMap() function of phytools (Revell 2012). Phylogenetic Least Squares (PGLS) analysis of the log of male SVL against dimorphism was carried out using caper package (Orme et al. 2018) and plotted with ggplot2 (Wickham 2016). Species were binned into four size categories (terminology follows Scherz et al. 2019) as follows: ≤ 13 mm (state 1: “extremely miniaturized”); (2: 13–16 mm, “highly miniaturized”); (3: 16–20 “miniaturized”); (4: 20–24 “small”).
ResultsPhylogenetic relationships
Our final aligned matrix of mtDNA data contained 232 sequences (length 2478 bp), representing 49 of the 52 currently recognized species of the genus Microhylas. lat., three undescribed candidate species of Microhyla, and five species of Glyphoglossus. Our final alignment of the nuDNABDNF gene was 720 bp long, and included all of the taxa sampled for the mitochondrial matrix but for six Microhylas. lat. species (from clade I: M.gadjahmadai, M.taraiensis, M.mixtura, M.fanjingshanensis, and M.beilunensis; from clade II: M.perparva). We here report on mitochondrial-only and nuclear-only phylogenies first, and concatenated phylogenies afterwards.
Both BI and ML phylogenetic methods resulted in identical topology of mtDNA-based genealogical relationships for the Microhyla–Glyphoglossus assemblage (Fig. 2). All analyses concordantly resolved three strongly supported major clades within the group: Microhyla I, Microhyla II, and Glyphoglossus, as indicated by Bayesian posterior probabilities of 1.0 and ML bootstrap node support of 100% (node support values are hereafter provided as PP/BS); the majority of ingroup nodes also received strong support (PP/BS ≥ 0.95/95%). Although the Microhyla–Glyphoglossus assemblage was recovered to be a monophyletic group with strong support (1.0/100), the relationships among the three main clades within it remained essentially unresolved according to the mtDNA dataset, and the grouping of Microhyla I + Glyphoglossus received no nodal support (-/70) (Fig. 2; Suppl. material 6: Figure S1A and Suppl. material 6: Figure S2). Phylogenetic analyses of the nuDNABDNF gene suggested monophyly of Mirohyla I + Microhyla II grouping with moderate to strong node support (0.90/97; Suppl. material 6: Figure S1B), despite the short length of this marker. Relationships at shallower nodes within the respective clades were less strongly resolved than in the mtDNA phylogeny. The combined mtDNA + nuDNA analyses (3207 bp) yielded a topology largely congruent with that of the nuDNA alone, but with lower node support values for the Mirohyla I + Microhyla II clade (0.46/90; Fig. 3; detailed in Suppl. material 6: Figure S1C). Thus, while the three major clades in the Microhyla–Glyphoglossus assemblage are strongly and consistently recovered as monophyletic, the monophyly of Microhylas. lat. remains tentative, with practically no signal in the mitochondrial dataset but some signal in BDNF. As the combined dataset yielded a better resolved phylogeny that is also more consistent with previous work (e.g. Tu et al. 2018), we use that tree for further analyses (time tree, ancestral state reconstruction) and discussion below.
Diversity of the Microhyla–Glyphoglossus assemblage based on an updated mtDNA-genealogy derived from the analysis of 2478 bp of alignment including 12S rRNA, tRNAVal, 16S rRNA gene fragments. Black circles correspond to well-supported (PP ≥ 0.95; BS ≥ 90) and white circles to moderately supported (0.95 > PP ≥ 0.90; 90 > BS ≥ 75) nodes; no circles indicate unsupported nodes. Letters A–I denote the species groups of Gorin et al. (2020). Photos by Nikolay A. Poyarkov, Indraneil Das, Vladislav A. Gorin, Parinya Pawangkhanant, Luan Thanh Nguyen, and Evgeniya N. Solovyeva. For full version of this tree showing the outgroups and node support values see Suppl. material 7: Figure S2.
Bayesian inference tree of the Microhyla–Glyphoglossus assemblage derived from the combined mtDNA + nuDNA analysis of 3207 bp of alignment including 12S rRNA, tRNAVal, 16S rRNA and BDNF gene fragments. Black circles correspond to well-supported (PP ≥ 0.95; BS ≥ 90) and white circles to moderately supported (0.95 > PP ≥ 0.90; 90 > BS ≥ 75) nodes; no circles indicate unsupported nodes. Letters A–I denote the species groups of Gorin et al. (2020). For voucher specimen information and GenBank accession numbers see Suppl. material 1: Table S1. Yellow, red, and blue color denotes Microhyla I, Microhyla II, and Glyphoglossus, respectively. Numbers at tree nodes correspond to PP/BS support values, respectively (shown only for moderately supported nodes). For full version of this tree showing the outgroups and node support values see Suppl. material 6: Figure S1.
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The observed topological patterns within the Microhyla–Glyphoglossus assemblage were congruent with earlier results of Gorin et al. (2020) in recovering eight major species groups within Microhylas. lat. (clades A–H, see Figs 2–3), and the genus Glyphoglossus (clade I, see Figs 2–3). The only difference with results of Gorin et al. (2020) is the phylogenetic placement of the recently described M.hongiaoensis as sister species to M.pulchella (Figs 2–3). Since a detailed description of phylogenetic relationships within the genus Microhyla was provided by Gorin et al. (2020), we only focus here on a general description of the most important basal nodes, crucial for discussion in the present study.
The most species-rich clade, Microhyla I, is widely distributed from mainland southern China, Hainan and Taiwan, and the Ryukyu Archipelago of Japan in the north, through the Indochina Peninsula, to India, and Sri Lanka in the west, and through the Malayan Peninsula to Borneo, Sumatra, Java and Bali in the south (Fig. 1). The Microhyla I clade contained the following species, clustered in seven species groups (Fig. 2): The Microhylaachatina group (clade A), including M.achatina Tschudi, 1838; M.borneensis Parker, 1928; M.fodiens Poyarkov, Gorin, Zaw, Kretova, Gogoleva, Pawangkhanant & Che, 2019; M.gadjahmadai Atmaja, Hamidy, Arisuryanti, Matsui & Smith, 2018; M.heymonsi Vogt, 1911; M.irrawaddy Poyarkov, Gorin, Zaw, Kretova, Gogoleva, Pawangkhanant & Che, 2019; M.kodial Vineeth, Radhakrishna, Godwin, Anwesha, Rajashekhar & Aravind, 2018; M.malang Matsui, 2011; M.mantheyi Das, Yaakob & Sukumaran, 2007; M.minuta Poyarkov, Vassilieva, Orlov, Galoyan, Tran, Le, Kretova & Geissler, 2014; M.nepenthicola Das & Haas, 2010; M.orientalis Matsui, Hamidy & Eto, 2013; M.pineticola Poyarkov, Vassilieva, Orlov, Galoyan, Tran, Le, Kretova & Geissler, 2014; and two undescribed candidate species, Microhyla sp. 1 and Microhyla sp. 3.
The Microhylafissipes group (clade B), including M.beilunensis Zhang, Fei, Ye, Wang, Wang & Jiang, 2018; M.chakrapanii Pillai, 1977; M.fanjingshanensis Li, Zhang, Xu, Lv & Jiang, 2019; M.fissipes Boulenger, 1884; M.mixtura Liu & Hu, 1966 in Hu et al. (1966); M.mukhlesuri Hasan, Islam, Kuramoto, Kurabayashi & Sumida, 2014; M.mymensinghensis Hasan, Islam, Kuramoto, Kurabayashi & Sumida, 2014; M.okinavensis Stejneger, 1901; and an undescribed candidate species, Microhyla sp. 2.
The Microhylaberdmorei group (clade C), including M.berdmorei (Blyth, 1856); M.picta Schenkel, 1901; and M.pulchra (Hallowell, 1861).
The Microhylaornata group (clade E), including M.mihintalei Wijayathilaka, Garg, Senevirathne, Karunarathna, Biju & Meegaskumbura, 2016; M.nilphamariensis Howlader, Nair, Gopalan & Merilä, 2015; M.ornata (Duméril & Bibron, 1841); M.rubra (Jerdon, 1854); and M.taraiensis Khatiwada, Shu, Wang, Thapa, Wang & Jiang, 2017.
The Microhylabutleri group (clade F), including M.aurantiventris Nguyen, Poyarkov, Nguyen, Nguyen, Tran, Gorin, Murphy & Nguyen, 2019; and M.butleri Boulenger, 1900.
The Microhylapalmipes group (clade G), including M.palmipes Boulenger, 1897. The distribution area of the Microhyla II clade is restricted to the montane forest areas in the Annamite (Truong Son) Mountains in East Indochina (Vietnam, eastern Laos, northeastern Cambodia), Malayan Peninsula (Titiwangsa Mountain Range), mountains of Borneo (Sarawak, Sabah of Malaysia, Brunei and northern Kalimantan, Indonesia), and the southwestern-most islands of the Sulu Archipelago of the Philippines (Fig. 1). It contains the following nine species (clade H, Fig. 2) of the M.annectens group: M.annamensis Smith, 1923; M.annectens Boulenger, 1900; M.arboricola Poyarkov, Vassilieva, Orlov, Galoyan, Tran, Le, Kretova & Geissler, 2014; M.hongiaoensis Hoang, Nguyen, Luong, Nguyen, Orlov, Chen, Wang & Jiang, 2020; M.marmorata Bain & Nguyen, 2004; M.nanapollexa Bain & Nguyen, 2004; M.perparva Inger & Frogner, 1979; M.petrigena Inger & Frogner, 1979; and M.pulchella Poyarkov, Vassilieva, Orlov, Galoyan, Tran, Le, Kretova & Geissler, 2014.
Finally, the Glyphoglossus clade (clade I, Fig. 2) covers the whole Thai-Malaysian Peninsula, parts of Indochina, including Myanmar, and also penetrates northward, as far as southern mainland China; and southward as far as Sumatra and Borneo (Fig. 1). It contains the following five species: G.capsus (Das, Min, Hsu, Hertwig & Haas, 2014); G.guttulatus (Blyth, 1856); G.minutus (Das, Yaakob & Lim, 2004); G.molossus Günther, 1869; and G.yunnanensis (Boulenger, 1919).
