13urn:lsid:arphahub.com:pub:C9EFD5EB-E909-52A5-90B8-2C7119603A4Eurn:lsid:zoobank.org:pub:ED34F394-2E4C-49D6-8300-0DC18F233E6CZoosystematics and EvolutionZSE1435-19351860-0743Pensoft Publishers10.3897/zse.95.3448634486Research ArticleGastropodaInvertebrataMolluscaSystematicsAsiaAustralasiaA glimpse in the dark? A first phylogenetic approach in a widespread freshwater snail from tropical Asia and northern Australia (Cerithioidea, Thiaridae)BoonmekamDusit1KrailasDuangduenhttps://orcid.org/0000-0002-6196-42981GimnichFrance2NeiberMarco T.mneiber@hotmail.de3GlaubrechtMatthias3Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, ThailandSilpakorn UniversityNakhon PathomThailandZoologisches Forschungsmuseum Alexander Koenig, Adenauerallee 160, 53113 Bonn, GermanyZoologisches Forschungsmuseum Alexander KoenigBonnGermanyCenter of Natural History (CeNak), Universität Hamburg, Martin Luther King-Platz 3, 20146 Hamburg, GermanyUniversität HamburgHamburgGermany
Corresponding author: Marco T. Neiber (marco-thomas.neiber@uni-hamburg.de)
Academic editor: Thomas von Rintelen
201903072019952373390FF989B37-9851-366F-FF9F-4769FFC5FE53BC529DE5-DF53-41EF-A803-383E08E0472133224011203201930052019Dusit Boonmekam, Duangduen Krailas, France Gimnich, Marco T. Neiber, Matthias GlaubrechtThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://zoobank.org/BC529DE5-DF53-41EF-A803-383E08E04721
Thiaridae are a speciose group of freshwater snails in tropical areas including a high number of described nominal taxa for which modern revisions are mostly lacking. Using an integrative approach, the systematic status of a group of thiarids from the Oriental region, including the nominal species Melaniaaspera and M.rudis, is reassessed on the basis of shell morphology and biometry, radula dentition patterns, and reproductive biology along with molecular genetic methods. Our results suggest that populations from the Oriental region cannot be distinguished on the basis of shell morphology, radula characters and their reproductive mode and are monophyletic based on mitochondrial sequences. Hence, M.rudis with M.aspera are regarded as belonging to the same species along with several other nominal taxa that were previously included in M.rudis. Moreover, populations from Thailand and Australia, from where the species was not previously recorded, could be shown to form a monophyletic group together with samples from Indonesia. However, a generic affiliation with Thiara, in which the investigated taxa were often included in the past, was not supported in our phylogenetic analyses, highlighting the need for a comprehensive revision of the genus-group systematics of Thiaridae as a whole.
Despite advances in the understanding of the family-level phylogeny of Cerithioidea Fleming, 1822, the taxonomical diversity in Thiaridae Gill, 1871 (1823) is still not well understood, and evolutionary systematic research in the sense of Glaubrecht (2010) in this particular family is still in its infancy. The Thiaridae, in earlier treatments subsumed under the name Melaniidae Children, 1823, have been used as a “rubbish bin” to accommodate all freshwater lineages belonging to the Cerithioidea. Only after the removal of the families Pachychilidae Fischer & Crosse, 1892, Melanopsidae Adams & Adams, 1854, Paludomidae Stoliczka, 1868, Pleuroceridae Fischer, 1885 (1863), and Semisulcospiridae Morrison, 1952) (Campbell 2019; Neiber and Glaubrecht 2019b, 2019c, 2019d; Strong and Lydeard 2019 and references therein) and recently the Neotropical Hemisinidae Fischer & Crosse, 1891 (Glaubrecht and Neiber 2019a), a more accurate circumscription of “core” Thiaridae began to emerge on the basis of molecular and/or morphological evidence (e.g., Glaubrecht 1993, 1996, 2011; Holznagel and Lydeard 2000; Lydeard et al. 2002; Glaubrecht et al. 2009; Strong 2011; Strong et al. 2011).
In addition to uncertainties in the delimitation of genera, research on thiarids is further complicated by the large disparity of shell characters among species, a large phenotypic plasticity within species and a high ecological adaptability that is, however, also known from other limnic Cerithioidea. This conchological variability has certainly led to an overestimation of the number of species in the past, as specifically shown for limnic lineages in the superfamily (Glaubrecht 1993, 1996; Köhler and Glaubrecht 2001, 2003, 2006; Glaubrecht and Köhler 2004; Glaubrecht et al. 2009), but may also cause problems in delimiting species resulting in an underestimation of the actual morphological disparity versus the taxonomical diversity, at least in some cases. These problems are exacerbated by the putatively widespread occurrence of parthenogenesis in different lineages of Thiaridae (Glaubrecht 1996) and the associated problems of what is actually meant by “species” in this case (e.g. Hausdorf 2011). Additionally, Thiaridae have also realised different life history strategies that were characterised by Glaubrecht (1996, 1999, 2006, 2011) by the duration of ontogenetic stages to remain within a specialised structure of the female, viz. the subhaemocoelic brood pouch. While in some thiarids only very early ontogenetic stages, i.e. embryos without shell, develop and are released as veligers (ovoviviparity), other thiarid species brood and even transform their subhaemocoelic brood pouch into a matrotrophic organ or “pseudoplacenta” that apparently nourishes the developing juveniles, as e.g. in the Southeast Asian thiarid Tarebiagranifera (Lamarck, 1816) (euviviparity, see Glaubrecht 1996; Glaubrecht et al. 2009; Maaß and Glaubrecht 2012; Veeravechsukij et al. 2018b). Finally, some thiarids also have an extraordinarily high invasive potential, such as Melanoidestuberculata (Müller, 1774) and Tarebiagranifera and today have an almost pantropical distribution (e.g., Brown 1994; Glaubrecht 1996).
To date, only few of the several dozen thiarid taxa have seen closer investigation. Glaubrecht et al. (2009) and Maaß and Glaubrecht (2012) surveyed the thiarid fauna of Australia. Dechruska et al. (2013) evaluated the status and identity of the nominal taxon Melaniajugicostis Hanley & Theobald, 1876 from the Southeast Asian mainland, and Veeravechsukij et al. (2018a, 2018b) investigated the phylogeography and reproductive biology of T.granifera and its trematode parasites. However, many other named taxa have been rarely studied and, thus, remain enigmatic and even pure nomenclatorial “ghosts” with highly questionable status as evolutionary relevant entities, which hampers further insights into the systematics, biogeography, and evolution of these freshwater gastropods otherwise under scrutiny, e.g., in speciation and/or radiation studies.
