Maximum likelihood time-tree inferred from a partitioned analysis of 6626 aligned sites of the mitochondrial genes 12S rRNA, 16S rRNA, and ND1 and the nuclear genes BDNF, C-MYC 2, CXCR4, POMC, RAG1, SLC8A1, and SLC8A3 showing phylogenetic relationships of Centrolene. Support values are shown as percentages next to the branches: SH-aLRT before the slash and ultrafast bootstrap after. The “*” symbol indicates 100%. Node ages in Mya are shown in blue italics (ages lower than 0.5 Mya are not shown). Only the genus Centrolene is presented (a complete tree is available in the Suppl. material 1). Voucher catalogue numbers are displayed before species names. Centrolene kutuku sp. nov. and C. zarza are shown in orange and purple, respectively. The Colombian population of C. huilensis is shown in bold. The complete phylogeny is available as Suppl. material 1.