Divergence times
Estimated node-ages (mean age estimate ± 95% highest posterior density interval [95% HPD]) for main nodes are detailed in Suppl. material 4: Table S4 and Suppl. material 8: Figure S3. The results of the divergence time estimation fully agree with Gorin et al. (2020), suggesting that the most recent common ancestor (MRCA) of Microhyla and Glyphoglossus originated around the early Eocene ca. 50.9 million years ago (hereafter Ma, 95% HPD 44.2–58.7) (Suppl. material 8: Figure S3). This estimate coincides with some previous estimates (48.8 Ma; 45.9–53.2, Feng et al. 2017), but is significantly younger than other reports (61.5, 56.6–66.5, Garg and Biju 2019). The Microhyla–Glyphoglossus assemblage radiated into the three major clades in the middle Eocene (44.1, 38.5–49.6), notably later than other estimates (48.7, 44.1–53.2, Garg and Biju 2019). Subsequent diversification of each genus-level radiations of Microhyla I, Microhyla II, and Glyphoglossus clades initiated much later in the early to middle Oligocene (ca. 35–25 Ma, Gorin et al. 2020).
Comparative osteology
A total of 26 species examined for osteological variation allows us to clarify similarities and variation in skeletal morphology among and within the three clades of the Microhyla–Glyphoglossus assemblage. Detailed information on species’ characters’ states is presented in Suppl. material 5: Table S5. Overall skeletal morphology and the main osteological features for representatives of each clade are illustrated in Figures 4–7. Skull and hand morphology for cleared and stained representatives of these three clades is provided in Suppl. material 8, 9.
Below, we provide comparative osteological descriptions for the three clades of the Microhyla–Glyphoglossus assemblage: Microhyla I, Microhyla II, and Glyphoglossus.
(A) Microhyla I clade
This clade includes M.achatina, the type species of the genus Microhyla, and is the most widely distributed, species rich, and ecologically and morphologically diverse group of the Microhyla–Glyphoglossus assemblage Clade I includes most small- to medium-sized terrestrial species, along with several large species; they are adapted to fossorial (M.picta), or semi-fossorial (M.fodiens, M.rubra, M.mihintalei) lifestyles (Fig. 2). This diversity is also reflected in osteological features, which demonstrate conspicuous variation among species (Fig. 4; Suppl. material 5: Table S5). Due to marked morphological variation, providing a comprehensive morphological diagnosis of this speciose group remains a challenging task; below we summarize available information on skeletal traits.
General osteology of the Microhyla–Glyphoglossus assemblage representatives. The full skeletons are shown for Glyphoglossusmolossus (A – dorsal, B – ventral views), Glyphoglossusyunnanensis (C – dorsal, D – ventral views), Microhylaachatina (E – dorsal, F – ventral views), Microhylanepenthicola (G – dorsal, H – ventral views), and Nanohylaarboricola (I – dorsal, J – ventral views). Note: figures display only calcified structures; cartilages are omitted due to limitations of micro-CT scanning. Scale bar equals 5 mm.
https://binary.pensoft.net/fig/496418
Skull
Skull longer than wide, wider than long, or almost in equal proportions among species of Microhyla I (Fig. 5; Suppl. material 5: Table S5). Widest part of skull located posteriorly and giving head a triangular or trapezoid shape (Fig. 5). Frontoparietals longer than broad, narrowing anteriorly, in contact along medial border but not fused, lacking any dorsal crests; partially fused with or separated from exoccipital posteriorly and prootic posterolaterally. Exoccipitals always separate, in contact medially. Nasals large and widely separated, chondrified peripherally; processus paraorbitalis broad, in some species blunt, posterior edge concave, anterior edge convex (Fig. 5). Sphenethmoid well-ossified, always clearly separated from parasphenoid, with a concave posterior edge. Prootics ossified anteromedially; crista parotica cartilaginous with posterior margin mineralized. Squamosal ossified, with a well-developed ventral ramus and poorly developed otic and zygomatic rami. Operculum slightly mineralized. Majority of columella (stapes) mineralized, with only pars externa plectra cartilaginous; tympanic annulus completely chondrified (Suppl. material 10: Figure S5H). Premaxilla well-ossified, its alary process oriented slightly anteriorly, distal part bending laterally. Maxilla well-ossified; anteriorly in contact with labial portion of premaxilla (eleutherognathine condition); edentate; pars facialis moderately high in lateral view. Quadratojugal reduced, with a chondrified posterior articulation with angulosplenial; not in anterior contact with maxilla. Support of upper jaw taken over by pterygoid, with long anterior ramus, broad posterior ramus, and short medial ramus. Vomers small, widely separated, triangular in shape. Neopalatines present or absent. Nasal capsules mineralized posteriorly or entirely cartilaginous. Mentomeckelians ossified, connected to dentaries and to each other through Meckel’s cartilage. Dentary fused with angulosplenial. Parasphenoid smooth; cultriform process of parasphenoid narrowing anteriorly, terminating at level of sphenethmoid with a chondrified notch (Fig. 5). Hyoid plate completely cartilaginous, anterolateral (alary) processes of hyoid plate present, recurved, posterolateral processes slender, posteromedial processes strongly ossified, elongated, straight, wider at proximal ends, chondrified at distal ends, separated by a chondrified parahyoid (Suppl. material 10: Figure S5M).
Cranial osteology of the Microhyla–Glyphoglossus assemblage representatives. The skulls are shown in dorsal / ventral / lateral views for Glyphoglossusmolossus (A / B / C, respectively), Glyphoglossusyunnanensis (D / E / F, respectively), Microhylaachatina (G / H / I, respectively), Microhylanepenthicola (J / K / L, respectively), and Nanohylaarboricola (M / N / O, respectively). Note: figures display only calcified structures; cartilages are omitted due to limitations of micro-CT scanning. Scale bar equals 3 mm.
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Vertebral column
Vertebral column is diplasiocoelus, typically comprising eight presacral vertebrae (PSV) (Fig. 6C), with the exception of extremely miniaturized species M.nepenthicola, which has PSV I and II fused (Fig. 6D). PSV II–VII procoelous and VIII amphicoelous. Transverse processes of PSV II–IV longer and wider than V–VIII, transverse processes of PSV VI–VIII oriented anterolaterally; orientation of transverse processes to other vertebrae varies (Suppl. material 5: Table S5). Transverse processes of sacrum moderately expanded, with distal end about twice as wide as proximal end. Urostyle shorter than trunk vertebrae, bearing a weak dorsal crest that tapers posteriorly and vanishes at two‐thirds of urostyle length (Fig. 6); its articulation with sacrum is bicondylar.
Axial skeleton composition in the Microhyla–Glyphoglossus assemblage representatives. The vertebral columns are shown in dorsal view for Glyphoglossusmolossus (A), Glyphoglossusyunnanensis (B), Microhylaachatina (C), Microhylanepenthicola (D) and Nanohylaarboricola (E). Numerals (I–VIII) correspond to the numbers of presacral vertebrae (PSV); I+II denotes fusion of the two first PSV. Note: figures display only calcified structures; cartilages are omitted due to limitations of micro-CT scanning. Scale bar equals 3 mm.
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Appendicular skeleton
Pectoral girdle with a firmisternal arrangement. Coracoids, scapulae, and suprascapulae present; first two fully ossified; suprascapula largely chondrified. Coracoids robust with wide proximal end. Omosternum generally absent, except for M.puchra, where a tiny cartilaginous omosternum is present (Suppl. material 5: Table S5). Procoracoids indistinct. Clavicles absent. Cartilaginous sternum large, partially mineralized, fan-shaped or bifurcate (Suppl. material 10: Figure S5D, E); xiphisternum completely cartilaginous.
Hand skeleton including seven largely calcified carpal elements: carpale distale II, carpale distale III–V fused into a single large element, prepollex (consisting of two elements), Element Y, radiale, and ulnare (Fig. 7C–D). Metacarpals long and fully ossified; hand phalangeal formula: 2-2-3-3; all phalanges ossified; distal phalanx of finger III simple, conical-, bobbin- or T-shaped. Foot skeleton with four tarsal elements, including ossified tarsale distale II–III, centrale and a prehallux; prehallux mineralized in all species examined (Suppl. material 10: Figure S5A). Metatarsals fully ossified, long and relatively more massive than metacarpals; foot phalangeal formula: 2-2-3-4-3; all phalanges ossified. Terminal phalanges of toe III T-shaped or simple.
(B) Microhyla II clade
This is a compact clade of nine species belonging to the M.annectens group previously recovered by Gorin et al. (2020), encompassing minute- or small-sized terrestrial or semi-arboreal species with short triangular-shaped body habiti, inhabiting montane forests in Indochina and Sundaland (Fig. 2). The clade Microhyla II is rather uniform in skeletal composition, and examined species share a set of osteological characters that clearly separate this group from the two other clades of the Microhyla–Glyphoglossus assemblage (Suppl. material 5: Table S5).
Skull
Skull longer than wide or almost equal (Figs 4, 5); widest portion posterior, giving head triangular shape (Fig. 5). Frontoparietals longer than broad, narrowing anteriorly, in contact along medial border and fused posteriorly, lacking any dorsal crests; posteriorly fused with exoccipitals. Exoccipitals completely fused with each other (Fig. 5), except M.pulchella, (partial; Suppl. material 5: Table S5). Nasals large, broadly separated, chondrified peripherally, processus paraorbitalis narrow and cultriform, posterior edge concave, anterior edge oblique (Fig. 5). Spenethmoids ossified, completely fused with parasphenoid (in M.pulchella an indistinct suture remains laterally, so the fusion is partial), extending posteroventrally nearly to level of prootics along parasphenoid. Prootics ossified anteromedially, crista parotica entirely cartilagionous. Squamosal ossified, with well-developed long ventral and otic rami and poorly developed zygomatic ramus (Fig. 5O; Suppl. material 5: Table S5). Operculum mineralized. Columella largely mineralized with only pars externa plectra cartilaginous, tympanic annulus completely chondrified (Suppl. material 10: Figure S5G). Premaxilla well-ossified, alary process oriented slightly anteriorly, distal part bending laterally. Maxilla well-ossified; anteriorly in contact with labial portion of premaxilla; edentate; pars facialis moderately high in lateral view. Quadratojugal reduced further than Microhyla I clade, not in anterior contact with maxilla; support of upper jaw taken over by pterygoid. Pterygoid with long anterior ramus (pronounced concavity along ramus that is much more laterally oriented than in Microhyla I clade), broad posterior ramus, and short medial ramus. Vomers small, widely separated, triangular in shape. Neopalatines present as very thin elements. Mentomeckelians ossified, connected to dentaries and to each other through Meckel’s cartilage. Dentary fused with angulosplenial. Parasphenoid smooth; cultriform process of parasphenoid broad, completely fused with sphenethmoid laterally (Fig. 5O), terminating at level of neopalatines with a chondrified notch. Hyoid plate completely cartilaginous, anterolateral processes of hyoid plate present, recurved, posterolateral processes slender, posteromedial processes strongly ossified, elongated, straight, chondrified at distal ends, wider at proximal ends, separated by a chondrified parahyoid.