Melaniaaspera Lesson, 1831, which was originally described from New Guinea (Lesson 1830–1831), is such an “enigmatic” taxon (Fig. 1), which Glaubrecht and Podlacha (2010) regarded as a possible senior synonym of the nominal species Melaniarudis Lea & Lea, 1851. The latter taxon is usually regarded as belonging to Thiara Röding, 1798 and thought to be relatively widespread, being reported from several countries, occurring from India and Sri Lanka to Southeast Asia and the Indo-Australian archipelago (Schepman 1892, 1915; Rensch 1934; van Benthem Jutting 1937; Subba Rao 1989; Ramakrishna and Dey 2007; Budha 2010; Patil and Talmale 2011, see also Fig. 2). However, actual distribution records are relatively scarce in the literature and the distinction from other nominal thiarid taxa remains uncertain so far.
Shells of “Thiara” aspera (Lesson, 1831). A. Holotype of Melaniaaspera Lesson, 1831, MNHN 21098, ‘La Nouvelle-Guinée’ [more specifically Manokwari on New Guinea Island, West Papua, Indonesia, see Glaubrecht and Podlacha 2010]; B. Syntype of Melaniarudis Lea & Lea, 1851, USNM 119778, Amboyna; C. Syntype of Melaniamicrostoma Lea & Lea, 1851, USNM 119722, mountain streams, isle of Negros, Philippines; D.ZMB 107002, Calcutta, India; E.ZMB 107003, Ceylon, Sri Lanka; F.ZMB 127534, Don Ko Canal, Nakhon Pathom, Thailand; G.ZMB 127535, Don Ko Canal, Nakhon Pathom, Thailand; H.ZMB 127535, Don Ko Canal, Nakhon Pathom, Thailand; I.ZMB 191279, Yehembang River, Bali, Indonesia; J.ZMB 191279, Yehembang River, Bali, Indonesia; K.ZMB 106472, Yehembang, Bali, Indonesia; L. East of Mendaya, stream southwest of Gumicik, Bali, Indonesia; M.ZMB 191278, stream at Tembeeha, road Tirobus-Kendari, Southwest Sulawesi, Indonesia; N.ZMB 107378, Banggai Islands, Peleng Island, West of Peninsula, Tataban river, Central Sulawesi, Indonesia; O.ZMB 107377, Banggai Islands, Peleng Island, West of Peninsula, Tataban river, Central Sulawesi, Indonesia.river; P, Q.ZMB 107617, Wabalarr, Roper River, Northern Territory, Australia; R.ZMB 106599, Berry Springs, Northern Territory, Australia. Scale bar: 1 cm.
Distribution and reproductive strategy of “Thiara” aspera (Lesson, 1831). Stars: type localities of a) Melaniaaspera Lesson, 1831, Monokwari, New Guinea, b) Melaniarudis Lea & Lea, 1851, Amboyna and c) Melaniamicrostoma Lea & Lea, 1851, mountain streams, isle of Negros, Philippines. Pie charts show the percentages of offspring in the brood pouch of female T.aspera in different size classes as defined in Glaubrecht et al. (2009), see inset. The numbers near the pie charts refer to the number of individuals examined per population. Filled circles: material preserved in ethanol; open circles: dry shells.
https://binary.pensoft.net/fig/314777
As a further contribution towards a better understanding of thiarid diversity, we here re-evaluate the identity of M.aspera and M.rudis on the basis of museum samples including available type material as well as material collected during ongoing surveys in Southeast Asia using shell morphology and biometry, radula dentition patterns, and reproductive biology along with molecular genetic methods. Nomenclatural issues and the synonymy of the genus Thiara are also discussed.
Material and methods
This study is mainly based on the examination of specimens in the collections of the Parasitology and Medical Malacology Research Unit, Department of Biology, Faculty of Science, Silpakorn University, Thailand and the Museum für Naturkunde, Berlin, Germany, and supplemented by material from other museums (see below). Additionally, new samples were collected using hand picking and scooping methods in Thailand and Australia. Specimens were fixed in 75–96% ethanol.
Collection acronyms
MNHNMuséum National d’Histoire Naturelle de Paris, France
SUT Silpakorn University, Nakhon Pathom, Thailand
USNMNational Museum of Natural History, Washington, USA
ZMBMuseum für Naturkunde, Berlin, Germany (formerly Zoologisches Museum Berlin)
Coordinates (WGS84) of localities were taken with a GPS device or determined as accurately as possible from a map. Sampling sites were then mapped on a dot-by-dot basis to a digitally reduced version of the drainage pattern map of the Indo-Australian region. This map was prepared using a relief map on the basis of the Global30-Arc-Second Elevation Data (GTOPO30) from the U.S. Geological Survey and a river map from the map server Aquarius Geomar; and then compiled using Adobe Photoshop CS3 and Adobe Illustrator. For the exact locality data, see the material examined section.
Shell characters
Specimens were photographed using a digital EOS 350D camera (Canon, Tokyo, Japan). Standard biometric parameters were taken from each shell using electronic callipers (accuracy 0.1 mm): shell height (H), shell width (W), aperture length (AL; measured from the upper apertural angle to the farthest point on the basal margin of the aperture), aperture width (AW; measured perpendicular to AL as the widest distance between outer apertural margin and outer margin of parietal callous), height of the body whorl (BW), and number of whorls (NW) as shown in Figure 3A. To reduce dimensionality a principal component analysis was conducted on log-transformed shell measurements using R 3.3.2 (R Core Team 2016). Only the minimal number of PCA axes that accounted for more than 95% of the cumulative variation were used for further testing.
Measured shell parameters. A: H – shell height; W – shell width; BW – body whorl height; AL – aperture length; AW – aperture width. B: he – height of embryonic shell; we – width of embryonic shell; de – maximum diameter at one whorl.
https://binary.pensoft.net/fig/314778
The Shapiro-Wilk test was conducted in R to test for normal distributions of PCA 1 and PCA 2 values, respectively, for the here proposed geographic subgroups, i.e., samples from 1) Thailand, 2) Indonesia, 3) India, and Sri Lanka, and 4) Australia. Since some of the Shapiro-Wilk tests were significant (p ≤ 0.05), the non-parametric Kruskal-Wallis rank sum test was conducted for PCA 1 and PCA 2 assuming the grouping of specimens according to geography followed by Dunn’s test (Bonferroni-corrected) as post-hoc test as implemented in the R package “dunn.test 1.3.5” (Dinno 2017) in case that the Kruskal-Wallis-rank-sum tests were significant.
Radula preparation
Shells of representative specimens were cracked with a small vice and removed from the soft body parts, which were afterwards examined and dissected with the aid of a Leica Wild MZ 9.5 stereo microscope (Leica Microsystems, Wetzlar, Germany). Radulae were extracted following the protocol of Holznagel (1998), fixed on aluminium stubs, and coated with platinum using a Polaron SC 7640 Sputter Coater (Quorum Technologies, East Grinstead, UK). Radulae were then viewed and photographed (oriented so that denticles on the teeth were well visible) with a scanning electron microscope (SEM) EVO LS10 (Zeiss, Oberkochen, Germany).
