Bone histology of Hemidactylus long bones. Adult specimen CA2017_001 (cross section no. H63_FR_B5; A, C), ZMB 87078 (cross section no. A12_FL_6b, one year old, SVL 47 mm; B, D, E), and developmental stage I specimen CA2017_015 (F) and CA2017_014 (G). (A–E) Longitudinal sections (HE staining) of the humeral shaft showing a clear pattern of alternating bone lamellae in the periosteal cortex and remodeling in the endosteal region. (C) Close-up of boxed area in (A). (D, E) Magnification of cortical bone of similar areas in (B). (F, G) Onset of ossification in individuals, 25 days before hatching, as seen in histological sections (F) and µCT scans (G). Abbreviations: eb – endosteal bone; f – femur; h – humerus; hl – Howship lacunae; mc – medullary cavity; pb – periosteal bone; r – radius; u – ulna. Scale bars in (A, B) equal 200 µm, in (C–E) 100 µm, in (F) 500 µm, and 1 mm in (G).