Vertebral column
Vertebral column diplasiocoelus, including eight presacral vertebrae, with the exception of one of the smallest species of the group, M.arboricola, which has PSV I and II fused (Fig. 6E). PSV II–VII procoelous and VIII amphicoelous. Transverse processes of PSV II–IV longer and wider than in PSV V–VIII; transverse processes of PSV II, VII and VIII oriented anterolaterally, IV and V posterolaterally, III and VI perpendicular to vertebral column axis, with exception of M.marmorata, which has transverse processes of PSV VI oriented anterolaterally. Transverse processes of sacrum notably expanded, with distal end more than twice as wide as proximal. Urostyle shorter than trunk vertebrae, bearing a weak dorsal crest, tapering posteriorly; vanishes completely at 2/3 urostyle length (Fig. 6E).
Appendicular skeleton
Pectoral girdle firmisternal. Coracoids, scapulae, and suprascapulae present; coracoid and scapula fully ossified; suprascapula largely chondrified. Coracoids robust with wide proximal end. Cartilaginous omosternum present (Suppl. material 10: Figure S5F). Procoracoids indistinct. Clavicles absent. Sternum large, cartilaginous, partially mineralized, bifurcate or fan-shaped; xiphisternum completely cartilaginous.
Manus skeleton with seven largely calcified carpal elements, including carpale distale II, carpale distale III–V (fused into a single large element), prepollex (consisting of two separate elements), Element Y, radiale, and ulnare (Fig. 7E). Metacarpals long and fully ossified; phalangeal formula: 2-2-3-3; all phalanges ossified, with exception of M.arboricola, in which metacarpals and phalanges ossified only peripherally (Fig. 7E); distal phalanx of finger III T-shaped. Foot skeleton with four tarsal elements, including ossified tarsale distale II–III, centrale and a prehallux; prehallux cartilaginous in all species examined (Suppl. material 10: Figure S5B). Metatarsals fully ossified, elongated and much more massive than metacarpals; phalangeal formula: 2-2-3-4-3; all phalanges ossified. Terminal phalanges of toe III T-shaped.
Hand skeleton composition in the Microhyla–Glyphoglossus assemblage representatives. The hands are shown in ventral view for Glyphoglossusmolossus (A), Glyphoglossusyunnanensis (B), Microhylaachatina (C), Microhylanepenthicola (D) and Nanohylaarboricola (E). Note: figures display only calcified structures; cartilages are omitted due to limitations of micro-CT scanning. Scale bar equals 1 mm.
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Summary of osteological differences between Glyphoglossus, Microhylas. str. and Nanohyla gen. nov. Diagnostic features of the new genus that are subjectively considered by us to be most reliable are highlighted in bold. Asterisk (*) denotes states observed in G.molossus exclusively. For species-specific data, see Suppl. material 5: Table S5.
Character
Glyphoglossus
Microhylas. str.
Nanohyla gen. nov.
Skull shape
Wider than long
Subequal, longer than wide, or wider than long
Subequal or longer than wide
Frontoparietal–exoccipital junction
Separated
Separated
Fused
Exoccipitals
Separated
Separated
Fused or incompletely fused
Nasal capsules
Ossified
Ossified, partly mineralized, or cartilaginous
Ossified, partly mineralized, or cartilaginous
Neopalatines
Obscured
Present or absent
Present
Maxillary teeth
Present or absent*
Absent
Absent
Vomers
Large
Small
Small
Vomerine teeth
Present or absent*
Absent
Absent
Anterior ramus of pterygoid
Thin and blunt or massive and blunt*
Thin, blunt
Thin, tapered
Sphenethmoid and parasphenoid
Separated
Separated
Fused or incompletely fused
Otic ramus of squamosal
Long
Short
Long
Tympanic annulus
Present
Present or reduced
Present
Columella
Fully ossified
Poorly mineralised
Poorly mineralised
Crista parotica
Fully ossified
Posteriorly ossified
Cartilaginous
Clavicles
Present or absent*
Absent
Absent
Omosternum
Absent
Usually absent
Present
Prehallux
Ossified
Mineralized
Cartilaginous
Terminal phalanges of finger III
Simple
T-shaped, knobbed, or simple
T-shaped
Distance between vomers
Narrow
Wide
Wide
(C) Glyphoglossus clade
In our analysis, three species of Glyphoglossus (of nine recognized) were examined, so the variation of skeletal characters in this genus might be underestimated. All Glyphoglossus species are adapted to fossorial lifestyle, and are easily distinguished from all other members of the group by their large body size, stocky and globular habitus, and enlarged inner metacarpal tubercle used for burrowing. Species of Glyphoglossus inhabit lowland areas of southern mainland China, Indochina, and Sundaland (Fig. 2). A broad range of morphological variation has been documented: G.molossus is notably different from G.yunnanensis and G.guttulatus (until recently, both were classified as members of the genus Calluella Stoliczka, 1872, now considered a junior synonym of Glyphoglossus based on its phylogenetic placement; Peloso et al. 2016). Owing to the morphological uniqueness of G.molossus, morphological features of this species are marked with an asterisk (*).
Skul
Skull notably wider than long (Fig. 4). Skull widest at mid-length, giving head a widened, rounded shape. Frontoparietals longer than broad, narrowing anteriorly, connecting medially with a suture along whole length, or anteriorly, separated or fused* (Fig. 5A) medially, lacking dorsal crests, separated or fused* with exoccipitals (separate) posteriorly. Nasals large, separated, chondrified peripherally; processus paraorbitalis well-developed, pointed laterally or anteriorly* (Fig. 5). Spenethmoid separate, well ossified, restricted to anterior third of brain case or nearly closing lateral wall of brain case* (Fig. 5C). Prootics ossified anteromedially or completely*, crista parotica mineralized medially or completely*. Squamosal ossified, with well-developed ventral ramus and less developed, but distinct otic and zygomatic rami. Operculum cartilaginous or ossified*. Columella largely ossified, with only pars externa plectra cartilaginous; tympanic annulus completely chondrified. Premaxilla well-ossified, alary process oriented slightly posteriorly, distal portion straight or bending laterally*. Maxilla well-ossified, anteriorly contacting labial portion of premaxilla; teeth present or absent*; pars facialis moderately to notably high, and oriented towards processus paraorbitalis of nasal*. Quadratojugal robust, with rounded cartilaginous articulation with angulosplenial, anteriorly articulating with or fused* to maxilla. Pterygoid massive, with a long anterior ramus, broad posterior ramus, and short medial ramus. Vomers large, shape either complex or U-shaped*, defining lower floor of nasal capsule. Neopalatines obscured by postchoanal vomerine processes, fused or replaced completely*. Nasal capsules mineralized posteriorly or obscured by postchoanal vomerine processes*. Mentomeckelians ossified, connected to dentaries, and to each other through Meckel’s cartilage. In G.molossus, ventral portion of mentomeckelian cartilage protruding and greatly mineralized, forming a unique beard-like structure, shaping the characteristically flattened snout profile* (Fig. 2). Dentary fused with angulosplenial. Parasphenoid smooth, its cultriform process broad, tapering anteriorly or not*, terminating at level of sphenethmoid or nasal capsules*, with a chondrified notch. Hyoid plate completely cartilaginous, its anterolateral processes well-developed, recurved, posterolateral processes slender, posteromedial processes strongly ossified, elongated, straight, chondrified at distal ends, wider at proximal ends, separated by a chondrified parahyoid. Each posteromedial process bears two bony flanges; one oriented laterally, another medially.
Vertebral column
Vertebral column diplasiocoelus, with eight presacral vertebrae. PSV II–VII procoelous, PSV VIII amphicoelous. PSV I very unusual in shape in G.molossus, with highly extended condylar arms. Transverse processes of PSV II–IV longer and wider than V–VIII, transverse processes of PSV II, VII and VIII oriented anterolaterally, IV and V posterolaterally, III and VI at right angle to vertebral column axis. In G.molossus transverse processes of PSV greatly shortened, II and VI–VIII oriented anterolaterally, IV oriented posterolaterally, III and V at the right angle to the body axis* (Fig. 6A). Sacral transverse processes moderately expanded, with the distal end about twice as wide as the proximal end. The urostyle notably shorter than the trunk vertebrae (Fig. 6), bearing a dorsal crest that tapers posteriorly and vanishes at about one third of the urostyle length (Fig. 6B), or continues almost to the end of the urostyle* (Fig. 6A).
Appendicular skeleton
Pectoral girdle firmisternal. Coracoids, scapulae, and suprascapulae present; first two fully ossified; suprascapulae largely chondrified. Coracoids robust, with proximal end, or both ends widened*. Omosternum absent. Procoracoids present or absent*. Clavicles present or absent*. Cartilaginous sternum large, partially mineralized, fan-shaped; xiphisternum completely cartilaginous.
Hand skeleton with six largely calcified carpal elements: carpale distale II, carpale distale III–V fused into a single large element, prepollex (consisting of two elements), element Y, radiale and ulnare (Fig. 7A–B). Metacarpals long and fully ossified; phalangeal formula: 2-2-3-3; all phalanges ossified, notably shortened in G.molossus*; distal phalanx of finger III simple. Foot with four tarsal elements, including ossified tarsale distale II–III, centrale, and prehallux; prehallux enlarged and ossified (Suppl. material 10: Figure S5C). Metatarsals fully ossified, long, more massive than metacarpals; phalangeal formula: 2-2-3-4-3; all phalanges ossified. Terminal phalanx of toe III simple, conical.