Content of brood pouch
The brood pouch was opened after removing the mantle and its content was counted under a Leica Wild MZ 9.5 stereo microscope. Both, shelled juveniles and embryos, were grouped into standard size classes as described in Glaubrecht et al. (2009). Embryos and juveniles from representative specimens were fixed on aluminium stubs, air-dried, coated with platinum using a Polaron SC 7640 Sputter Coater, and then viewed, photographed, and measured (Fig. 3B) with a EVO LS10 SEM. Parameters of the embryonic shell were measured from SEM images as shown in Figure 3B: diameter of first half whorl (de; measured as the maximal witdth of the shell after 0.75 turns of the suture line), width of first quarter whorl (he; measured parallel to de as the distance from the starting point of the suture to the point after 0.25 turns of the suture line), width of first half whorl (we; measured perpendicular to de as the distance from the starting point of the suture to the point after 0.5 turns of the suture line).
Molecular methods and phylogenetic analyses
Total genomic DNA was extracted from ethanol-preserved foot muscle tissue using a CTAB protocol as described by Winnepenninckx et al. (1993) from 31 thiarid specimens and Paludomussiamensis Blanford, 1903 as outgroup representing one of the cerithioidean families, which have been shown to be closely related to the Thiaridae (Wilson et al. 2004; Strong et al. 2011).
For phylogenetic analyses, fragments of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the 16 S rRNA (16S) gene were amplified by polymerase chain reaction (PCR) using the primer pairs LCO1490 (5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’; Folmer et al. 1994) plus HCO2198var (5’-TAW ACT TCT GGG TGK CCA AAR AAT-3’; Rintelen et al. 2004) and 16S_F_Thia2 (5’-CTT YCG CAC TGA TGA TAG CTA G-3’; Neiber and Glaubrecht 2019a, see also Gimnich 2015) plus H3059var (5’-CCG GTY TGA ACT CAG ATC ATG T-3’; Wilson et al. 2004), respectively. Amplifications were conducted in 25 µl volumes containing 50–100 ng DNA, 1× PCR buffer, 200 mM of each dNTP, 0.5 mM of each primer and 1 U of Taq polymerase. After an initial denaturation step of 3 min at 94 °C, 35 cycles of 30 s at 94 °C, 60 s at 45–62°C and 60–120 s at 72°C were performed, followed by a final extension step of 5 min at 72°C. PCR products were purified using a NucleoSpin Extract II Kit (Macherey–Nagel, Bethlehem, PA, USA). Both strands of the amplified gene fragments were cycle-sequenced using the primers employed in PCR with the Big Dye Terminator chemistry version 1.1 (Applied Biosystems, Inc., Waltham, MA, USA). Sequences were visualised on an ABI 3130xl or ABI 3730xl Genetic Analyzer (Applied Biosystems, Inc.).
Forward and reverse sequence reads were assembled with CODONCODE ALIGNER v. 3.7.1 (CodonCode Corporation, Dedham, MA, USA) and corrected by eye. For information on vouchers, see Table 1. The protein coding cox1 sequences were aligned with MUSCLE (Edgar 2004) as implemented in MEGA 6 (Tamura et al. 2013) under default settings. The 16S sequences of were aligned with MAFFT (Katoh and Standley 2013) using the Q-INS-i iterative refinement algorithm and otherwise default settings, because this algorithm has been described to perform better for the alignment of sequence data sets that may contain deletions and insertions than alternative multiple sequence alignment methods (Golubchik et al. 2007).
Museum registration numbers, GenBank accession numbers and locality data for the specimens used in the molecular phylogenetic analyses. Abbreviations for countries: AUS – Australia, IDN – Indonesia, IND – India, THA – Thailand.
Taxon
Museum number
Extraction number
Country
Latitude
Longitude
GenBank accession number
cox1
16 S rRNA gene
“Thiara” aspera
SUT 0311020
11449
THA
13°38'08"N, 100°05'03"E
MK879291
MK879427
SUT 0312070
11446
THA
13°48'08"N, 100°02'06"E
MK879292
MK879428
SUT 0311044
9603
THA
13°38'08"N, 100°05'03"E
MK879290
–
ZMB 191268
2200
IDN
03°39'28"S, 122°13'52"E
MK879296
MK879434
ZMB 191488
4558
IDN
08°38'39"S, 115°16'38"E
MK879297
MK879435
ZMB 107377
6494
IDN
01°32'18"S, 122°51'28"E
MK879293
MK879429
ZMB 107378
6495
IDN
01°32'18"S, 122°51'28"E
MK879294
MK879430
ZMB 107617
7586
AUS
14°56'02"S, 133°10'26"E
MK879295
MK879433
ZMB 107617
8743
AUS
14°56'02"S, 133°10'26"E
–
MK879431
ZMB 107617
8744
AUS
14°56'02"S, 133°10'26"E
–
MK879432
“Stenomelania” denisoniensis
ZMB 106682
7599
AUS
14°55'47"S, 133°08'44"E
MK879288
MK879425
ZMB 106632
7602
AUS
15°00'42"S, 133°14'25"E
MK879287
MK879424
Thiaraamarula
ZMB 191489
2886
IDN
01°26'43"S, 127°29'01"E
MK879289a
MK879426a
ZMB 107472
6496
IDN
03°35'28"S, 128°08'42"E
MK094074
MK098355
Thiarawinteri
ZMB 106554
1043
IDN
08°23'38"S, 114°45'04"E
MK879301
MK879439
ZMB 190261
1055
IDN
02°35'34"S, 120°54'10"E
MK879302
MK879440
Thiaracf.winteri
ZMB 106472
1001
IDN
08°23'38"S, 114°45'04"E
MK879298
MK879436
ZMB 191279
2232
IDN
08°23'36"S, 114°45'04"E
MK879299
MK879437
ZMB 191279
4559
IDN
08°23'36"S, 114°45'04"E
MK879300
MK879438
Mieniplotiascabra
ZMB 107382
6514
IDN
00°48'33"N, 127°17'40"E
MK879279
MK879416
ZMB 107564
7340
AUS
14°55'38"S, 133°07'06"E
MK879280
MK879417
ZMB 127495
9574
THA
07°55'15"N, 099°15'47"E
MK879285
MK879422
SUT 0312060
9578
THA
12°51'15"N, 099°59'49"E
MK879278
MK879415
SUT 0311024
9580
THA
14°54'04"N, 100°03'48"E
MK879276
MK879413
SUT 0311040
9582
THA
13°25'07"N, 099°57'18"E
MK879277
MK879414
ZMB 127470
9589
THA
08°27'09"N, 098°28'01"E
MK879284
MK879421
ZMB 127468
9599
THA
12°56'54"N, 099°28'52"E
MK879283
MK879420
ZMB 107962
9779
THA
16°37'38"N, 100°56'43"E
MK879282
MK879419
ZMB 107869
9781
THA
08°38'18"N, 099°44'59"E
MK879281
MK879418
SUT 0311009
9787
THA
16°11'33"N, 099°15'51"E
MK879275
MK879412
Melanoidestuberculata
ZMB 200313
7530
IND
11°34'45"N, 076°34'55"E
MK879274
MK879411
Paludomussiamensis
ZMB 107721
7334
THA
14°26'15"N, 098°51'11"E
MK879286
MK879423
a From Neiber and Glaubrecht (2019a).