Body size and sexual dimorphism evolution
Clades I and II of Microhyla are inferred to have independently reduced in body size from a moderately small common ancestor (males estimated at 25.3 mm, 95% CI 18.8–34.2; Fig. 8). Within Microhyla I, two clades arose from miniaturized common ancestors, the Microhylasuperciliaris species group (common ancestor estimated at 17.7 mm), and the M.achatina species group (common ancestor estimated at 19.6 mm; a second clade, composed of Microhyla sp. 3 and M.kodial, likely independently reduced in size with a common ancestor of 18.3 mm). A few lineages have also reduced in body size below 20 mm independently (Fig. 8), giving a total of eight transitions to SVL < 20 mm. The common ancestor of all Microhyla II species was apparently miniaturized (male SVL estimated at 18.1 mm), and most lineages reduced further. Two lineages, M.annamensis + M.marmorata and M.pulchella, have increased in body size independently and repeatedly from miniaturized ancestors, to their modern body sizes. In Microhyla I, the M.berdmorei species group substantially increased in body size. Among Glyphoglossus, G.molossus is an extreme outlier in body size, and is substantially larger than other equivalent-level clades. Across the entire assemblage, male SVL exhibits substantial phylogenetic signal (Pagel’s λ = 1.00).
Continuous ancestral state reconstruction of male body size (left) and sexual size dimorphism (right) in the Microhyla–Glyphoglossus assemblage. Species names in purple have at least one sex with maximum SVL ≤ 20 mm, in fuchsia at least one sex with maximum SVL ≤ 16 mm. Circles at nodes are based on inferred ancestral male SVL.
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Sexual size dimorphism exhibits no phylogenetic signal (Pagel’s λ = 7.2 × 10-5), changing sporadically across the tree, and is weakly positively correlated with log(male SVL) (PGLS, F1,51 = 5.478, adjusted R2 = 0.07928, P = 0.02321; Fig. 9B). Most species of Microhyla I and II exhibit female-biased size dimorphism (above the y = x line in Fig. 9A), and among these, species with the smallest males exhibit the greatest degree of size dimorphism (Fig. 9B). Only six species are male-biased (Microhyla sp. 1, M.mantheyi, M.mukhlesuri, M.superciliaris, M.mihintalei, and G.molossus), including both the largest (G.molossus) and the smallest (Microhyla sp. 1) species in our dataset.
Relationships between body size among sexes (A), and between male body size and sexual size dimorphism (B) in Microhylas. str., Nanohyla gen. nov., and Glyphoglossus. The line in (A) represents x=y.
https://binary.pensoft.net/fig/496423Discussion
A fully resolved taxonomic framework should approximate the phylogenetic relationships of its members, allowing the user to roughly infer basic information from the framework itself (Wake 2013). This information includes monophyly of the recognized taxonomic groupings, and their differences in sets of biologically significant traits. A taxonomic framework that allows such information to be accurately inferred maximizes its utility. Additionally, the taxonomic framework should, ideally, be optimized for stability, reducing the need for additional taxonomic changes in future (Vences et al. 2013). All recent phylogenetic studies of the subfamily Microhylinae agree that (i) Glyphoglossus and Microhylas. lat. are closely related, and (ii) Microhylas. lat. consists of two deeply-divergent lineages (Microhyla I and II of Gorin et al. [2020]). The relationship between these two Microhyla clades and Glyphoglossus evidently cannot be resolved with mitochondrial DNA alone (e.g., Matsui et al. 2011; Poyarkov et al. 2018b, 2019; Nguyen et al. 2019; Li et al. 2019; Gorin et al. 2020), likely due to a combination of the considerable age of these splits (>40 Ma), resulting in saturation and loss of phylogenetic signal, and the moderately rapid succession in which they apparently occurred. Nuclear data, especially multilocus datasets, do, however, support the monophyly of Microhylas. lat. (Peloso et al. 2016; Tu et al. 2018; Garg and Biju 2019; Gorin et al. 2020; and the present paper). However, as will become evident in the following, we find there to be substantial evidence supporting the treatment of the two major clades within Microhylas. lat. as separate genera.
Although present evidence indicates that we can be moderately confident in the respective monophyly of Microhylas. lat. and Glyphoglossus, it is also worth noting that the two lineages within Microhylas. lat. are very old. The Microhyla–Glyphoglossus assemblage radiated within a narrow period in the middle Eocene, with the origin of Glyphoglossus dating to 44.1 Ma (38.5–49.6), while the basal split within Microhylas. lat. is estimated at 43.9 Ma (37.8–48.2) (Suppl. material 4: Table S4, Suppl. material 8: Figure S3). These two estimates are very close and their 95% credibility intervals overlap, suggesting near-simultaneous origin of Glyphoglossus, Microhyla I, and Microhyla II. These estimates are notably older than the ages of all other microhyline genera (except Chaperina), which may have diverged in the late Eocene (the split between Micryletta and Mysticellus [40.9 Ma, 33.3–47.7]) or Oligocene (the split between Kaloula and Uperodon 27.4 Ma [19.4–34.9], and the split between Phrynella and Metaphrynella is estimated at 23.0 Ma [16.2–29.1]; Suppl. material 4: Table S4, Suppl. material 8: Figure S3). Similar results were also reported by Garg and Biju (2019), who provided even older estimates for all microhyline genera. Thus, the two clades within Microhylas. lat. are of equal or greater age than other genera in this subfamily. While age has not historically been taken into account in most higher taxonomy, it is nonetheless desirable for taxa of equal rank to be of generally comparable age (Hennig 1966; Vences et al. 2013).
In addition to their substantial age, we have identified a number of important osteological and external morphological differences that distinguish the three clades within this assemblage, including the two clades within Microhylas. lat. These include body size and shape, number and shape of metatarsal tubercles, adaptation to burrowing lifestyle, extent of toe webbing, relative size of the first finger (FI) (Fig. 10), and the presence of an external tympanum (Fig. 11). The absence of an externally visible tympanum traditionally was regarded as one of the key diagnostic characters of the genus Microhyla (Boulenger, 1882; Parker 1934; Garg et al. 2019). In all species of Microhyla I, the tympanum is hidden under the skin of the supratympanic fold. However, a closer examination of all species of Microhyla II demonstrates that six (of nine) taxa actually have an external tympanum that is discernable in breeding males (Fig. 11). The presence of an externally visible tympanum in the majority of the Microhyla II species suggests it may be an important character for diagnosing this clade from Microhyla I (Suppl. material 5: Table S5).
Palmar views of hands (above) and thenar views of feet (below) of the representative Microhylas. str. and Nanohyla gen. nov. species: N.annamensis (A, B), N.arboricola (C, D), M.minuta (E, F), and M.tetrix (G, H). Arrow indicates outer metatarsal tubercle. Not to scale. Line drawings by Valentina D. Kretova.
Members of the new genus Nanohyla gen. nov. in life (males): N.annectens from Genting Highlands, Pahang, Malaysia (A), N.annamensis from Bidoup – Nui Ba N.P., Lam Dong, Vietnam (B), N.arboricola from Chu Yang Sin N.P., Dak Lak, Vietnam (C), N.hongiaoensis from Bidoup – Nui Ba N.P., Lam Dong, Vietnam (D), N.marmorata from Kon Chu Rang N.R., Gia Lai, Vietnam (E), N.nanapollexa from Kon Plong, Kon Tum, Vietnam (F), N.perparva from Gunung Mulu, Sarawak, Malaysia (G), N.petrigena from Gunung Mulu, Sarawak, Malaysia (H), and N.pulchella from Bidoup – Nui Ba N.P., Lam Dong, Vietnam (I). Insets show tympanic area of the each species; white arrow points at the tympanic rim of the external tympanum. Photos by Nikolay A. Poyarkov (A–С, D–F, I), Vu Dang Hoang Nguyen (D), and Indraneil Das (G, H).
https://binary.pensoft.net/fig/496425
Furthermore, there are pronounced differences in the patterns of geographical distribution among the three clades of the Microhyla–Glyphoglossus assemblage (Fig. 1) which, along with their ecological differences, suggest that they may warrant recognition as separate genera of Microhylinae. The available hypothesis of the biogeographic history of this assemblage (Gorin et al. 2020) demonstrated that the group originated in Southeast Asia. The smaller members of Microhyla II clade are closely associated with perhumid montane forests, and their distribution is limited by mountain ridges among Borneo, the Thai-Malay Peninsula and Indochina (Fig. 1). At the same time, large-sized burrowing species of Glyphoglossus can aestivate during the dry season, and have become more widely distributed across Southeast Asia and seasonally dry plains of Central Indochina and Myanmar (Fig. 1; Gorin et al. 2020). Microhyla I is the most diverse clade in terms of morphological and ecological adaptations, and species of this group have colonized almost the entire Asian Realm, including southern and eastern China (Fig. 1).
The cumulative evidence suggests to us that continuing to recognize the superficially similar Microhyla I and II clades as members of a single genus would conceal information on the ancient divergence between these lineages, as well as the differences between them in a number of biologically relevant organismal traits. Put another way, recognizing the two clades as separate genera would enhance the diagnosability of the respective genera, make them more comparable units to other genera, and fully stabilize the taxonomy of the Microhyla–Glyphoglossus assemblage (if coalescent phylogenomic reconstructions were to reveal the clades to be paraphyletic with respect to Glyphoglossus, no taxonomic changes would be necessary). Splitting them would therefore be in accordance with all three of the Priority Taxon Naming Criteria (TNCs) of Vences et al. (2013): Monophyly, Clade Stability, and Diagnosability, as well as the secondary TNCs Time Banding and Biogeography. We also contend that this solution is superior to the obvious alternatives, which are (i) sinking all three clades into a single genus, or (ii) recognizing the two clades within Microhylas. lat. as subgenera. The former would maximize monophyly and clade stability, but would seriously compromise the diagnosability of the genus, whereas the latter would continue to satisfy the three priority TNCs but would not optimize under the Time Banding TNC. In the following, we therefore divide Microhylas. lat. (hitherto containing 52 species), into two genera consisting of 43 (Microhyla I) and nine (Microhyla II) species each. As there are no available names for Microhyla II, we formally describe it as a new genus, and provide revised taxonomic accounts of Microhylas. lat. and Glyphoglossus.
Microhyla (partim)–Boulenger 1900; Smith 1923; Inger and Frogner 1979; Inger 1989; Bain and Nguyen 2004; Poyarkov et al. 2014; Hoang et al. 2020.
Microhyla (Microhyla) (partim)–Dubois 1987 (as a part of the subgenus Microhyla).
Type species.
Microhylaannectens Boulenger, 1900.
Etymology.
The genus name is derived from the Greek νᾶνος (nanos), meaning “dwarf”, “pygmy”, and the mythological figure, Hylas (Ancient Greek: Ὕλας), which is probably derived from the Ancient Greek verb “ὕλαω” meaning “to bark” (Bourret 1942). In classical mythology, Hylas, son of King Theiodamas, was a youth who served as Heracles’ companion, lover, and servant. Heracles took Hylas with him on the Argonauts’ expedition, during which Hylas was kidnapped by nymphs of the spring in Pegae, Mysia, and turned into an echo. Heracles left the ship and was searching for Hylas for a great length of time, calling his name: “His adjunxit Hylan nautae quo fonte relictum / Clamassent ut littus Hyla! Hyla! omne sonaret” (“The mariners cried on Hylas till the shore / Then Re-echoed Hylas! Hylas! soothed...”; Virgil 1916, Ecl. 6, 43). The genus name refers to the small body size (< 25 mm) of all known Nanohyla species, while maintaining resemblance to its sister genus Microhyla, from which it is separated herein. The new genus name is feminine in gender.
Suggested common name.
Pygmy Narrow-mouthed Frogs.
Taxonomic content.
Nine species, including: Nanohylaannamensis comb. nov. (Smith, 1923); Nanohylaannectens comb. nov. (Boulenger, 1900); Nanohylaarboricola comb. nov. (Poyarkov, Vassilieva, Orlov, Galoyan, Tran, Le, Kretova & Geissler, 2014); Nanohylahongiaoensis comb. nov. (Hoang, Nguyen, Luong, Nguyen, Orlov, Chen, Wang & Jiang, 2020); Nanohylamarmorata comb. nov. (Bain & Nguyen, 2004); Nanohylananapollexa comb. nov. (Bain & Nguyen, 2004); Nanohylapetrigena comb. nov. (Inger & Frogner, 1979); Nanohylaperparva comb. nov. (Inger & Frogner, 1979); and Nanohylapulchella comb. nov. (Poyarkov, Vassilieva, Orlov, Galoyan, Tran, Le, Kretova & Geissler, 2014). Photos of Nanohyla gen. nov. members are presented in Fig. 11.
Diagnosis.
The new genus is assigned to the subfamily Microhylinae on the basis of phylogenetic affinities and the following combination of morphological character states: vomers small, confined to the anterior and medial margins of choanae; clavicles and, in most cases, procoracoids absent, maxillary arcade edentate (Parker 1934). Nanohyla gen. nov. differs from other Microhylinae genera by the following combination of osteological character states: (1) frontoparietals fused with exoccipitals; (2) exoccipitals fused with each other (incomplete fusion in N.pulchella); (3) neopalatines present; (4) sphenethmoids completely fused with parasphenoid (incomplete fusion in N.pulchella); (5) crista parotica entirely cartilaginous; (6) otic ramus of squamosal well-developed; (7) tympanic annulus well-developed; (8) transverse processes of presacral vertebrae with the following orientation: IV and V posterolaterally, II, VII and VIII anterolaterally, III and VI at right angle to body axis; (9) clavicles absent; (10) omosternum present, cartilaginous; (11) prehallux cartilaginous; (12) terminal phalanges of longest fingers and toes T-shaped. The combination of diagnostic external morphological characters includes: (13) small to extremely small frogs (adult SVL 11.8–25.8 mm); (14) snout rounded or pointed in profile; (15) supratympanic fold present; (16) ridge on posterior sides of choanae absent; (17) first finger (FI) length less than ½ FII or reduced to a nub; (18) finger discs present, at least on FII–FIV; (19) dorsal median longitudinal grooves on finger discs generally present (with the exception of N.perparva); (20) toes dorsolaterally flattened, prominent discs present; (21) dorsal median longitudinal grooves on toe discs present; (22) metatarsal tubercle single (inner metatarsal tubercle present, outer absent); (23) dorsomedial line absent; (24) superciliary tubercles absent; (25) tibiotarsal articulation of adpressed hindlimb reaching well beyond snout; (26) toe webbing well-developed (at least one-half webbed); (27) skin on dorsum feebly granular to tubercular; (28) tympanum externally distinct at least in males (N.annamensis, N.annectens, N.arboricola, N.marmorata, N.nanapollexa, N.pulchella) or barely distinct (N.hongiaoensis, N.perparva, N.petrigena); (29) terrestrial or scansorial semi-arboreal microhabitat preference.
Phylogenetic definition.
The genus Nanohyla gen. nov. includes all species sharing a more recent common ancestor with Nanohylaannectens than with Microhylaachatina and Glyphoglossusmolossus.
Distribution.
The distribution area of Nanohyla gen. nov. covers montane forests of the Annamite (Truong Son) Mountains in Vietnam, eastern Laos, and north-eastern Cambodia, the Titiwangsa Mountain Range in the southernmost Thailand and peninsular Malaysia, mountains of Borneo (including Sabah and Sarawak of Malaysia, Brunei, and Kalimantan of Indonesia) and the Sulu Archipelago of the Philippines (see Fig. 1). The occurrence of Nanohyla gen. nov. in Cardamom Mountains in eastern Thailand (the record of “M.annamensis” from Khao Sebab by Taylor [1962], see Fig. 1) is questionable (see Poyarkov et al. 2014, 2020a).
Morphological comparison.
The new genus Nanohyla gen. nov. differs from its sister genus Microhyla Tschudi, 1838 s. str. by the well-developed (vs poorly-developed) otic ramus of the squamosal, frontoparietals and exoccipitals fused (vs separated or slightly fused), exoccipitals fused with each other (vs always separated), omosternum present (vs usually absent), sphenethmoid and parasphenoid fused completely or partially (vs separated), cartilaginous crista parotica (vs mineralized posteriorly), cartilaginous prehallux (vs mineralized), tympanum externally visible or barely visible (vs concealed beneath skin), inner metatarsal tubercle well-developed, outer generally absent (vs two metatarsal tubercles well-developed), and in having digits dorso ventrally flattened, FI often reduced to a nub or shortened (vs variably longer). The new genus differs from the closely related genus Glyphoglossus Günther, 1869 by its smaller adult size with SVL < 25mm (vs SVL > 25mm), skull longer than wide or almost equal (vs wider than long), alary process of premaxilla oriented slightly anteriorly (vs posteriorly), neopalatines present (vs obscured by vomers), vomers small, indistinct (vs large, well-developed), omosternum present (vs absent), terminal phalanges T-shaped (vs simple), tibio-tarsal articulation reaching well beyond snout (vs to the anterior border of the eye, or less), by body habitus short, triangular-shaped (vs stout, balloon-shaped), and by inner metatarsal tubercle not enlarged (vs enlarged, shovel-shaped). Nanohyla gen. nov. differs from Kaloula Gray, 1831 by its much smaller adult body size SVL < 25 mm (vs SVL > 38 mm), procoracoids absent (vs present), postchoanal portion of vomer absent (vs present), neopalatines present (vs obscured), prehallux formed by two elements (vs one), tibio-tarsal articulation reaching well beyond snout (vs to shoulder), absence (vs presence) of ridge on posterior margin of choanae, inner metatarsal tubercle not enlarged (vs enlarged and spatulate), and by body habitus short, triangular-shaped (vs robust). The new genus can be distinguished from Uperodon Duméril & Bibron, 1841 by its smaller adult size, SVL < 25 mm (vs SVL > 34 mm), postchoanal portion of vomer absent (vs present), neopalatines present (vs obscured), tibio-tarsal articulation reaching well beyond snout (vs posterior border of eye, or less), absence (vs presence) of ridge on posterior margins of choanae, inner metatarsal tubercle not enlarged (vs enlarged or spatulate), and by body habitus short, triangular-shaped (vs robust and globular). Nanohyla gen. nov. differs from Phrynella Boulenger, 1887 by its smaller adult size, SVL < 25 mm (vs SVL > 30 mm), medial process of the prechoanal part of vomer absent (vs present), neopalatines present (vs absent), procoracoids absent (vs present), vertebral column diplasiocoelus (vs procoelus), metatarsal tubercules separate (vs united), by tibio-tarsal articulation reaching well beyond snout (vs to tympanic region), by body habitus short, triangular-shaped (vs robust and flattened), and by generally dull brownish coloration of inguinal and dorsal surfaces (vs greenish coloration of dorsum and bright-red coloration of inguinal area, and ventral surfaces of limbs). The new genus further differs from Metaphrynella Parker, 1934 by its smaller adult size, SVL < 25 mm (vs SVL > 25 mm), skull longer than wide or almost equal (vs wider than long), neopalatines present (vs absent), omosternum present (vs absent), vertebral column diplasiocoelus (vs procoelus), tibio-tarsal articulation reaching well beyond snout (vs to tympanic region), absence (vs presence) of a ridge on posterior margins of choanae, metatarsal tubercules separate (vs united and enlarged), and by finger webbing absent (vs present). The new genus differs from Mysticellus Garg & Biju, 2019 by its short triangular-shaped body habitus (vs slender), supratympanic fold present (vs absent), finger and toe tips enlarged with prominent discs (vs slightly enlarged), toe webbing well-developed (vs rudimentary), supernumerary carpal tubercles absent (vs prominent subarticular tubercles alternating with additional smaller tubercles), and the two prominent blackish-brown ‘false-eye’ inguinal spots absent (vs present). Nanohyla gen. nov. differs from Micryletta Dubois, 1987 by its snout longer than eye diameter, and having eye less (vs more) prominent in lateral and dorsal aspects, finger and toe tips enlarged with prominent discs (vs slightly enlarged), toe webbing well-developed (vs rudimentary or absent), supernumerary carpal tubercles absent (vs present), omosternum present (vs absent), neopalatines present (vs absent), tibio-tarsal articulation reaching well beyond snout (vs to anterior border eye, or less), supratympanic fold present (vs absent), and body habitus short, triangular-shaped (vs slender). Finally, the new genus is distinguished from Chaperina Mocquard, 1892 by clavicles and procoracoids absent (vs present), postchoanal portion of vomer absent (vs present), omosternum present (vs absent), terminal phalanges T-shaped (vs simple), tibiotarsal articulation reaching well beyond snout (vs anterior border of eye), belly dull-colored (vs bright saffron-yellow belly with dark pattern), and by absence of spine-like projections on limbs (vs a long, narrow dermal spine projecting from calcaneus).