Maximum likelihood (ML), Bayesian Inference (BI), and maximum parsimony (MP) approaches were used to reconstruct the phylogenetic relationships. The sequence data set was initially divided into four partitions for the nucleotide model-based ML and BI approaches: 1) 1st codon positions of cox1, 2) 2nd codon positions of cox1, 3) 3rd codon positions of the cox1, and 4) the 16S. To select an appropriate partitioning scheme and evolutionary models the sequence data set was analysed with PARTITIONFINDER v. 1.1.1 (Lanfear et al. 2012) conducting an exhaustive search and allowing for separate estimation of branch lengths for each partition using the Bayesian information criterion as recommended by Luo et al. (2010). Models to choose from were restricted to those available in MRBAYES v. 3.2.6 (Ronquist et al. 2012) as well as in GARLI v. 2.1 (Zwickl 2006). As best-fit partitioning scheme, the PARTITIONFINDER analysis suggested to combine the 1st and 2nd codon positions of cox1 and the 16S sequences together in one partition (GTR + G model) and the 3rd codon positions of cox1 in a second partition (HKY + G model).
The BI analysis was performed using MRBAYES v. 3.2.6. Metropolis-coupled Monte Carlo Markov chain (MC3) searches in MRBAYES were run with four chains in two separate runs for 50,000,000 generations with default priors, trees and parameters sampled every 1000 generations under default heating using the best-fit model as suggested by PARTITIONFINDER. Diagnostic tools in MRBAYES, including Estimated Sample Size (ESS) values > 200, were used to ensure that the MC3 searches had reached stationarity and convergence. The first 5,000,000 generations of each run were discarded as burn-in.
Heuristic ML analysis was performed with GARLI using the best-fit models as suggested by PARTITIONFINDER. Support values were computed by bootstrapping (BS) with 1,000 replicates.
Heuristic MP searches were carried out with PAUP v. 4.0b10 (Swofford 2002) using 100 random-addition-sequence replicates and TBR branch swapping. Support values were computed by bootstrapping with 1,000 replications.
Alternative phylogenetic hypotheses were tested using the approximately unbiased (AU) test (Shimodeira 2002) as implemented in the program CONSEL (Shimodeira and Hasegawa 2001). Information on vouchers and GENBANK accession numbers are listed in Table 1.
ResultsBiometric analyses
The first two principal components (PCA 1 and PCA 2) account for > 95% of the cumulative variation in shell parameters. The plot of PCA 1 vs PCA 2 (Fig. 4A) shows that the clusters of specimens that were grouped according to geographic origin widely overlap. Especially the clusters of specimens from Thailand and Indonesia (corresponding to mitochondrial Clades A and B, Fig. 4) and the clusters of specimens from Australia (corresponding to mitochondrial Clade C, Fig. 4) also widely overlap. The Kruskal–Wallis rank sum tests were significant for PCA 1 (p < 5.0 × 10−6) and PCA 2 (p < 2.0 × 10−16), i.e., at least one group stochastically dominates one other group in each of the tests. Dunn’s test for PCA 1 found significant differences between the groups including samples from Indonesia and Australia (p < 0.0001) as well as between the groups including samples from Australia and Thailand (p < 0.0075), respectively, but not for pairwise comparisons of the other groups (Fig. 4B). Dunn’s test for PCA 2 found significant differences between the following groups: Indonesia vs Australia (p < 0.0031), Indonesia vs Thailand (p < 0.0001), Australia vs Thailand (p < 0.0007), Australia vs India/Sri Lanka (p < 0.0004), and Thailand vs India/Sri Lanka (p < 0.0001), but not for Indonesia vs India/Sri Lanka (Fig. 4C). However, both for PCA 1 and PCA 2 the comparison of ranges shows that the ranges of all pairs of geographic groups overlap and therefore do not allow a diagnostic separation of these groups on the basis of the biometric data. The included type specimens of the nominal taxa M.rudis and M.microstoma fall within the convex hull spanned by specimens sampled from Thailand, Indonesia, Australia, India, and Sri Lanka in the PCA 1 vs PCA 2 plot; only the holotype of the nominal taxon M.aspera lies outside this area (Fig. 4A), although closely resembling the examined syntypes of M.rudis and M.microstoma with respect to shell sculpture and overall shape.
Results of the analysis of biometric data of “Thiara” aspera (Lesson, 1831) specimens from Australia (yellow), Indonesia (green), Thailand (red) and India/Sri Lanka (blue) and type material of Melaniaaspera Lesson, 1831 (holotype, triangle), Melaniarudis Lea & Lea, 1851 (syntype, square) and Melaniamicrostoma Lea & Lea, 1851 (syntype, diamond). A. Scatter plot of the first two axes of the principal component analysis (PCA) of biometric data. Coloured lines indicate the outline of the convex hull for each geographic group; B, C. Boxplots of PCA 1 (B) and PCA 2 (C); bars above the box plots indicate significant differences of groups resulting from testing with Dunn’s test.
Bayesian 50% majority-rule consensus tree based on partial sequences mitochondrial cytochrome c oxidase subunit 1 (cox1) and 16S rRNA (16S) genes. Support values at nodes refer to Bayesian posterior probabilities (left), Maximum Likelihood (middle) and Maximum Parsimony (right) bootstrap values. AUS: Australia, IDN: Indonesia, THA: Thailand. Numbers at tips refer to DNA vouchers in the collection of the ZMB, see also Table 1.
A clade including Thiaraamarula (Linnaeus, 1758) (the type species of Thiara Röding, 1798), T.winteri (Busch, 1842) in Philippi (1842–1844), T.cf.winteri from Bali, and the specimens identified as Thiaraaspera from Thailand, Indonesia, and Australia as well as “Stenomelania” denisoniensis (Brot, 1877) in Brot (1874–1879) was recovered in all three analyses (BI: 1.00, BS (ML): 92, BS (MP): 96). However, Thiara is paraphyletic with respect to “S.” denisoniensis. Thiaraamarula grouped together with T.winteri and T.cf.winteri from Bali in a clade (BI: 1.00, BS (ML): 97, BS (MP): 93). A sister group relationship of T.amarula and T.winteri was recovered in the BI and ML analyses (BI: 0.99, BS (ML): 83) but not in the MP analysis (BS (MP): < 50). Within this clade, the clades including specimens of T.amarula, T.winteri, and T.cf.winteri from Bali, respectively, were supported (BI: 1.00, BS (ML): 96–100, BS (MP): 100). The clade containing T.amarula, T.winteri, and T.cf.winteri was recovered as the sister group of a clade containing “S.” denisoniensis and specimens from Thailand, Australia, and Indonesia (including also a single specimen from Bali) assigned to the T.aspera on basis of conchological similarity with rather high support (BI: 1.00, BS (ML): 92, BS (MP): 96). Within this clade, “S.” denisoniensis was recovered as the sister group (BI: 1.00, BS (ML): 94, BS (MP): 90) of T.aspera, which in turn formed a rather well-supported clade (BI: 1.00, BS (ML): 90, BS (MP): 79). Thiaraaspera specimens from Australia (Clade C) formed a maximally supported clade. The T.aspera specimens from Thailand grouped together with a single individual from Bali in a well-supported clade (Clade A; BI: 1.00, BS (ML): 92, BS (MP): 100), which was sister to another supported clade (Clade B; BI: 1.00, BS (ML): 77, BS (MP): 100) that included T.aspera specimens from Sulawesi.
The included specimens of Mieniplotiascabra (Müller, 1774) formed the sister clade of a specimen of Melanoidestuberculata from India in the BI analysis, albeit without support. To test alternative phylogenetic hypotheses, we conducted four AU tests: 1) the monophyly of M.scabra (p = 0.252) and 2) the monophyly of T.winteri plus the T.cf.winteri specimens from Bali (p = 0.156) could not be rejected, whereas 3) the monophyly of T.aspera, T.winteri and T.cf.winteri (p < 0.001) and 4) the monophyly of Thiara excl. “S.” denisoniensis (p = 0.033) but including the T.aspera specimens was rejected at a confidence level of α = 0.05.
Systematic accountThiaridae Gill, 1871 (1823)AnimaliaSorbeoconchaThiaridae37090076-8718-5514-851A-38AAB0FF7971ThiaraRöding, 1798*VesicaHumphrey, 1797: 58 [unavailable, published in a work rejected for nomenclatural purposes, see International Commission on Zoological Nomenclature 1912: 116–117; among the mentioned species is Vesicathiara Humphrey, 1797 (unavailable) = Helixamarula Linnaeus, 1758].ThiaraRöding, 1798: 109 [type species: Helixamarula Linnaeus, 1758, by subsequent designation of Herrmannsen 1849 in Herrmannsen 1847–1849: 576].MelaniaLamarck, 1799: 75 [type species: Helixamarula Linnaeus, 1758, by monotypy].MelanigenusRenier, 1807: pl. 8 [unavailable, published in a work rejected for nomenclatural purposes, see International Commission on Zoological Nomenclature 1956: 290].MelasMontfort, 1810: 322–324 [unjustified emendation of Melania Lamarck, 1799].MelanidiaRafinesque, 1815: 144 [unjustified emendation of Melania Lamarck, 1799].Melanea– Sowerby 1818 in Sowerby 1818–1822: 33 [incorrect subsequent spelling of Melania Lamarck, 1799].? Spirilla Gray, 1824: 254 [unavailable, published in synonymy; mentioned as Spirillaspinosa (quoting a label or note attributed to G. Humphrey as “Spirillaspinosa, freshwater spiral spined shell, from Admirality Island, New Guinea”) under Melaniasetosa Swainson, 1824 (= Thiaracancellata Röding, 1798, see Swainson 1824: 13–15 and Wilkins 1957: 167–169) and as being conspecific with the nomenclaturally unavailable Buccinumaculeatum Lister, 1692: pl. 1055, fig. 8. Mentioned as a synonym by Férussac 1824: 318, Gray 1825: 524, Oken 1833: 133, Gray 1847: 152, Wilkins 1957: 167 as well as by Agassiz 1842: 84, Agassiz 1847: 348 and Herrmannsen 1848 in Herrmannsen 1847–1849: 491 in nomenclators, with the name attributed to Humphrey 1797 (where it could not be found). Used by Favre 1869: 79 (attributing the name to G. Humphrey but without reference to the work of Gray 1824) for a subgenus of Fusus Bruguière 1789 in Bruguière 1789–1792 and in a very different meaning from that of Gray 1824 and therefore not regarded here as having been made available from that work]. Spirella– Oken 1833: 61 [incorrect subsequent spelling of the unavailable Spirilla Gray, 1824].MelacanthaSwainson, 1840: 341 [type species: Helixamarula Linnaeus, 1758 by subsequent designation of Herrmannsen 1849 in Herrmannsen 1847–1849: 26].Thaira– Gray 1840: 148 [incorrect subsequent spelling of Thiara Röding, 1798].AmarulaSowerby, 1842: 61 [type species: Helixamarula Linnaeus, 1758, by monotypy].Melanium– Busch 1842 in Philippi 1842–1845: 4 [incorrect subsequent spelling of Melania Lamarck, 1799].Tiara– Gray 1847: 152 [incorrect subsequent spelling of Thiara Röding, 1798].Thaera– Agassiz 1847: 367 [unavailable, emendation for Thaira as used by Gray 1840: 148 proposed in synonymy in a nomenclator].LithoparchesGistel, 1848: ix [nom. nov. pro Melania Lamarck, 1799; type species: Helixamarula Linnaeus, 1758, by typification of the replaced name].HydrognomaGistel, 1848: 169 [nom. nov. pro Melania Lamarck, 1799, type species: Helixamarula Linnaeus, 1758, by typefication of the replaced name].TiaropsisBrot, 1871: 298 [non Agassiz 1849: 289–298; type species: Melaniawinteri Busch, 1842 in Philippi 1842–1844: Melania, 1, pl. 1 figs 1, 2 by subsequent designation of Brot 1874 in Brot 1874–1879: 7].CerithomelaniaMoore, 1899: 233–234 [type species: Helixamarula Linnaeus, 1758 by original designation].? Ripalania Iredale, 1943: 209 [type species: Melaniaqueenslandica Smith, 1882 by monotypy]. ? Setaeara Morrison, 1952: 8 [type species: Thiaracancellata Röding, 1798 by original designation]. Remarks.
Many names have been proposed for the group of Thiaridae that is currently regarded as representing Thiara Röding, 1798. Several of these names are objective junior synonyms of Thiara having the same type species (Helixamarula Linnaeus, 1758), and several others are nomenclaturally unavailable. A few, like Ripalania Iredale, 1943 or Setaeara Morrison, 1952, may actually be synonyms of Thiara. However, those hypotheses should be further tested using molecular genetic approaches. Therefore, these nominal genera were only tentatively included in the synonymy of Thiara.