Larval morphology.
Description of the larval stages of the Nanohyla gen. nov. members are sparse and often not detailed. Poyarkov et al. (2014) provided descriptions, photos and illustrations of tadpole morphology for N.annamensis, N.arboricola and N.pulchella. Vassilieva et al. (2017) provided a detailed description of development, larval morphology and anatomy for N.arboricola. Le et al. (2016) provided a brief description of tadpole morphology of N.marmorata. Leong (2004) provided a short description and photographs of larval and metamorph morphology for N.annectens. Brief descriptions and figures depicting larvae of N.petrigena and N.perparva are found in the original description of these species by Inger and Frogner (1979), as well as in Inger and Steubing (2005) and Haas et al. (2020). Larval stages of N.hongiaoensis and N.nanapollexa remain unknown.
As with almost all larvae in Microhylidae, labial teeth and mandibles are absent from the oral discs of Nanohyla tadpoles. Most species of Nanohyla have larval morphology resembling that of many pond-breeding Microhyla species (Poyarkov et al. 2014) with rather short-tailed transparent or semi-transparent Orton’s type II tadpoles (Orton 1953), that are mid-water column (neustonic) feeders with comparatively unexpanded lower labium and anteriorly directed terminal mouths, lateral orientation of eyes, spiraculum located in a medial position on the venter, spiracular flap with crenulate margins, and tail lacking terminal filament (Altig and Johnston 1989; Donnelly et al. 1990; Leong 2004). In contrast, many species of Microhylas. str. are surface suspension feeders, and demonstrate greatly expanded lower labium and dorso-terminal mouth orientation; they may have terminal filament on tail and smooth margins of spiracular flap (e.g., Leong 2004; Hendrix et al. 2008; Poyarkov et al. 2014).
A peculiar exception is the case of N.arboricola, which is an obligate phytotelm-breeding species that reproduces in water-filled tree hollows (Vassilieva et al. 2017). The oophagous tadpoles of this species differ from larvae of pond-dwelling Microhyla and Nanohyla species in many aspects, including external morphology (extremely long tails, dorsolateral position of the eyes, dark pigmentation), morphology of digestive tract (large, extensible stomach with comparatively short intestine), and characteristic oral morphology (Vassilieva et al. 2017). Nanohylananapollexa was suggested as phytotelm-breeder as a single specimen of this species was recorded in a water-filled tree hollow (Gorin et al. 2020), although the details of reproductive biology and tadpole morphology of this species are still unknown.
Taxonomic comment.
Microhylapulverata Bain & Nguyen, 2004 was considered a junior synonym of N.marmorata based on the phylogenetic results of Gorin et al. (2020); the same study also reported on three putative candidate species within N.arboricola, N.perparva, and N.petrigena, indicating that our knowledge on diversity of Nanohyla is still incomplete.
Certain variation in diagnostically important characters of Nanohyla gen. nov. requires further comments. Bain and Nguyen (2004) reported on significant variation in size and shape of the outer metatarsal tubercle in N.marmorata which was reported to vary from almost indistinct to “conical.” We have examined a large series of N.marmorata (see Poyarkov et al. 2014; Nguyen et al. 2019) and found that in this species the outer metatarsal tubercle usually is not discernable or is indistinct; we assume that this discrepancy might be explained with the differences in preservation of specimens examined by us and by Bain and Nguyen (2004). Hoang et al. (2020) reported two metatarsal tubercles in their diagnosis of N.hongiaoensis, however in the holotype description they refer to the outer metatarsal tubercle as “indistinct;” it is also not discernable in their photo of holotype’s foot (Hoang et al. 2020:fig. 3F). In all the remaining species of Nanohyla gen. nov. it is absent, and we therefore consider this state to be diagnostic for the genus (in comparison to Microhylas. str., which has two metatarsal tubercles in all species but M.maculifera, see comment below). It is not clear why Bain and Nguyen (2004), or Poyarkov et al. (2014; and other preceding studies) did not recognize the presence of externally visible tympanum in most of species of the genus (Fig. 11). In species of Nanohyla gen. nov., smaller tubercles and other dermal structures of the skin become flattened and less distinct after fixation and preservation; this has also been reported in other anurans (Poyarkov et al. 2015, 2017, 2019; Nguyen et al. 2018, 2019, 2020). It is likely that the presence of the tympanum was artifactually concealed from Bain and Nguyen (2004), since their description was based exclusively on museum specimens. In some species of Nanohyla gen. nov., we were not able to detect an externally visible tympanum (N.hongiaoensis, N.perparva, N.petrigena). It is not clear whether this reflects an actual character state in these species, or if this apparent state relates to the small sample size of specimens and photographs available to us. Further studies are needed to clarify variation of the external tympanum in Nanohyla gen. nov.
Microhylas. str. differs from all other Microhylinae genera by the following combination of osteological characters: (1) frontoparietals generally separated from exoccipitals (partially fused in M.mukhlesuri, M.picta and Microhyla sp. 2); (2) exoccipitals separate; (3) neopalatines present (in M.berdmorei, M.butleri, M.minuta, M.orientalis, M.pineticola, M.superciliaris and M.tetrix) or absent (in M.achatina, M.heymonsi, M.fissipes, M.malang, M.mukhlesuri, M.nepenthicola, M.nilphamariensis, M.okinavensis, M.picta, M.pulchra and Microhyla sp. 2); (4) sphenethmoids not fused to parasphenoid; (5) crista parotica ossified posteriorly; (6) otic ramus of squamosal poorly developed; (7) tympanic annulus well-developed (reduced in M.heymonsi, M.nepenthicola, M.nilphamariensis, M.orientalis, M.pineticola, M.superciliaris and M.tetrix); (8) orientation of transverse processes of presacral vertebrae VI–VIII anterolateral, other vertebrae with inconsistent orientation; (9) clavicles absent; (10) omosternum absent (cartilaginous omosternum present only in M.pulchra); (11) prehallux cartilaginous; (12) terminal phalanges of the longest fingers T-shaped (in M.achatina, M.berdmorei, M.butleri, M.fissipes, M.heymonsi, M.malang, M.minuta, M.nepenthicola, M.nilphamariensis and M.pineticola), knobbed (in M.minuta, M.mukhlesuri, M.nilphamariensis, M.superciliaris and M.tetrix), or simple (in M.okinavensis, M.orientalis, M.picta and M.pulchra), terminal phalanges of the longest toe T-shaped (in M.achatina, M.berdmorei, M.butleri, M.heymonsi, M.malang, M.nepenthicola and M.pineticola), knobbed (in M.minuta, M.mukhlesuri, M.nepenthicola, M.superciliaris and M.tetrix), or simple (in M.fissipes, M.okinavensis, M.orientalis, M.picta, M.pulchra and Microhyla sp. 2). The combination of diagnostic external morphological characters includes: (13) body size medium to extremely miniaturized (adult SVL 12.8–45.8 mm); (14) snout rounded or pointed in profile; (15) supratympanic fold present; (16) ridge on posterior margins of choanae absent; (17) FI length greater than ½ FII; (18) discs present on every finger, only FII–FIV, or absent; (19) dorsomedial grooves on fingers present or absent; (20) toe discs present or absent; (21) dorsomedial grooves on toes present or absent; (22) two metatarsal tubercles (except M.maculifera with a single metatarsal tubercle); (23) dorsomedial line present or absent; (24) superciliary tubercles present (M.palmipes and M.superciliaris) or absent (all remaining species); (25) tibiotarsal articulation reaching well beyond snout (in M.berdmorei, M.darevskii, M.mantheyi and M.tetrix) or less; (26) toe webbing from basal to developed to discs; (27) skin on dorsum from smooth to tubercular; (28) tympanum externally indistinct; (29) terrestrial or subfossorial microhabitat preference.
Phylogenetic definition.
The genus Microhylas. str. includes all species that share a more recent common ancestor with Microhylaachatina than with Nanohylaannectens and Glyphoglossusmolossus.
Distribution.
Frogs of the genus Microhyla are widely distributed across the East (southern China, including Taiwan and Hainan islands, and Ryukyu Archipelago of Japan), Southeast (Myanmar and Indochina, Malayan Peninsula, Sumatra, Java, Bali, and Borneo), and South Asia (Bangladesh, Nepal, Indian subcontinent to north-eastern Pakistan in the west and Sri Lanka in the south) (Fig. 1).
Taxonomic comment.
In the last phylogenetic revision of Microhyla, Gorin et al. (2020) included all species of the genus in their analysis, except M.darevskii, M.fusca Andersson, 1942, and M.maculifera. Microhyladarevskii was described from five formalin-fixed specimens and morphologically appears to be very close to the members of M.berdmorei species complex (Poyarkov et al. 2014). Although the phylogenetic position of M.darevskii is not known, this species can be confidently assigned to the genus Microhylas. str. based on morphological data. Microhylafusca was described from a single specimen collected from southern Vietnam (Andersson 1942), and was recently demonstrated to be a junior synonym of M.butleri (Poyarkov et al. 2020a).
Microhylamaculifera remains the most enigmatic species of the group due to the lack of molecular data and uncertainties regarding morphological characters. This species was described from only two specimens (Inger 1989), and no additional specimens have been reported since that time, despite numerous field survey efforts. This small-sized species is unique among its congeners in having comparatively short hindlimbs, large and wide head, less triangular than in other Microhyla, comparatively stout body habitus (Fig. 12), and a single metatarsal tubercle (vs two). Microhylamaculifera is different from the members of the genus Nanohyla gen. nov. by having FI longer than ½ of FII (vs FI shorter than ½ of FII or reduced to a nub), lack of discs on fingers and rudimentary discs on toes (vs digital discs well-developed), absence (vs presence) of dorsal median grooves on tips of fingers and toes, having comparatively short hindlimbs with tibiotarsal articulation reaching to snout (vs to well beyond snout), and toe webbing being basal (vs well-developed; Inger 1989). Due to the lack of molecular data, the phylogenetic position and generic placement of “Microhyla” maculifera remains uncertain; we tentatively retain this species Microhylas. str. pending data or future phylogenetic studies, which might suggest another arrangement.