AnimaliaSorbeoconchaThiaridae2862B7A0-CC0B-5EF8-81AE-5E13A796EC9C“Thiara”aspera (Lesson, 1831)*Figs 1, 6, 7MelaniaasperaLesson, 1831 in Lesson (1830–1831: 357–358) [type locality: “La Nouvelle-Guinée” (= New Guinea), restricted to Manokwari by Glaubrecht and Podlacha (2010)].MelaniarudisLea & Lea, 1851: 186 [type locality: ‘Amboyna’ (= Ambon)].MelaniamicrostomaLea & Lea, 1851: 186 [type locality: mountain streams, isle of Negros, Philippines].? Melaniaarmillata Lea & Lea, 1851: 195–196 [type locality: India]. ? Melaniabroti Reeve, 1859 in Reeve (1859–1861: pl. 22 fig. 160) [type locality: Ceylon (= Sri Lanka)]. ? Melaniahybrida Reeve, 1859 in Reeve (1859–1861: pl. 13 fig. 163) [type locality: not given]. ? Melaniachocolatum Brot, 1860: 256–257, pl. 16, fig 2 [type locality: “Ceylon” (= Sri Lanka)]. ? Melania (Tiaropsis) rudisvar.spinosa Brot, 1877 in Brot (1874–1879: 306) [type locality: not given, see also Brot (1868: 33, pl. 1, fig. 7)]. ? Melania (Tiaropsis) drilliiformis Martens, 1897: 305 [nomen nudum]. ? Melaniafortitudinis Fulton, 1904: 51–52, pl. 4, fig. 3 [type locality: “Soekaboemi, Java” (= Sukabumi, Java)]. ? Melaniarudisvar.cylindrica Schepman, 1915: 27 [type locality: West Ceram, Kairatu (= West Seram Island, Kairatu)]. Diagnosis.
Thiarid with a turreted, subcylindrical to elongate-ovoid, strongly ornamented high-spired shell with usually rather flattened whorls and a narrowly pyriform aperture that at most reaches half the total shell height, but usually less. Ornamentation of the shell consisting of sinuous axial ribs that usually reach to the base of the body whorl and spiral chords that form nodes where they intersect the ribs; spiral chords usually present on the entire whorl but strongest at the base of body whorl.
Remarks.
The examined type specimens of M.aspera, M.rudis, and M.microstoma correspond well to each other in overall shell shape and sculpture and are here regarded as conspecific because of this. As already noted by Brot (1874–1879: 307) and Glaubrecht and Podlacha (2010: 200), the name Melaniaaspera Lesson, 1830 has priority over the somewhat more frequently used name Melaniarudis (e.g., van Benthem Jutting 1937, 1956; Subba Rao 1989; Ramakrishna and Dey 2007; Budha 2010; Patil and Talmale 2011 as T.rudis). The holotype of Melaniaaspera is unusual in possessing a very small aperture in relation to overall shell height, possibly explaining its isolated position in the PCA 1 vs PCA 2 scatter plot (Fig. 4A). The nominal taxa M.armillata, M.broti, and M.chocolatum described from India or Sri Lanka were regarded by Brot (1874–1879) as closely related to M.rudis and are here tentatively synonymised with M.aspera, largely following the views of Rensch (1934) and van Benthem Jutting (1937, 1956) who synonymised these taxa with M.rudis. According to Brot (1874–1879: 307–308) Melaniahybrida is based on a teratological specimen with an unusual aperture formation and is here tentatively synonymised with M.aspera. The nominal taxon Melania (Tiaropsis) rudisvar.spinosa Brot, 1877 is an individual variation of M.aspera with somewhat longer shoulder spines. The original figures and descriptions of Melaniafortitudinis and M. (Tiaropsis) rudisvar.cylindrica from Java and West Seram Island also correspond well with the holotype of M.aspera and are herein treated as synonyms of the former.
Type material examined.
Holotype of Melaniaaspera Lesson, 1831, MNHN 21098, “La Nouvelle-Guinée”; syntype of Melaniarudis Lea & Lea, 1851, USNM 119778, “Amboyna”; syntype of Melaniamicrostoma Lea & Lea, 1851, USNM 119722, ‘mountain streams, isle of Negros, Philippines’.
Additional material examined
(w: ethanol preserved samples). India: Kolkata, ZMB 107002. Sri Lanka: Colombo, ZMB 107003. Thailand: Samut Sakhon Province, Klong Don Ko, SUT 0311020, ZMB 127535, SUT 0311044, SUT 0311053, ZMB 127534, w; Nakhon Pathom province, Pond in Silpakorn University campus, SUT 0312069, SUT 0312070 = ZMB 127536, w. Indonesia: Bali: South Bali, Yehembang River, ZMB 191279, ZMB 191279a, w, South Bali, at Yehembang, ZMB 106472, w; east of Mendaya, stream southwest of Gumicik, ZMB 191488; Sulawesi: South Sulawesi, Kalena catchment, Angkona river, ZMB 192751, w; southeast Sulawesi, Pohara river, at Pohara, road Kendair to Kolaka, ZMB 191261, w; southeast Sulawesi, Simbune river, 1 km northeast of Raterate, road Kendari to Kolaka, ZMB 191262, ZMB 191262a, w; southeast Sulawesi, stream at Tembeeha, road Tirobus to Kendari, ZMB 191278, w; central Sulawesi, Banggai Islands, Peleng Island, West Peninsula, Tataban river, ZMB 107378, w, ZMB 107377,w. Australia: Northern Territory: Berry Springs, ZMB 106704, w, ZMB 106599a, w, ZMB 127616, w; Wabalaar, Roper River, ZMB 107617, w, ZMB 107614, w, ZMB 127645, w; Salt creek, ZMB 127619, w, ZMB 127636, w, ZMB 127637, w; Roper Bar, ZMB 127620, w; Queensland: O’Shanassy, ZMB 107280.
Shell.
Turreted, subcylindrical to elongate-ovoid, corneous to dark brown, with up to nine whorls (the early whorls usually eroded) (Fig. 1; for juvenile shells, see Fig. 6). Whorls rather flat to convex, separated by a slightly impressed to distinctly impressed, undulating suture. Whorls slightly constricted below the suture, ornamented with sinuous ribs and spiral chords that usually form nodules at their intersections. Radial sculpture usually strongest on the upper half of the whorls, with the nodules at the shoulder of the whorls usually largest, sometimes forming spines. Towards the lower part of the body whorl the spiral sculpture often becomes the dominant sculptural element, forming distinct parallel chords. Aperture pyriform, angled in its upper part and rather narrow, wider at the base and appearing truncated in frontal view. Columella thickened, almost straight to curved, abruptly terminating basally. Shell size H = 7.6–48.0 mm, W = 3.1–22.0 mm (Table 2).
Juvenile and embryonic shells of “Thiara” aspera (Lesson, 1831), SUT 0311020, Samut Sakhon Province, Klong Don Ko. A. Lateral view; B. Apical whorls, lateral, C. Apical view. D. Details of the protoconch. Scale bars: 450 µm (A); 250 µm (B); 200 µm (C); 100 µm (D).
https://binary.pensoft.net/fig/314781
Shell parameters of “Thiara” aspera (Lesson, 1831) specimens from Thailand, Indonesia and Australia, with min./max. values, mean, standard deviation (SD), and number of whorls.
Voucher
Country, region
n
Measurements (mm)
NW
H
W
AL
AW
BW
USNM 119778
Indonesia, Ambon Island
1
23.7
9.7
9.8
5.1
15.5
4
USNM 119722
Philippines, Negros Island
1
20.3
7.7
7.1
3.3
12.3
6
MNHN 21098
Indonesia, West Papua
1
25.0
7.8
6.9
3.5
12.6
7
GSUBg 14265
Indonesia, Java
1
48.0
22.0
22.0
10.0
28.1
7
ZMB 107002
India, Calcutta
1
17.4
7.5
5.7
2.5
12.0
3
ZMB 107003
Sri Lanka, Colombo
5
Range
13.3–16.6
5.3–6.6
4.3–5.3
2.3–2.5
9.0–11.3
4–5
Mean
14.3
5.9
4.8
2.4
9.8
SD
1.2
0.4
0.3
0.1
0.8
SUT 0311053
Thailand, Samut Sakhon
30
Range
7.6–12.8
3.1–5.5
2.8–6.2
1.6–3.4
4.4–7.8
4–7
Mean
9.5
3.9
4.0
2.3
5.7
SD
1.1
0.6
0.6
0.4
0.9
SUT 0311020
Thailand, Samut Sakhon
52
Range
14.1–24.3
7.1–11.1
7.0–11.1
3.2–5.4
9.7–16.2
6–7
Mean
17.7
8.7
8.7
4.2
12.0
SD
2.5
1.0
1.0
0.5
1.5
SUT 0311044
Thailand, Samut Sakhon
1
22.9
10.9
10.5
4.8
15.2
6
SUT 0312070
Thailand, Nakhon Pathom
12
Range
14.4–19.8
5.3–7.9
5.7–6.8
2.4–4.2
7.6–12.0
5–8
Mean
17.1
6.8
7.0
3.4
9.9
SD
1.5
0.7
0.9
0.5
1.2
ZMB 191488
Indonesia, Bali
2
Range
19.8–22.1
8.3–9.4
8.0–8.8
4.1–4.7
12.8–14.5
5
Mean
20.9
8.9
8.4
4.4
13.7
SD
1.1
0.6
0.4
0.3
0.9
ZMB 191278
Indonesia, Sulawesi
19
Range
14.3–18.2
6.2–7.8
6.1–8.9
3.1–3.9
9.3–12.3
4–5
Mean
16.1
6.7
7.2
3.6
10.4
SD
1.0
0.3
0.6
0.2
0.7
ZMB 107377
Indonesia, Sulawesi
10
Range
13.6–20.3
5.5–7.5
4.5–7.3
2.7–4.0
7.7–11.7
4–5
Mean
16.8
6.5
6.2
3.4
9.6
SD
2.1
0.7
0.8
0.4
1.3
ZMB 107378
Indonesia, Sulawesi
19
Range
12.9–19.6
5.2–7.1
5.1–7.8
2.6–3.9
7.7–11.2
4–5
Mean
16.9
6.3
6.3
3.2
9.9
SD
1.5
0.4
0.7
0.4
0.8
ZMB 191279
Indonesia, Bali
17
Range
16.8–27.0
7.0–9.9
7.9–12.0
3.3–5.1
11.0–17.2
4–6
Mean
22.7
8.6
9.9
4.3
14.5
SD
3.7
1.0
1.4
0.6
2.2
ZMB 106472
Indonesia, Bali
16
Range
17.9–22.8
6.4–9.3
7.0–10.6
3.1–5.0
11.4–16.2
4–7
Mean
20.1
7.5
8.5
3.8
12.7
SD
1.4
0.6
0.9
0.4
1.2
ZMB 127538
Indonesia, Bali
20
Range
18.4–30.4
8.6–13.7
9.3–14.9
4.4–7.4
12.8–20.7
4–6
Mean
24.8
11.0
11.8
5.6
16.8
SD
3.0
1.2
1.4
0.7
1.9
ZMB 191268
Indonesia, Sulawesi
5
Range
29.3–41.5
11.5–16.0
12.1–18.7
5.7–8.4
12.3–26.8
4–6
Mean
35.3
13.5
15.3
6.9
20.9
SD
4.9
2.0
2.5
1.1
5.3
Operculum.
The operculum is typical for thiarids, oval and paucispiral, light to dark brown, and with the nucleus being excentric in the lower left corner.
Juvenile shell.
The shells of the juveniles in the brood pouch had up to five whorls, with a maximum height of about 2.5 mm. The protoconch is smooth, with the radial and spiral sculpture developing on the first teleoconch whorls (Fig. 6). For measurements of the embryonic shell, see Table 3.
Measurements of parameters of the juvenile protoconch of “Thiara” aspera (Lesson, 1831) of specimens obtained from the brood pouch.
Voucher
Country, region
n
Measurements (μm)
he
we
de
ZMB 127534
Thailand, Samut Sakhon
3
Range
48.0–63.2
96.0–120.0
312.7–395.7
Mean
54.0
106.7
352.8
ZMB 127535
Thailand, Samut Sakhon
2
Range
56.3–71.4
107.0–114.3
354.3–366.2
Mean
63.9
110.7
352.8
ZMB 191278
Indonesia, Sulawesi
2
Range
34.0–72.4
76.0–91.1
202.0–252.4
Mean
53.2
84.6
227.2
ZMB 191488
Indonesia, Bali
1
–
83.3
95.2
259.5
Radula.
Taenioglossate (Fig. 7), resembling other thiarids. As in all thiarids the central tooth or rachidian is significantly wider than tall; all specimens have a central cusp flanked by three to six triangular denticles on both sides, resulting in up to 12 denticles and a typically 4–5/1/4–5 pattern at the upper cutting edge (Fig. 7A, C, E, Table 4). The laterals are equipped with three to six smaller denticles on the inner side, and three to six denticles outside from the large main cusps (Fig. 7A, C, E, Table 4). The marginal teeth are moderately long, spoon-shaped, with a varying number of 6–10 denticles (Fig. 7B, D, F, Table 4).
Radulae of “Thiara” aspera (Lesson, 1831) from Thailand. A, B.SUT 0312070, Nakhon Pathom province, pond at Silpakorn University campus; A. Central and lateral teeth; B. Marginal teeth; C, D.SUT 0311020, Samut Sakhon province, Klong Don Ko; C. Central and lateral teeth; D. Marginal teeth. E, F: SUT 0311053, Samut Sakhon Province, Klong Don Ko; E. Central and lateral teeth; F. Marginal teeth. Scale bars: 35 µm (A, E); 5 µm (B, F); 25 µm (C); 10 µm (D).
https://binary.pensoft.net/fig/314782
Variation of cusps on the radula teeth of “Thiara” aspera (Lesson, 1831) specimens.