Holotype of Microhylamaculifera Inger, 1989 (FMNH 231271, adult male) in dorsal (A) and ventral (B) aspects. Scale bar denotes 5 mm. Field Museum of Natural History. FMNH 231271. Created by Field Museum of Natural History, Amphibian and Reptile Collection and licensed under CC-BY-SA 4.0.
Glyphoglossus Günther, 1869 differs from other Microhylinae genera by the combination of the following osteological characters: (1) frontoparietals separated from exoccipitals (fused to them in G.molossus); (2) exoccipitals separated from each other; (3) neopalatines obscured by a postchoanal portion of vomers; (4) sphenethmoids separated from parasphenoid; (5) crista parotica ossified; (6) otic ramus of squamosal well-developed; (7) tympanic annulus well-developed; (8) orientation of transverse processes of presacral vertebrae as follows: IV and V posterolateral, II, VII and VIII anterolateral, III and VI at right angle to body axis (in G.molossus IV posterolateral, II, VI-VIII anterolateral, III and V at right angle to body axis); (9) clavicles present (absent in G.molossus); (10) omosternum absent; (11) prehallux ossified; (12) terminal phalanges of the longest finger and toe simple. The combination of diagnostic external morphological characters includes: (13) large to medium-sized frogs (adult SVL 30.9–94.9 mm); (14) snout rounded or bluntly flattened; (15) supratympanic fold present; (16) ridge on posterior margins of choanae poorly developed or absent; (17) first finger (FI) length greater than ½ FII; (18) discs on digits absent; (19) two metatarsal tubercles; (20) dorsomedial line absent; (21) superciliary tubercles absent; (22) tibiotarsal articulation of the adpressed hindlimb reaching eye or shorter; (23) toe webbing moderately developed (at least one-third webbed, in G.molossus three-quarters webbed); (24) skin on dorsum from feebly granular to tubercular; (25) external tympanum invisible; (26) fossorial microhabitat preference.
Phylogenetic definition.
The genus Glyphoglossus includes all species sharing a more recent common ancestor with Glyphoglossusmolossus than with Microhylaachatina and Nanohylaannectens.
Distribution.
From south-western China across Indochina to Myanmar, Thai-Malay Peninsula, islands of Sumatra and Borneo (Fig. 1).
Taxonomic comment.
Until recently Glyphoglossus was considered to be a monotypic genus, until it was synonymized with Calluella based on phylogenetic data of Peloso et al. (2016). However, available phylogenetic studies (Tu et al. 2018; Garg and Biju 2019; Gorin et al. 2020) have not all included comprehensive sampling of Sundaland species (e.g., C.volzi, C.smithi, C.flavus, and C.brooksi). In our opinion, the variable taxonomic sampling included in previous analyses (Matsui et al. 2011; Peloso et al. 2016; Tu et al. 2018; Garg and Biju 2019; Gorin et al. 2020) creates uncertainty which, along with the significant morphological disparity among G.molossus and the other species of Glyphoglossus examined (Parker et al. 1934), suggests that the generic taxonomy of the group may not be fully resolved.
Body size evolution in the Microhyla–Glyphoglossus assemblage
Among vertebrates, numerous clades of fishes, frogs, and squamate reptiles compete for the title of the smallest absolute body size, with several converging around body lengths (defined vastly differently in the three clades) of 8–12 mm (Hanken and Wake 1993). This apparent size limit has invoked the idea of physiological constraints preventing the evolution of smaller body sizes (Alexander 1996; Hedges and Thomas 2001; Scherz et al. 2019). As such, species exhibiting miniaturization provide interesting opportunities to understand the lower size limits of vertebrate physiology and development, whereas clades exhibiting miniaturized body plans offer opportunities to understand the dynamics of size evolution. Moreover, miniaturization is often associated with major morphological rearrangements (Hanken 1985; Hanken and Wake 1993; Polilov 2015), and is thought to have played a significant role in generation of some key innovations, such as the mammalian inner ear (Lautenschlager et al. 2018). It is therefore of great interest to also understand the consequences of miniaturization from a broad array of cases.
Frogs, and especially microhylids, have a particular propensity to miniaturize, with several microhylids in a variety of subfamilies achieving adult body sizes of 12 mm or smaller (Clarke 1996; Lehr and Coloma 2008; Das and Haas 2010; Rittmeyer et al. 2012; Rakotoarison et al. 2017; Scherz et al. 2019; Oliver et al. 2017). Despite this diversity, there are surprisingly few studies that have looked at miniaturization in a comparative context within the Microhylidae (e.g., de Sá et al. 2012, 2019b). Here, we have demonstrated that the Microhylinae are a particularly interesting clade of microhylids in which to study miniaturization, because they have converged repeatedly on extremely small body sizes.
Body size evolution in the Microhyla–Glyphoglossus was discussed in a study based on the maximum parsimony analysis of trait evolution, categorizing SVL into a series of bins (Gorin et al. 2020). Our analysis, which instead uses ancestral state reconstruction of continuous traits (Revell 2012) on our dated phylogeny, is largely congruent with that of Gorin et al. (2020) but provides better estimation of ancestral states and the timing of transitions in body size. Our results show clearly that this assemblage has undergone repeated miniaturization events, with Nanohyla miniaturizing first and independently from all Microhyla species; their most recent common ancestor is inferred to have been only a small frog (ca 25.3 mm in males). Within Microhyla, two large clades converged further toward the minimum size range, but six other lineages independently also became miniaturized (crossing the threshold of SVL < 20 mm). These replicates provide an opportunity to understand the relationship of certain morphological features with extreme body size reduction. Miniaturization of Nanohyla appears to have been coupled with the loss of metatarsal tubercles, whereas these are retained in even the smallest Microhyla. Likewise, the first finger of Nanohyla is often reduced to a nub, whereas it is always at least half the length of the second finger in Microhyla. This is reminiscent of the patterns seen in Stumpffia Boettger, 1881 frogs from Madagascar, where digit reduction is a hallmark of each major clade, and where the first finger is always the first to reduce (Rakotoarison et al. 2017). Unlike Stumpffia, however, even the smallest Microhyla and Nanohyla do not show reduction of the second and fourth fingers, although Microhylatetrix presents bizarre hand morphology with a particularly thick and long third finger (Poyarkov et al. 2020b) reminiscent of the third-finger-only phenotype seen in the smallest Stumpffia species. Also, they have not lost any phalanges, even when fingers are reduced in length, whereas other miniaturized frogs often show finger or toe formula reduction (Alberch and Gale 1983, 1985; Scherz et al. 2019). Still, there has been a tendency for the terminal phalanx of F1 to transition from T-shaped to knobbed to simple in miniaturization series, indicative of a strong reduction despite the lack of loss of this element.
In the vertebral column, Microhylanepenthicola (Fig. 6D) and Nanohylaarboricola (Fig. 6E) exhibit fusion of the first two presacral vertebrae, potentially linked to their extremely small body size. In the skull, both Nanohyla and Microhyla show forward displacement of the jaw articulation in miniaturized species, but other features are unique to each group, including the long otic ramus of the squamosal of Nanohyla (vs the reduction of the otic ramus of Microhyla), or the expansion of the sphenethmoid of Nanohyla along the parasphenoid (vs lack of expansion in Microhyla). The wide array of commonalities and differences both within these clades, and in comparison between these clades and other miniaturized frogs, highlights the extent to which miniaturization occurs through a combination of determinism and contingency. Nanohyla and Microhyla apparently share the reduction of the quadratojugal and loss of its connection to the maxilla, and the resulting take-over of suspensorium support by the pterygoid (Fig. 5). This arrangement is sometimes seen in other miniaturized microhylids (e.g., Anodonthylaeximia Scherz, Hutter, Rakotoarison, Riemann, Rödel, Ndriantsoa, Glos, Roberts, Crottini, Vences & Glaw [Scherz et al. 2019]), but, surprisingly, in the present case the loss of quadratojugal connection to the maxilla does not appear to be related to body size; even the largest species of Microhyla in the M.berdmorei group show the pterygoidal suspensorium support, but are not inferred to have passed through a period of extreme body size reduction that would be expected to result in such a degree of change. Thus, caution is always recommended when interpreting features as consequences of miniaturization, when they may have arisen through other selective pressures. Interestingly, some species within Nanohyla and Microhyla increased again in body size from an ancestral body size that was <18 mm. These species would be worthy of future investigation, because cases of post-miniaturization body-size increases can leave behind hallmarks (e.g., potentially irrevocable loss of anatomical features such as fingers), which can lead to morphological innovation (Hanken and Wake 1993).
Finally, although they are not miniaturized, it is worth briefly remarking on the osteology of Glyphoglossus, and especially the bizarre G.molossus. The osteology of G.yunnanensis is rather typical of a large-bodied microhylid, with long, slender limb bones and a subtriangular skull. Glyphoglossusmolossus, however, shows extreme osteological modification associated with its more fossorial lifestyle, from its thickened hind- and forelimb bones to its small, rounded skull, to its highly modified first presacral vertebra. The peculiar flattened snout in this species is formed by a large chondrified beard-looking structure, not co-ossified to rostral and mandibular bones. Its limb and skull modifications resemble other strong burrowers, e.g., Brevicepsgibbosus (Linnaeus, 1758) and Barygenysmaculata Menzies & Tyler (Menzies 2020; Van Dijk 2001).
Sexual size dimorphism in the Microhyla–Glyphoglossus assemblage
As is typical for frogs (Shine 1979), most members of the Microhyla–Glyphoglossus assemblage exhibit slight female-biased size dimorphism. There is a weak, but significant, positive correlation between log(male SVL) and sexual size dimorphism, with those species with the smallest males having the strongest female-biased size dimorphism, and dimorphism decreasing with increasing male SVL. They thus conform to Rensch’s rule (Rensch 1950). This may reflect a greater constraint on female body size, associated with the cost of reproduction in these frogs, which, even in the smallest species, produce clutches of many dozens of eggs.