Voucher
Country, region
n
Marginal teeth
Lateral teeth (left)
Lateral teeth (right)
Rachidian
SUT 0311053
Thailand, Samut Sakhon
4
6–8
3-1-3
3-1-3
4–5-1-4–5
SUT 0311020
Thailand, Samut Sakhon
4
6–8
3-1-3
3-1-3
4–5-1-4–5
SUT 0312070
Thailand, Nakhon Pathom
2
7–8
5-1-5
4-1-4
4-1-4
SUT 0312069
Thailand, Nakhon Pathom
2
9–10
3-1-3
3-1-3
3-1-3
ZMB 191278
Indonesia, Sulawesi
2
7–8
3-1-3
3-1-3
4-1-4
ZMB 191488
Indonesia, Bali
1
10
6-1-6
6-1-6
6-1-5
ZMB 191279
Indonesia, Bali
3
6–7
4-1-4
4-1-4
4–5-1-4–5
ZMB 106472
Indonesia, Bali
2
6–7
3-1-3
3-1-3
4-1-4
Reproductive strategy.
The results of the analysis of brood pouch content are summarised in Figure 2. Juveniles of up to 2 mm (rarely also larger) were found in the populations from Thailand, Indonesia (Bali and Sulawesi) and Australia (Northern Territory) suggesting an euviviparous reproductive strategy for “T.” aspera, i.e, the taxon was found to give birth to crawling and shelled juveniles in accordance with the definitions in Glaubrecht et al. (2009). In a few populations in Thailand and on Bali, gravid females with only early embryos, i.e., veliger larvae in the brood pouch were found.
Distribution.
“Thiara” aspera as here understood is a widespread species, with records from Sri Lanka and India (Subba Rao 1989), Myanmar and Cambodia (van Benthem Jutting 1956), Indonesia (Rensch 1934; van Benthem Jutting 1956), and the Philippines (Lea and Lea 1851; van Benthem Jutting 1956). As our results indicate, the taxon is also present in Thailand and northern Australia, from where it was not previously reported (Fig. 2).
Discussion
The results of our phylogenetic analyses show that the nominal taxon Melaniawinteri Busch, 1842 is closely related to Thiaraamarula and can be classified with the same genus. However, the nominal species Melaniaaspera Lesson, 1830 (= Melaniarudis Lea & Lea, 1851), which has often been classified as a member of Thiara (e.g., van Benthem Jutting 1937, 1956; Subba Rao 1989; Ramakrishna and Dey 2007; Budha 2010; Patil and Talmale 2011 under the name T.rudis) cannot be included within that genus on the basis of our data without broadening the concept of Thiara to an extent that it encompasses almost the entire conchological diversity of Thiaridae because “Stenomelania” denisoniensis Brot, 1877, which is conchologically similar to Stenomelania Fischer, 1885 or Melanoides Olivier, 1804, clusters within Thiara s. lat. in our phylogenetic analyses and an approximately unbiased test rejected the monophyly of T.amarula, T.winteri, T.cf.winteri, and “T”. aspera, i.e., excluding “Stenomelania” denisoniensis. Pending a phylogenetic analysis of the entire family, we here retain the species in Thiara, but indicate the tentative placement by quotation marks.
Our phylogenetic analyses further show that “T.” aspera exhibits little genetic variation throughout the Indo-Malayan Archipelago and the Southeast Asian mainland, although populations vary considerably with regard to shell shape, and especially sculpture, confirming previous surveys on thiarid species, which showed also an extraordinary plasticity of the shell (Glaubrecht et al. 2009). We here report the presence of “T.” aspera in Thailand for the first time, albeit in anthropogenic habitats. Previous surveys of the Thai freshwater snail fauna, e.g., by Brandt (1974) did not record the species. Thus, as his years-long surveys were exhaustive it is safe to assume that the species is probably introduced but additional surveys should be carried out to clarify whether the taxon also occurs in natural habitats in this country and was only overlooked in the past.
We also report “T.” aspera here for Australia for the first time, where the taxon was found in natural habitats in the Northern Territory and in north-western Queensland. The populations from Australia were found to be somewhat differentiated genetically from the remaining specimens of “T.” aspera from Thailand and Indonesia included in the phylogenetic analyses and also slightly differ conchologically, i.e., the spiral sculpture almost disappears on the upper half of the teleoconch whorls. Further analysis should therefore confirm whether these differences are constant and would allow a taxonomic separation of the Australian populations.
Unfortunately, no samples could be included in the phylogenetic analyses from either India or Sri Lanka. However, as the examined material closely resembles the holotype of Melaniaaspera in shell characters (although this specimen is exceptional because of its very small aperture in relation to total shell height which may explain its isolate position in Fig. 4A), the populations from these two countries are here regarded as belonging to the species. Therefore, “T.” aspera has to be considered as a widespread species, ranging from India and Sri Lanka across the Southeast Asian mainland and islands into Australia (Fig. 2).
At present, our data on “T.” aspera do not allow to assess whether the observed differences of juvenile stages in the brood pouch of the female indicate differences in the reproductive strategy, or rather individual or seasonal variations. The close phylogenetic relationships among these populations (Fig. 5), however, let the latter two explanations appear more likely in our opinion. Therefore, we consider “T.” aspera a euviviparous species, although it has to be stated that repeated periodic sampling would be necessary to resolve this issue conclusively.
Conclusions
These results highlight the need for a comprehensive revision of the genus-group systematics of Thiaridae as a whole. However, mitochondrial DNA markers are fraud with difficulties in some freshwater cerithioideans (Köhler and Deein 2010; Whelan and Strong 2015; Köhler 2016) and probably also in Thiaridae. Likewise, there appears to be a confusing variability in shell and reproductive features in thiarids, which is in stark contrast to a conserved radular morphology as compared to some other cerithioidean families (Glaubrecht 1996). A stable system of the family, which ought to include the type species of all named genus-group taxa, can be expected to emerge only after phylogenetic analyses based on suitable molecular markers and/or detailed morphological data become available. A stable system of the family then could serve as a basis for a better understanding of the evolutionary systematics and phylogeography of the group.
Acknowledgements
We thank Thomas von Rintelen and Christine Zorn for access to the collection housed at the Museum für Naturkunde, Berlin. We are indebted to the German Academic Exchange Service DAAD for grants in support of research in Thailand and the Deutsche Forschungsgemeinschaft DFG for a grant (DFG GL 297/19-1) making research in Australia possible. We are also indebted to the support fund of the Faculty of Science, Silpakorn University, Thailand and the Thailand Research Fund (The Royal Golden Jubilee Ph.D. Programme PHD/0195/2551) for funding. We thank Vince Kessner (Adelaide River) and Richard Willan (Darwin) for helping with the fieldwork and handling of collections in Australia. We also thank Frank Köhler (Sydney) and Zoltán Fehér (Budapest) for constructive comments on a draft version of the manuscript.
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* a question mark indicates a tentative synonymisation
* a question mark indicates a tentative synonymisation