Only a handful of species have transitioned, apparently rapidly and independently, to male-biased dimorphism. Remarkably, even species with diminutive males can be male-biased, exemplified by Microhyla sp. 1. In general, male-biased dimorphism is thought to be associated with territoriality and physical combat among male frogs (Shine 1979). These transitions to male-biased dimorphism may, therefore, be associated with changes in natural history of these lineages. Yet, this condition does not appear to be evolutionarily stable, because in no cases are a pair of sister species both male-biased. At present, too little is known of the ecology of these species to understand common drivers of these changes.
Conclusions
Miniaturized amphibians are characterized by a high proportion of cryptic species, along with numerous anatomical homoplasies, muddying our estimates of their evolutionary relationships and diversity (e.g., Hanken and Wake 1993; Rovito et al. 2013; Parra-Olea et al. 2016; Rakotoarison et al. 2017; Scherz et al. 2019; Gorin et al. 2020). Integrative taxonomic approaches, optimally combining the results of molecular phylogenetic analyses with morphological, acoustic and behavioral data, represent the most promising approach for better understanding of species boundaries, diversity and evolutionary relationships in microhylid frogs, including the genus Microhyla (Hasan et al. 2014; Garg et al. 2019; Poyarkov et al. 2018a, 2019; Gorin et al. 2020). Many recent phylogenetic studies of miniaturized frogs demonstrate that the diversity of these groups is unexpectedly high, at both the species and supraspecific levels, due to a combination of overlooked diversity (cryptic species) and microendemism (Oliver et al. 2017; Rakotoarison et al. 2017; Clemente-Carvalho et al. 2011; Poyarkov et al. 2018a; Zimkus et al. 2012; Blackburn et al. 2008; Lourenço-de-Moraes et al. 2018; Rodriguez et al. 2013; Köhler et al. 2008; Scherz et al. 2019). The present analysis of the Microhyla–Glyphoglossus assemblage diversity represents a case in point: miniaturized taxa, that were previously assigned ad hoc to Microhylas. lat., were demonstrated to belong to two deeply divergent clades, together closely related to the genus Glyphoglossus, which consists of species of a much larger body size and very different ecology. Upon closer examination of their phylogenetic relationships from molecular data, as well as morphology, ecology, and biogeography, we found that these deep clades were older than most other Microhylinae genera, and sufficiently different to justify recognition as distinct genera. This yielded the new genus Nanohyla gen. nov. described herein. This result further underlines the importance of genetic data, useful for independently elucidating diversity and evolutionary relationships within groups with extensive homoplasies (Mott and Vieites 2009; Heideman et al. 2011; Scherz et al. 2019).
The Microhyla–Glyphoglossus assemblage (perhaps better now called the Microhyla–Nanohyla–Glyphoglossus assemblage) shows highly dynamic body size evolution, and a propensity to miniaturize, with at least nine separate miniaturization events inferred across Microhyla and the new genus Nanohyla. Convergence in body size in these two genera has generated some homoplasies, but both have unique, apomorphic features. It is clear, however, that, in order to gain a comprehensive understanding of the evolution of miniaturization in these frogs, much more extensive sampling of outgroups is needed. The Microhylidae, however, form an ideal group in which to study the evolution of miniaturization, which is one of several phylogenetically recurring frog ecomorphs (Moen et al. 2015).
Acknowledgements
We express our sincere gratitude to V.F. Orlova, R.A. Nazarov, and E.A. Galoyan (ZMMU, Moscow, Russia) for permission to study specimens under their care. We deeply appreciate the help of those colleagues who provided various assistance, including specimens and materials for examination: C. Suwannapoom, E.N. Solovyeva, M. Hasan, H. Okamiya, D.M.S.S. Karunarathna, P. Pawangkhanant, A. de Silva, W. Juthong, E.A. Galoyan, K.D. Milto, L.T. Nguyen, A. Haas, D.P. Bickford, and I. Das. We are grateful to N.A. Formozov, A.N. Kuznetsov, A.A. Polilov, L.P. Korzun, V.V. Shakhparonov, I.B. Soldatova, and K.B. Gerasimov for many useful comments and suggestions on the discussion of our hypotheses and results. We thank A.A. Bannikova, I.V. Artyushin, A.B. Vassilieva, A.V. Diakova, S.E. Farisenkov, M.M. Perfilov, T.V. Duong, A.S. Dubrovskaya, P.V. Yushchenko, and S.S. Idiiatullina for valuable comments and help during our work on this project. We are deeply thankful to N.V. Kryukova who guided and helped us with making skeletal preparations and histological staining. We are grateful to D.R. Gafurova and A.N. Khomyak for assistance with micro-CT scanning. We thank V.D. Kretova who prepared line drawings for this study, and I. Das, P. Pawangkhanant, E. N. Solovyeva, V. D. H. Nguyen, L. T. Nguyen, T. V. Nguyen and vnherps.com for providing us with photos of several species of Microhyla and Nanohyla. We thank J. Mata, A. Resetar and the Field Museum of Natural History (Chicago, USA) for providing the photo of Microhylamaculifera. We also thank R. Brown and one anonymous reviewer for constructive feedback on this manuscript.
Funding
This work was supported by the Russian Foundation of Basic Research to Nikolay A. Poyarkov (RFBR grant No. 19-34-90167). Creation of dataset accessed on MorphoSource was made possible by the University of Florida (The oVert (openVertebrate) Thematic Collection Network (TCN); NSF DBI-1701714; NSF DBI-1702263). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Table S1
Microsoft Word Document (.docx)
Museum voucher information, geographic localities, and GenBank accession numbers of specimens and sequences used in this study. Asterisk (*) denotes sequences that were included in the alignment for timetree calibration. Exact locality information unknown for specimens obtained via pet trade or those published in earlier works.
https://binary.pensoft.net/file/496427This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl24451703071BF115-F0AF-56D6-96AA-3C6F013565A3
Table S2
Microsoft Word Document (.docx)
Museum voucher information and geographic localities of osteological specimens examined. For abbreviations see Materials and methods; SVL given in mm.
https://binary.pensoft.net/file/496428This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl34451705E69FB31D-E5E0-565C-A24D-4F310EFD1526
Table S3
Microsoft Word Document (.docx)
Body size data for the Microhyla–Glyphoglossus assemblage members (from Gorin et al. 2020, with modifications). For both sexes of each species maximal body size data is given. Question mark denotes “no data.” For voucher IDs of specimens see Gorin et al. (2020).
https://binary.pensoft.net/file/496429This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl44451707607BF66A-0375-5092-8CAD-2FD68A7E1678
Table S4
Microsoft Word Document (.docx)
Results of divergence time estimates. Node No. – estimated tree node, for node names see Suppl. material 8: Figure S3; divergence time given in million years before present (Ma).
https://binary.pensoft.net/file/496430This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl54451709D273FCE6-75B4-5E1C-AC35-0A4283DA5BC2
Table S5
Microsoft Word Document (.docx)
Osteological comparison of the Microhyla–Glyphoglossus assemblage members. For character definitions and state descriptions see Materials and methods; Latin numerals (I–VIII) refer to numbers of presacral vertebrae.
https://binary.pensoft.net/file/496431This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl644517113DD99456-287A-54C9-81EB-238A64512A4F
Figure S1
Adobe PDF file
Comparison of bayesian inference trees of the Microhyla–Glyphoglossus assemblage derived from the analysis of: (A) 2478 bp of mtDNA fragment including 12S rRNA, tRNAVal and 16S rRNA genes; (B) 720 bp of BDNFnuDNA gene; (C) the combined mtDNA + nuDNA dataset of 3207 bp including 12S rRNA, tRNAVal, 16S rRNA and BDNF gene fragments. For voucher specimen information and GenBank accession numbers see Suppl. material 1: Table S1. Yellow, red, and blue color denotes Microhyla I, Microhyla II, and Glyphoglossus, respectively. Numbers at tree nodes correspond to PP/BS support values, respectively.
https://binary.pensoft.net/file/496432This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl7445171363CD0A11-5045-5B8E-823B-FBE88F21368E
Figure S2
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Updated mtDNA-genealogy of the Microhyla–Glyphoglossus assemblage. For voucher specimen information and GenBank accession numbers see Suppl. material 1: Table S1. Yellow, red, and blue color denotes Microhyla I, Microhyla II, and Glyphoglossus, respectively. Numbers at tree nodes correspond to PP/BS support values, respectively.
https://binary.pensoft.net/file/496433This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl844517175E590428-0560-52F8-855F-622F79F4B593
Figure S3
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Bayesian chronogram resulted from *BEAST analysis of the 3207 bp-long concatenated mtDNA + nuclear DNA dataset. Node values correspond to node numbers, for estimated divergence times (in Ma) see Suppl. material 4: Table S4. Red circles correspond to calibration points used in molecular dating analysis, for details see Gorin et al. (2020). Blue bars correspond to 95%-confidence intervals.
https://binary.pensoft.net/file/496434This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl94451719461A741B-C9A1-53D2-8849-1D7B53A449A4
Figure S4
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Hand preparations of the three representatives of the Microhyla–Glyphoglossus assemblage. Pictures provided for G.guttulatus (A – dorsal view of the skull, B – lateral view of the skull, palmar view of the hand), M.fissipes (D – dorsal view of the skull, E – lateral view of the skull, F – palmar view of the hand) and N.marmorata (G – dorsal view of the skull, H – lateral view of the skull, I – palmar view of the hand).
https://binary.pensoft.net/file/496435This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov10.3897/zse.97.57968.suppl1044517150055880E-C6DE-5C47-B64D-54A6DB8627DF
Figure S5
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Variable states of osteological characters in the Microhyla–Glyphoglossus assemblage. (A) mineralized prehallux of M.butleri; (B) cartilaginous prehallux of N.annamensis; (C) ossified prehallux of G.guttulatus; (D) fan-shaped sternum of M.picta; (E) bifurcate sternum of M.nilphamariensis, (F) pectoral girdle of M.annectens (omosternum shown by an arrow); (G, H, I) – mineralized stapes of N.pulverata, M.berdmorei and miniaturized M.minuta (shown with an arrow) respectively; (J, K, L) – vertebral column of G.guttulatus, M.fissipes and N.marmorata respectively; (M) – hyoid of M.okinavensis; (N, O) – palatine region of N.marmorata and M.fissipes respectively (neopalatine shown with an arrow).
https://binary.pensoft.net/file/496436This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.Vladislav A. Gorin, Mark D. Scherz, Dmitriy V. Korost, Nikolay A. Poyarkov